Summary
Overview
Work History
Education
Skills
Publications
Timeline
Generic

Andrew Makowski

Kelley,IA

Summary

Dedicated Life Scientist with experience in laboratory research and management. Expertise in data analysis and background in analytical chemistry, material science and food chemistry. Proficient in advanced laboratory equipment use and techniques. Detail-oriented team player with strong organizational skills. Ability to handle multiple projects simultaneously with a high degree of accuracy.

Overview

16
16
years of professional experience

Work History

Research Scientist – Lab Manager

Iowa State University
01.2023 - Current
  • Method development, Method Validation, and SOP writing
  • QC/QA manager
  • Manage both technical and non-technical staff
  • Client liaison, key account manager, procurement manager, and asset manager
  • TTB certification for the import and export of wine and beer
  • Serviced and maintained HPLC, GC-FID, SPME-GC-MS, GC-MS-O systems for various aroma and chemical profiles
  • Winemaker

Owner/Operator

Cyclone Country Stables LLC
08.2020 - Current
  • Client Liaison
  • Accountant, Business manager
  • Equine/Barn manager
  • Farrier


Principal Investigator

Nistron LLC
09.2021 - 12.2022
  • Technology research and development
  • Leadership and Management
  • Product commercialization
  • NSF and NIH program manager and grant author
  • Hydrogel-based organ-on-a-chip technologies for in-vitro biological barriers for pharmaceutics and disease modeling.

Analytical Chemist- LCMS Specialist

Heartland Assays LLC & Metabolic Technologies Inc.
05.2012 - 09.2021
  • Assistant Technical supervisor
  • Research and development of Vitamin D assays for LCMSMS and HPLC
  • Method Validation and development for client services, client liaison, and account rep
  • LCMSMS, HPLC, Spectrophotometer, Radioactive immunoassays, solid phase extraction, general wet chemistry, synthesis, radioactive isotopes
  • Bloodborne pathogens, CLIA compliant, ISO 17025, GLP
  • Laboratory information Management system Administrator

Lab Research Chemist/Physicist/Process Engineer

3M- Renewable Energies Division (Aerotek Scientific)
08.2010 - 04.2012
  • Formulation of new adhesive technologies for solar module applications
  • Production mach-up with emphasis on process engineering and extrusion techniques and formulation, TSE, SSE
  • Optical, adhesive, tensile, electrical, and aging testing
  • ISO 9001
  • Lean Six Sigma
  • MTS, DMA, Aries, Lambda 900, Macbeth color-eye 2180, % Crosslinking
  • Lotus notes, MS Office, laboratory notebooks.

Research Associate – Conjugated Polymer

Iowa State University
05.2008 - 12.2009
  • Multi-step synthesis, purification, and development of conjugated monomer units for incorporation into various poly(benzobisoxazole) conjugated polymers
  • Optimized syntheses of conjugated monomer units for collaboration with other projects
  • Characterized products by 1H and 13C NMR, GC/MS, HPLC, UV-vis and Fluorescence spectroscopy
  • Managed chemical inventory and drafted SOP’s
  • Trained incoming students and researchers.

Research Associate – Silica and Titanium Nanoparticles

Iowa State University
05.2008 - 12.2009
  • Designed and tested new experimental procedures for titanium-based mesoporous nanoparticles
  • Repeat synthesis of mesoporous silica nanoparticles (MSNs) and quantification via XRD.

Education

Bachelor of Arts, Chemistry -

Iowa State University of Science And Technology
Ames, IA
12.2009

Skills

  • Research Planning
  • Root Cause Analysis
  • Experiment Design
  • Clinical Assays
  • Research Methods
  • Quality Assurance Controls
  • Research and Publication
  • Data Analysis
  • Research Grants
  • Method Validation
  • Results Interpretation
  • Technical Troubleshooting

Publications

1- Mike, J.F.; Makowski, A.; Jeffries-EL, M. An Efficient Synthesis of 2,6-Disubstituted Benzobisoxazoles: New Building Blocks for Organic Semiconductors. Org. Lett, 2008, 10(21), 4915. DOI: 10.1021/ol802011y 


2- Mike, J.F.; Makowski, A.; Jeffries-EL, M. Synthesis and Characterization of Dialkoxy Substituted Poly(phenylenevinylene) Benzobisoxazoles. J. Poly. Sci. A, 2010, 48 (6), 1456-1460.DOI: 10.1002/pola.23869. 


