Agbor Otu Egbe Vydaline
Yonkers,NY
Summary
Goal-oriented biochemists with outstanding communication, organizational, and multitasking abilities. A highly motivated employee with a desire to take on new challenges. Strong work ethic, adaptability, and exceptional interpersonal skills. Adept at working effectively unsupervised and quickly mastering new skills. Meticulous expertise in designing and performing techniques for ligand-guided selection of nucleic acid aptamers against cell surface receptors, polymerase chain reaction, Next-generation sequencing, and bioinformatics analysis of genome and protein sequences.
Overview
10
10
years of professional experience
Work History
PhD Biochemistry
Gradcenter CUNY
New York, NY
08.2020 - Current
- I led the team to successfully deliver project requirements, develop solutions, and meet deadlines.
- Prioritized and organized tasks to efficiently accomplish service goals.
- I have experience in designing, executing, and documenting scientific experiments for research purposes.
- Optimize equipment through rigorous calibration and maintenance to guarantee accurate and precise results.
- Selecting DNA aptamers that bind to cell surface receptors in their natural state.
- DNA synthesis, precipitation, and purification using reverse-phase HPLC
- Detritylation and G-column desalting of purified DNA aptamers
- UV-Vis spectrophotometry quantification of DNA aptamers
- Agarose gel electrophoresis and visualization
- Maintained human cell line cultures (Jurkat cells, HEK293 cells, HeLa cells)
- Carried out binding, affinity, and specificity assay of aptamers to cells
- Antibody staining, T-cell receptor activation assay, and flow cytometry analysis using Jurkat E6cells
- PCR, qPCR and NGS
- Confocal microscopy, fluorescent microscopy and light microscopy visualization and analytical techniques
Adjunct Lecturer
CUNY school of Medicine, City College
NEW YORK, New York
01.2024 - Current
- Facilitated group discussions among students to promote critical thinking skills.
- Maintained accurate records of attendance and grades for each student in classes taught.
- Promoted an environment of respect, openness, collaboration, and engagement within the classroom setting.
Adjunct Lecturer
Lehman College, CUNY
The Bronx, NY
08.2021 - 09.2023
- Utilized diverse teaching methods, lectures, presentations and class activities to deliver curriculum.
- Organized activities and class events to drive learning objectives and promote engagement.
- Created lesson plans and developed instructional materials covering required topics and learning objectives.
- Lectured to classes of up to 20 students regarding laboratory organic chemistry.
- Documented student assignments, attendance and test scores in online reporting systems.
Research Assistant
Rega institute for medical research
Leuven, BELGIUM
07.2018 - 09.2019
- Conducted experiments and maintained detailed records of experiments and outcomes.
- Designed CRISPR gRNA and templates using the ApE software
- Expressed the gRNA in E.coli via transformation.
- Transfected HEK293T cells with the CRISPR construct and viral infection.
- Analyzed cell mutations in different viral clones relation to herpes virus infection
- PCR amplification of the DNA polymerase of viral clones, agarose gel electrophoresis
- Bigdye reaction and Sanger sequencing of viral clones
- Analysis of sequencing data
Medical Laboratory Technician
The District Hospital
MAMFE, Cameroon
08.2014 - 06.2016
- Followed laboratory guidelines for handling biological specimens.
- Followed strict aseptic techniques to protect patients when collecting blood and tissue samples.
- Supported the work of pathologists by cutting, staining, and mounting tissue samples for analysis.
- Drew blood from patients to collect samples using venipuncture and following aseptic techniques.
- Adhered to all safety and infection control precautions and regulations.
- Documented patient medical information while following privacy procedures.
- Carried out medical laboratory procedures using microscopy, microbiology, Biochemistry, and serology skills.
- Mass outreach sensitization on cervical cancer and screening of samples using histopathological staining techniques (Papanicolaou Stain).
Education
Master of Science - Molecular Biology
Vrije Universiteit, Brussel
Belgium09.2019
Bachelor of Science - Biochemistry And Medical Laboratory Technology
University of Bamenda
Cameroon08.2016
Ph.D. - Biochemistry
Graduate Center CUNY
New YorkSkills
- Designing and executing experiments
- Wetlab techniques like Flow cytometry, nucleic acid ligand selection, next-generation sequencing, Real-time polymerase chain reaction, cell culture, gel electrophoresis, HPLC, UV-Vis Spectroscopy, etc
- DNA synthesis, precipitation, purification, and quantification
- Drug internalization assays and Protein pull-down assays
- Cell culture, cell media preparation, and laboratory aseptic techniques
- FASTAptamer and GALAXY Bioinformatics analysis of Nucleic acid and protein sequences
- Confocal microscopy
- Public speaking strategies
- Installation and equipment maintenance
- Lab Management/Record keeping
- Quality Assurance
Languages
English
Native/ Bilingual
French
Limited
Affiliations
- Ph.D Biochemistry Executive committee student representative (2021-2023)
- Pioneer class coordinator of the Biochemistry department (2013-2016)
- Member of the faculty of Science students association (FASSA 2013-2016)
- Students Class council representative Vrije Universiteit Brussel (2017-2018
- IPMB Alumni association
Adward
IS-CUNY Science Scholar Fellowship (2023-2025)
IS-Chemistry Tithe Fellowship (2022-2025)
CUNY Science scholarship (2020-current)
Presidential award for best Biochemistry graduate(2016)
Sumbele academic excellence award (2012-2014)
Hobbies
Good music
Lawn tennis
Reading Novels
Timeline
Adjunct Lecturer
CUNY school of Medicine, City College
01.2024 - Current
Adjunct Lecturer
Lehman College, CUNY
08.2021 - 09.2023
PhD Biochemistry
Gradcenter CUNY
08.2020 - Current
Research Assistant
Rega institute for medical research
07.2018 - 09.2019
Medical Laboratory Technician
The District Hospital
08.2014 - 06.2016
Master of Science - Molecular Biology
Vrije Universiteit, Brussel
Bachelor of Science - Biochemistry And Medical Laboratory Technology
University of Bamenda
Ph.D. - Biochemistry
Graduate Center CUNY
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