Aaron Duenas

Protein purification

• DNA column purification
• RNA column purification
• DNA and RNA purification via oligo DT on Akta Avant

Manufacture Catalog and Custom mRNA

• This involves the production of messenger RNA (mRNA) sequences that are either standard (catalog) or customized to meet specific requirements. Custom mRNA may be designed for research purposes, or therapeutic applications.

DNA Work (PCR and Digest)

• PCR (Polymerase Chain Reaction): A technique used to amplify specific DNA sequences, making millions of copies of a particular DNA segment.
• Digest: Refers to the enzymatic cutting of DNA molecules at specific sequences using restriction enzymes. This process is essential for cloning, analysis, and other molecular biology applications.

DNA Purification

• The process of isolating DNA from cells or other biological materials to remove proteins, lipids, and other contaminants.

Final Fill

• The step where purified DNA is aliquoted into final containers, often under sterile conditions, for storage or distribution.

In Vitro Transcription (IVT)

• A laboratory process used to synthesize RNA molecules from a DNA template. This method is commonly used to produce mRNA for research or therapeutic purposes.

mRNA Purification (RNeasy Columns)

• A technique for purifying mRNA using RNeasy spin columns, which employ a silica membrane technology to selectively bind RNA, allowing for the removal of impurities.

Oligo on ÄKTA Avant

• Oligo is short for oligonucleotides, which are short sequences of nucleotides.
• ÄKTA Avant: A chromatography system used for the purification of proteins, nucleic acids, and other biomolecules.

mRNA Final Fill

• The process of aliquoting purified mRNA into final containers, ensuring sterility and stability for storage or use in downstream applications.

DNA and RNA Gels

• Gel Electrophoresis: A method used to separate DNA or RNA fragments by size through a gel matrix under the influence of an electric field. This technique is essential for analyzing the purity and size of nucleic acids

Technical Review of Batch Records

• Examined for proper documentation of each production batch to ensure manufacturing protocols were followed and to ensure product meets specified quality standards.

Inventory

• Managing the stock of raw materials, reagents, and finished products. This includes tracking quantities, ensuring proper storage conditions, and maintaining accurate records.

Experimental Projects

• Designed and executed experiments to explore scientific questions, develop new techniques, and improve processes. Findings supported company growth by driving cost-effective strategies, faster product delivery, and streamlined operations in DNA and RNA production.

Additional Experience

• Participated in team meetings to discuss production goals, schedules, and quality control issues.
• Maintained lab at a high standard of cleanliness and organization at all times.
• Performed equipment maintenance and calibrations to drive accurate measurements and production of quality products.
• Inspected final products for defects and ensured compliance with industry and SOP standards before packaging for shipment.
• Delivered on-the-job training to new employees regarding production techniques and proper operation of lab equipment.

• Assembled and coated microchannels for oncology diagnostics and also processed, assembled, filtered, and aliquoted materials for aseptic processes
• Maintained appropriate inventory levels of raw materials and consumables used in manufacturing
• Accurately reported Manufacturing Batch Records and adhered to daily production schedules
• Performed specific routine, repetitive manufacturing processes of finished products according to SOP procedures
• Maintained general lab cleanliness and lab equipment
• Maintained effective communication throughout the day with the Production Leader/Designate
• Skilled in maintaining detailed laboratory records

• Company Overview: Worked alongside government entities
• Performed identification and inspection of vegetation along high voltage power lines
• Used aerial photos, GPS data, and satellite maps to locate vegetation plots
• Served as a liaison between clients, tree crews, and Southern California Edison
• Experienced in dealing with sensitive and complex customer issues
• Conducted area audits, inspections, and annual safety stand downs
• Developed and presented incident reports to the broader team, and acted as a safety expert
• Investigated and documented incidents (e.g
• Vehicle and property damage)
• Followed-up with teams to ensure all required training was completed
• Worked alongside government entities

• Participated in after-action reviews, JHA's, and fire discussions such as 6 minutes for safety
• Performed project work such as fuels reduction and road brush removal for forest service roads
• Learned and strengthened traits such as communication, leadership, teamwork, essential PPE, and the importance of safety