Adrianna Abele
San Diego,CA
Summary
Innovative Researcher adept at developing probing questions, designing experiments, and generating meaningful results. Experienced at identifying and using new technologies and resources. Works effectively and collaboratively with teams and individuals.
Overview
5
5
years of professional experience
Work History
Sr. Research Assoc. I, Synthetic and Molecular Bio
Rejuvenate Bio, Inc.
02.2023 - 06.2023
- Synthesized key information from primary literature, technical reports, and colleagues to bring new ideas to the gene therapy space.
- Developed and executed a series of properly controlled, multifaceted, and economical experiments to support usability of solutions and provide actionable recommendations to the project team under the supervision of a senior scientist.
- Achieved project objectives by troubleshooting experiments and testing practical and creative solutions.
- Communicated project status to the Research and Development Department verbally and through technical documentation and presentations.
- Collaborated with team members to manage multiple concurrent deadlines.
Research Assistant II
Adams Lab, Sanford Burnham Prebys MDI
11.2021 - 02.2023
- Demonstrated ability to work independently and collaboratively.
- Exhibited proficiency in managing multiple projects.
- Presented data at lab meetings.
- Fostered collaborations with other labs.
Teaching Assistant, Molecular Biology
UC San Diego
10.2020 - 08.2021
- Lead discussions with undergraduate students to aid understanding of material presented in lecture by the professor.
- Wrote test material with the guidance of the professor; Evaluated coursework.
- Demonstrated accountability and proficiency in communication.
Research Assistant
David Holway Lab, UC San Diego
05.2018 - 04.2019
- Completed an undergraduate research project.
- Gained experience in field biology, data processing, and the basic form of scientific investigation.
Education
Bachelor of Science - Physiology & Neuroscience
University of California, San Diego
San Diego, CA 2021
Skills
- Cell lines: maintenance of HEK293T, primary human fibroblasts (IMR90), senescent cells
- Aseptic technique; BSL2 safety
- Manual and automated cell counting, tracking Population Doubling Level
- Generation, titration and purification of lentivirus; transduction of IMR90 and senescent IMR90; transfection of HEK293T with AAV; PEI and Lipofectamine transfection
- Restriction cloning; site-directed mutagenesis by inverse PCR; Gibson and Gateway reactions
- Lentiviral, AAV, BAC vector design
- Strategy selection, primer design
- Generation of chemically competent cells
- Analysis of chromatogram data from sanger and whole plasmid PCR sequencing verification and quality control
- Troubleshooting and optimization
Tissue Culture
Molecular Cloning
- RNA isolation, RT-qPCR reactions and primer design
- Bradford Assay, Western Blotting, antibody optimization
- Imaging: Immunofluorescence Assay, Fluorescence Recovery After Photobleaching, Dual Glo Luciferase Assay
- Detailed record keeping in electronic and physical lab notebooks
- Data analysis and interpretation in excel
- Critical analysis of primary literature
- Excellent presentation and writing skills
- Detail-oriented and passionate
- Multi-tasking
- Friendly, team-member
Expression Assays
General
Timeline
Sr. Research Assoc. I, Synthetic and Molecular Bio
Rejuvenate Bio, Inc.
02.2023 - 06.2023
Research Assistant II
Adams Lab, Sanford Burnham Prebys MDI
11.2021 - 02.2023
Teaching Assistant, Molecular Biology
UC San Diego
10.2020 - 08.2021
Research Assistant
David Holway Lab, UC San Diego
05.2018 - 04.2019
Bachelor of Science - Physiology & Neuroscience
University of California, San Diego