Western Blot:
- Proficient in performing Western Blot experiments from sample preparation to analysis and interpretation of results.
- Experienced in transferring proteins onto membranes and performing blocking, antibody incubation, and washing steps.
- Knowledgeable in detecting and visualizing protein bands.
- Familiar with troubleshooting potential issues and optimizing Western Blot protocols for improved sensitivity and specificity.
qPCR:
- Competent in performing qPCR assays including primer design, cDNA synthesis, and PCR amplification.
- Experienced in setting up and running qPCR reactions.
Cell Culture:
- Proficient in maintaining and propagating various cell lines.
- Experienced in setting up and running cell-based assays.
- Skilled in performing various cell culture techniques such as cell counting, passaging, freezing, and thawing.
- Familiar with optimizing cell culture conditions.
Flash Freezing Cell Lines:
- Experienced in flash freezing cell lines into pellets using liquid nitrogen for long-term storage and preservation.
- Knowledgeable in handling liquid nitrogen safely and following proper safety protocols.
Other Skills
- Collected, labeled and stored lab samples according to laboratory specifications.
- Used time management and adaptability skills to prioritize and run multiple tests.
- Collected and organized data to perform data analysis and prepare technical reports.
- Performed daily housekeeping tasks driving efficient laboratory operations.
- Supported cleanliness and health standards to foster safe and effective sample analysis.
- Attended lab meetings to participate in research discussions.