3- Mike, J F.; Nalwa, K.; Makowski, A. J.; Putnam, D.; Tomlinson A.; Chaudhary, S. and Jeffries-EL, M. Synthesis, Characterization and Photovoltaic Properties of Poly(thiophenevinylene) Benzobisoxazoles. Phys. Chem. Chem. Phys. 2011, 13, 1338-1344. DOI:10.1039/C0CP00353. 


4- Diet-derived 25-hydroxyvitamin D3 activates vitamin D receptor target gene expression and suppresses EGFR mutant non-small cell lung cancer growth in vitro and in vivo. Verone-Boyle A.R. Shoemaker S. Attwood K. Morrison C.D. Makowski A.J. Battaglia S. Hershberger P.A. Oncotarget 7, 995-991013 (2016) 


5- Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry. 


6- Roseland J.M., Patterson K.Y., Andrews K.W., Phillips K.M., Phillips M.M., Pehrsson P.R., Dufresne G.L., Jakobsen J, Gusev P.A., Savarala S, Nguyen Q.V., Makowski A.J., Scheuerell C.R., Larouche G.P., Wise S.A., Harnly J.M., Williams J.R., Betz J.M., Taylor C.L. J Agric Food Chem. 2016 Apr 27;64(16):3167-75. doi: 10.1021/acs.jafc.5b05016. Epub 2016 Apr 15. 


7- Prostatic compensation of the vitamin D axis in African American men. Zachary Richards, Ken Batai, Rachael Farhat, Ebony Shah, Andrew Makowski, Peter H. Gann, Rick Kittles, and Larisa Nonn. JCI Insight. 2017 Jan 26; 2(2): e91054. Published online 2017 Jan 26. doi: 10.1172/jci.insight.91054 


8- Simplified 25-Hydroxyvitamin D Standardization and Optimization in Dried Blood Spots by LC-MS/MS. Makowski A.J., Rathmacher J.A., Horst R.L., Sempos C.T. J AOAC Int. 2017 May 11. doi: 10.5740/jaoacint.17-0086. 


9- Influence of Various Factors on Circulating 25(OH) Vitamin D Concentrations in Dogs with Cancer and Healthy Dogs. Weidner, N., Woods, J.P., Conlon, P., Meckling, K.A., Atkinson, J.L., Bayle, J., Makowski, A.J., Horst, R.L. and Verbrugghe, A. (2017),. J Vet Intern Med, 31: 1796–1803. doi:10.1111/jvim.14834 


10- Sun exposure in pigs increases the vitamin D nutritional quality of pork. Larson-Meyer D.E., Ingold B.C., Fensterseifer S.R., Austin K.J., Wechsler P.J., Hollis B.W., Makowski A.J., Alexander B.M. PLoS One. 2017 Nov 14;12(11):e0187877. doi: 10.1371/journal.pone.0187877—eCollection 2017. 


11- SEASONAL VARIATION OF SERUM 25-HYDROXY-VITAMIN D IN TWO CAPTIVE EASTERN BLACK RHINOCEROS (DICEROS BICORNIS MICHAELI) HOUSED IN A NORTH AMERICAN ZOO. June Olds, Wes Oltman, Andrew J. Makowski, Hilary Householder, and Lou L. Keeley (2018). Journal of Zoo and Wildlife Medicine: December 2018, Vol. 49, No. 4, pp. 943-951. 


12- The Effects of Maternal Dietary Supplementation of Cholecalciferol (Vitamin D3) and 25(OH)D3 on Sow and Progeny Performance. T Thayer, Morgan & L. Nelssen, J & J. Langemeier, A & Morton, J & M. Gonzales, J & R. Kruger, S & Ou, Zhining & Makowski, Andrew & Bergstrom, Jonathan. (2019). Kansas Agricultural Experiment Station Research Reports. 4. 10.4148/2378-5977.7650. 


