Afrina Brishti
Orland Park
Summary
Experienced Pharmacist and ORISE research fellow at GHNRC-ARS-USDA with a focus on investigating the impact of resistance starch and protein content of pulses on substrate oxidation in human subjects. Possess a PhD in Neurobiology and a strong background in discovering brain aging biomarkers and developing glial targeted therapeutics using cell-molecular biology techniques. Postdoctoral research training in neuro-immunology at the University of North Dakota-School of Medicine & Health Sciences, where the relationship between food allergy and brain disorders was explored. Two years of industrial experience in ISO approved pharmaceutical manufacturing facilities. Seeking a career advancement in Neurobiology/Pharmacology/Pharmaceutical Science as a Regulatory Scientist/Reviewer/Investigator/Instructor in industry or academia.
Overview
12
12
years of professional experience
1
1
Certification
Work History
ORISE Post-Doctoral Research Fellow
U.S Department of Agriculture (USDA)
08.2023 - Current
- Company Overview: Human Nutrition Research center
- Developed protocols for ethical human subject recruitment, prepared informed consent form, ensuring compliance with institutional review board requirements, Consent participants and pre-screening.
- Maintained accurate records of experimental procedures, observations, and findings for transparent reporting of results in publications.
- Drafted manuscripts and published in peer-reviewed journals.
- Presented research results at national and international conferences, fostering professional relationships and collaborations.
Post-Doctoral Research Fellow
University of North Dakota
07.2020 - 06.2023
- Company Overview: Department of Biomedical Sciences
- Conceive and design research structure & experimental protocol.
- Enhanced research quality by designing and executing rigorous experiments in a timely manner.
- Implement experimental protocol and perform detailed analysis of scientific data
- Mentored junior researchers and graduate students, fostering their growth as independent scientists.
- Presented research findings at international conferences, increasing visibility within the scientific community.
- Reviewed manuscripts submitted to scientific journals, providing constructive feedback to improve overall quality and rigor.
- Managed laboratory resources efficiently, ensuring optimal use of time and materials for successful project completion.
- Plan and coordinate purchase order management for lab supplies
- Published research articles in subject-specific journals and submitted research grants
Doctoral Fellow
University of Science and Technology of China
08.2015 - 06.2019
- Company Overview: Department of Neurobiology
- Conceive and design experimental protocol
- Collaborated with team to optimize workflow and achieve objectives.
- Implement experimental protocol and perform detailed analysis of scientific data
- Data mining and writing research manuscripts
- Oral and poster presentation of research findings to scientists and extramural communities
- Authored professional scientific papers for publishing in peer-reviewed journals.
Production Officer
Modern pharmaceuticals limited
05.2012 - 03.2015
- Streamlined inventory management, resulting in cost savings and reduced waste.
- Dispensing of pharmaceutical raw materials
- Prepare compliance documentation, safety monitoring protocol
- Improved product quality by closely monitoring production processes and addressing any deviations promptly.
- Prepare Standard Operating Procedure (SOP)
- Trained and supervised production staff, fostering a culture of continuous improvement and high performance.
Education
Ph.D. - Biology-Neurobiology
University of Science And Technology of China
Hefei, Anhui01.2019
MPharm (M.S.) - Pharmacy-Pharmacology
University of Rajshahi
Rajshahi, Bangladesh01.2009
BPharm - Pharmaceutical Sciences
University of Rajshahi
Rajshahi, Bangladesh01.2008
Skills
- Research protocols
- Scientific writing
- Informed consent form preparation
- Laboratory techniques
- Experimental Work Plans
- Computational skills
- Project Management
- Bioinformatics Analysis
- Grant Writing
- Statistical calculation
- Scientific Presentations
Profiles
https://scholar.google.com/citations?user=r6hXTswAAAAJ&hl=en, https://www.linkedin.com/in/afrina-brishti-ph-d-ba1863196/
Current status
USA permanent resident
Training
- Human Research: Group 1.Biomedical Research Investigators and Key Personnel, CITI,USA
- Information Privacy and Security (IPS): Group 1: HIPAA Researchers & Clinicians, CITI,USA
- Information Privacy and Security (IPS): Group 1: HIPAA Researchers & Clinicians, OASH, USA
- Institutional Animal Care and Use Committee (IACUC), UND, USA
- NIH career symposium, NIH, USA
- Principal investigator and key personnel working with rodents, CITI, USA
- Lab Bio-safety training, UND, USA
- Working with mice in research settings, CITI, USA
- AgLearn Trainings assigned for federal employees
- In-plant (Industrial) training (one month) in Beximco Pharmaceuticals Limited, Dhaka, Bangladesh.
Practical laboratory experiences
- Mice sensitization to food allergens (Oral gavage)
- Microdissection of brain and intestine
- Brain embedding in gelatin and sectioning
- Parrafin embedding (intestine tissue array) and sectioning
- Immunohistochemistry
- Frozen tissue sectioning in the cryostat
- Acquire, analyze, and interpret histology data
- Stereotactic brain surgery
- Primary neuron and astrocyte culture
- Co-culture of neuron and astrocyte
- AAV (Adeno Associated Virus) production, purification, and stereotaxic injection into the hippocampus & prefrontal cortex (PFC)
- Western blot assay, Chemiluminescent & Fluorescent
- Brain single cell (microglia/ astrocyte) isolation and sequencing for transcriptomic analysis
- Reporter luciferase assay
- Behavioral assay on Wt and transgenic mice model of different genotypes and aging.
- RNA Immunoprecipitation (IP), RIP
- SA-Beta-Gal staining
- Imuno fluorescence staining including tissue special stains
- Light Microscopic Examination
- Gene Cloning
- ELISA (chemokines, cytokines, and IgGs)
- Fluorescent Multiplexed Bead-Based Immune Assay (FMIA)
- Total and free cholesterol assay
- Lentivirus production and transfection
- Handling different mouse and human cancer cell lines (U87, SHSY5Y, N2A, IMR90, 293T, HELA, HEPG) for transfection
- Fluorescence in situ hybridization (FISH)
- Release pattern analysis of enteric-coated drug, in vitro (Pharmacokinetics)
- Drug efficacy measurement, in vivo (Pharmacodynamics)
- Software: Qupath, CANVAS 14, Graph pad Prism, ImageJ, Fiji, Adobe Photoshop, Biorender, ANYmaze.
Certification
- CPR/AED Certification
- OSHA Certified
Languages
Bengali
Native or Bilingual
Chinese (Mandarin)
Limited Working
English
Full Professional
Arabic
Elementary
Hindi
Elementary
Interests
- Cooking
- Knitting and Crocheting
- Gardening
- Adventure Travel
Timeline
ORISE Post-Doctoral Research Fellow
U.S Department of Agriculture (USDA)
08.2023 - Current
Post-Doctoral Research Fellow
University of North Dakota
07.2020 - 06.2023
Doctoral Fellow
University of Science and Technology of China
08.2015 - 06.2019
Production Officer
Modern pharmaceuticals limited
05.2012 - 03.2015
- CPR/AED Certification
- OSHA Certified
Ph.D. - Biology-Neurobiology
University of Science And Technology of China
MPharm (M.S.) - Pharmacy-Pharmacology
University of Rajshahi
BPharm - Pharmaceutical Sciences
University of Rajshahi
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