Anastasiya Demchuk
Summary
Detail-oriented biology Research Associate with expertise in molecular biology and assay development. Skilled in optimizing protocols, data analysis, and PCR-based techniques to support drug discovery and molecular diagnostics. Proficient in cell culture and immunofluorescence staining while ensuring compliance with GLP and GDP standards. A proactive problem solver and collaborative team player, thriving in fast-paced research environments.
Overview
4
4
years of professional experience
Work History
Biology Research Associate
Kryptos Biotechnologies, Inc.
Hayward, CA
07.2024 - Current
- Performed and optimized multiplex photonic PCR assays, including validation of instruments and consumables, for molecular diagnostics and clinical research applications.
- Performed DNA/RNA extraction and cell culture tasks within BSL-1 and BSL-2 labs, maintaining high safety standards.
- Developed and tested new PCR assays and protocols, collecting and analyzing data using good laboratory practices (GLP), while maintaining detailed records.
- Supported reagent inspection and lyophilization processes to ensure high-quality results and project alignment.
- Collaborated effectively, demonstrating strong communication, adaptability, and problem-solving skills in a fast-paced startup environment.
Associate Scientist II
Merck & Company
South San Francisco, CA
01.2023 - 06.2024
- Independently designed, executed, and troubleshot experiments for target identification and validation, utilizing in vitro cell-based assays to support drug discovery programs.
- Utilized advanced molecular techniques to identify function and action mechanism using tools such as RNA extraction, qRT-PCR, ELISA, Western Blot, cAMP assays, and immunofluorescence staining.
- Interpreted complex datasets using software like Excel and GraphPad Prism.
- Assured data integrity, repeatability, and transparency through precise collection and analysis in digital lab notebook systems like Signals Notebook.
Quality Control Technician I
Lucira Health
San Jose, CA
09.2022 - 10.2022
- Executed comprehensive testing protocols to validate production processes, adhering to standard operating procedures (SOPs).
- Maintained complete, legible, current, and accurate records of all results and procedures performed in a laboratory notebook, following good documentation practices (GDP).
- Prioritized testing for product release, process development testing, and other activities in collaboration with the manufacturing team.
- Evaluated data, drafted reports, noted any relevant deviations from existing standards, and supported root-cause investigations related to nonconformance reports.
PCR Operator, CLIA Laboratory
Verily Life Sciences
South San Francisco, CA
01.2022 - 06.2022
- Executed accurate processing of biological samples in adherence to CLIA laboratory COVID-19 RT-PCR guidelines.
- Transcribed and reviewed sample data in the laboratory information management system, maintaining precise documentation while adhering to PHI/HIPAA standards.
- Managed multiple temperature-specific storage areas to support consistent material availability.
- Utilized advanced robotic machinery to conduct necessary testing and analysis in the lab, such as Agilent Bravo, Tecan Fluent, Thermo KingFisher, QuantStudio 5 PCR System, and others.
Research Associate I, Translational Immunology
Genentech
South San Francisco, CA
06.2021 - 12.2021
- Performed diverse animal handling techniques to facilitate in vivo research, including earmarking, injections (IM/IP/SC/ID), and PO dosing, as well as post-study tissue collection and processing.
- Ensured compliance with IACUC regulations while working with species such as mice and guinea pigs.
- Ensured adherence to health and safety guidelines when handling BSL-2 infectious and biohazardous materials.
- Performed general lab duties to handle the day-to-day operation of the laboratory to ensure smooth operation, such as stocking and ordering supplies, maintaining lab cleanliness, and appropriately disposing of waste.
Research Associate I, QC Bioassays
Revance Therapeutics, Inc.
Newark, CA
09.2020 - 06.2021
- Conducted LD50 animal studies by assisting the principal investigator in the daily preparation of materials for dosing, daily weighing, and scoring of mice and rats after IP and IM dosing completion.
- Assisted with ongoing research projects either independently or as a team member, analyzing and archiving study data.
- Tracked and reported on animal health status to necessary team members.
- Understood and followed relevant Standard Operating Procedures (SOPs) in all laboratory activities, and assisted company personnel with any corrective and preventative action (CAPA) plans.
Education
Master of Science - Genetics
University of The Southwest
Hobbs, NMBachelor of Science - Systems Physiology
San Jose State University
San Jose, CASkills
- Cell culture (adherent, CHO, cultured/primary)
- Polymerase Chain Reactions (qPCR, qRT-PCR, photonic PCR)
- Molecular Techniques (ELISA, Western Blot, cAMP Assay, etc)
- Microtome Tissue Prep, Staining (Fluorescence & Histochemical), and Microscopy
- BSL-1 & BSL-2 Procedures
- Benchling, GraphPad PRISM, Signals Notebook, FIJI/ImageJ, JMP, etc
References
References available upon request.
Timeline
Biology Research Associate
Kryptos Biotechnologies, Inc.
07.2024 - Current
Associate Scientist II
Merck & Company
01.2023 - 06.2024
Quality Control Technician I
Lucira Health
09.2022 - 10.2022
PCR Operator, CLIA Laboratory
Verily Life Sciences
01.2022 - 06.2022
Research Associate I, Translational Immunology
Genentech
06.2021 - 12.2021
Research Associate I, QC Bioassays
Revance Therapeutics, Inc.
09.2020 - 06.2021
Master of Science - Genetics
University of The Southwest
Bachelor of Science - Systems Physiology
San Jose State University
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