Afrah Arshya Fakrurrazi
Rockville,MD
Summary
A passionate Master's student in Pharmaceutical Sciences, deeply interested in tackling complex challenges within the healthcare system through cutting edge research. Driven by a desire to turn ideas into impactful research, with a focus on finding solutions to improve healthcare outcomes. Deep engagement on exploring theoretical aspects behind every practical move, aiming to push the boundaries and drive progress in the field.
Overview
2
2
years of professional experience
Work History
Research Intern
AstraZeneca
06.2024 - Current
- Developing drug-resistant hematological cancer cell line to study resistance mechanisms with current treatment regimens
- Designed and optimized in vitro experiments using cytotoxic assays, flow cytometry to evaluate treatment responses
- Conducted comprehensive literature reviews to inform experimental strategies and troubleshoot technical challenges
- Maintained detailed records of experimental procedures, providing transparency and replicability of results upon request by supervisors or collaborators.
Research Intern
International Center for Biosaline Agriculture
08.2022 - 01.2023
- Salinity level testing for Sorghum Bicolor stress tolerance in lab and field environments
- Conducted salinity stress research on other seed breeds, including quinoa and wheat
- Conducted thorough data analysis on 300 sorghum seed samples to study salinity stress ensuring timely completion of the research objectives
- Performed data entry tasks meticulously, maintaining high levels of accuracy throughout the entire dataset.
Education
MS in Pharmaceutical Sciences -
University of Maryland, Baltimore
Baltimore, MD12.2024
B.E Biotechnology -
BITS Pilani Dubai Campus
Dubai, UAE06.2023
Skills
- Flow Cytometry
- Cytotoxic Assays
- ELISA
- PCR
- Western Blot
- Aseptic cell culture
- SDS-PAGE
- DNA, RNA, Protein & lipid extraction
- Electrophoresis
- Thin Layer Chromatography
- Experimental Design
- FlowJo
- GraphPad Prism
- SnapGene
- PyMol
- AutoDock
- Python
- Microsoft Office Suit
- Protein modeling
- Statistical Analysis
- Data Visualization
- Machine Learning basics
- Technical Reports
- Poster Presentations
- PowerPoint Design
Academic Projects
Effect of Wee-1 Kinase on CDK- 1 phosphorylation, Year 2023
Computational project on Protein-Protein docking to inhibit polo-like kinase, a cancer protein, Year 2022.
- Developed and optimized protocols for investigating the impact of Wee-1 kinase on CDK-1.
- Employed Bradford Assay and Nanodrop spectrophotometry for precise protein concentration measurement.
- Optimized pcdc-2 dilutions through Western blot analysis, enhancing the reliability of experimental results.
- Explored Collagen production in Pichia pastoris by conducting in-depth literature reviews and implementing codon optimization strategies.
- Enhanced collagen production scalability by developing codon optimization solutions using SnapGene, tailored for Pichia pastoris and bacterial systems.
Computational project on Protein-Protein docking to inhibit polo-like kinase, a cancer protein, Year 2022.
- Explored the structure of polo-like kinases and inhibitors using literature reviews, Protein Data Bank and DrugBank database respectively.
- Used computational biological software such as AutoDock, Avogadro, Chimera to identify and understand the docking of inhibitors via its binding energy to inhibit polo-like kinase
Timeline
Research Intern
AstraZeneca
06.2024 - Current
Research Intern
International Center for Biosaline Agriculture
08.2022 - 01.2023
MS in Pharmaceutical Sciences -
University of Maryland, Baltimore
B.E Biotechnology -
BITS Pilani Dubai Campus