13- Obesity Decreases Hepatic 25‐Hydroxylase Activity Causing Low Serum 25‐Hydroxyvitamin D. Roizen, J. D., Long, C. , Casella, A. , O'Lear, L. , Caplan, I. , Lai, M. , Sasson, I. , Singh, R. , Makowski, A. J., Simmons, R. and Levine, M. A. (2019), J Bone Miner Res. doi:10.1002/jbmr.3686 


14- EVALUATION OF 25-HYDROXYVITAMIN D IN HOFFMANN'S TWO-TOED SLOTHS (CHOLOEPUS HOFFMANNI) USING DRIED BLOOD SPOTS ANALYZED BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY. Higgins JL, Scanlon LM, Makowski AJ, Childs-Sanford SE. J Zoo Wildl Med. 2020 Jan 9;50(4):751-757. doi: 10.1638/2019-0045. PMID: 31926504. 


15- THE VITAMIN D STATUS OF ASIAN ELEPHANTS (ELEPHAS MAXIMUS) MANAGED IN A NORTHERN TEMPERATE CLIMATE. Childs-Sanford SE, Makowski AJ, Wakshlag JJ. J Zoo Wildl Med. 2020 Mar 17;51(1):1-12. doi: 10.1638/2019-0097. PMID: 32212541. 


16- Vitamin D status in cats with cardiomyopathy. Ware, WA, Freeman, LM, Rush, JE, Ward, JL, Makowski, AJ, Zhang, M. J Vet Intern Med. 2020; 34: 1389– 1398. https://doi.org/10.1111/jvim.15833 


17- Vitamin D Content in Milk of the Rhesus Monkey. Alan M. Preston, Andrew J. Makowski, Melween I. Martinez. PRHSJ 2021 Mar. Vol. 40 No. 1 


18- Kramer, A., Thomas, M., Makowski, AJ., & Drozek, D. (2021). The Prevalence of Vitamin D Deficiency in Impoverished Communities in Northern Lima, Peru. International Journal of Disease Reversal and Prevention, 3(2), 10 pp. https://doi.org/10.22230/ijdrp.2021v3n2a257 


19- Jimenez, I. A., Makowski, A. J., Scanlon, L. M., & Childs-Sanford, S. E. (2021). Cutaneous concentrations of 7-dehydrocholesterol in Hoffmann's two-toed sloths (Choloepus hoffmanni) in managed care. Zoo Biology, 1– 6. https://doi.org/10.1002/zoo.21643 


20- Nima Alimoradi, Vahid Nasirian, Saurabh S. Aykar, Marilyn C. McNamara, Amir Ehsan Niaraki-Asli, Reza Montazami, Andrew J. Makowski, Nicole N. Hashemi. (2022), Fabrication of Conductive Hollow Microfibers for Encapsulation of Astrocyte Cells. bioRxiv 2022.03.09.483669; doi: https://doi.org/10.1101/2022.03.09.483669 


21- Sara E. Childs-Sanford, Andrew J. Makowski, Rachel L. Hilliard, Joseph J. Wakshlag "EXPERIMENTAL CHOLECALCIFEROL SUPPLEMENTATION IN A HERD OF MANAGED ASIAN ELEPHANTS (ELEPHAS MAXIMUS)," Journal of Zoo and Wildlife Medicine, 54(2), 219-230, (7 July 2023) 


22- Ouedraogo, L. J., Trznadel, M. J., Kling, M., Nasirian, V., Borst, A. G., Shirsavar, M. A., Makowski, A., McNamara, M. C., Montazami, R., Hashemi, N. N., Hydrodynamic Assembly of Astrocyte Cells in Conductive Hollow Microfibers. Adv. Biology 2023, 2300455. https://doi.org/10.1002/adbi.202300455

Timeline

Research Scientist – Lab Manager

Iowa State University
01.2023 - Current

Principal Investigator

Nistron LLC
09.2021 - 12.2022

Owner/Operator

Cyclone Country Stables LLC
08.2020 - Current

Analytical Chemist- LCMS Specialist

Heartland Assays LLC & Metabolic Technologies Inc.
05.2012 - 09.2021

Lab Research Chemist/Physicist/Process Engineer

3M- Renewable Energies Division (Aerotek Scientific)
08.2010 - 04.2012

Research Associate – Conjugated Polymer

Iowa State University
05.2008 - 12.2009

Research Associate – Silica and Titanium Nanoparticles

Iowa State University
05.2008 - 12.2009

Bachelor of Arts, Chemistry -

Iowa State University of Science And Technology
Andrew Makowski