Laboratory-trained Scientist offering over 18 years working in the Biotech sector. My working knowledge of bench-work as well as my experience working as part of several interactive, collaborative teams have provided me with the realization that I would like to take the next step in my career. Recognized by leaders and colleagues as an intelligent, driven individual with the ability to master new skills.
Overview
19
19
years of professional experience
Work History
Manufacturing Scientist I
EMD Millipore Corporation
09.2004 - Current
Expert in Protein purification using Biologic, AKTA Pure, AKTA Process, and AKTA Pilot equipment and other scientific methods for Protein A, G, IAP, and absorptions
Fractionate antiserum by Octanoic acid precipitation, Ammonium sulfate fractionation, and phosphate glass
Experience in antibody conjugation by HRP, FITC, CY3, Biotinylation, and production of F(ab’)2 Fragment
Performed complex assays to determine concentration, purity, adhesion, and protein expression
Executed western bolts, cell culture base assay, SDS-PAGE, Heidelberger titers, IEP, Hemoglobin determination, Total Protein determination, electrophoresis/ cellulose acetate, Enzyme immunoassays, as well as other basic immunological and molecular techniques
Perform chemical & buffer formulations, both large and small scale protein purification, sterile and aseptic techniques, pH meter, spectrophotometer, Bio-Rad biologic, and buffer preparation
Experience with the generation, creation, and scheduling of Batch Records using operating systems such as Oracle and QDS
Ensure all work complies with established cCMP, FDA, OSHA, USDA, EPA the ISO Quality system regulations, to assure outputs meet established quality and regulatory specifications
Education
Associate of Science - Mathematics
Mount San Jacinto College
06.2006
Skills
Results Analysis
Team Collaboration
Critical Thinking
Effective Multitasking
Problem-Solving
Cell Culture
Protein expression
Health and Safety Regulations
Routine Laboratory Operations
Safety Processes and Procedures
Research Methods
Application of Scientific Principles
Lab Experiments and Research
Strong Work Ethic
PPE Use
Active Listening
First Aid/CPR
Self-Directed and Motivated
Flow Cytometry
Protein Processing and Harvesting
Molecular Biology
Organizational Skills
Lean manufacturing techniques
Accomplishments
Spot awards in Antibodies, label core, SWAT team, and production
Increased department efficiency and effectiveness by improving order routing, Bill of material updates, and lead time, while on SWAT team