Alayna Hawkins-Gigliotti

King Of Prussia,PA

Summary

Enthusiastic researcher with 2 years experience working in gene therapy field and 4 years of molecular biology research experience. Broadly, my skills consist of AAV-vector production from vector design to titering assay development, mammalian cell-culture techniques and various methods of protein quantification.

I display strong written and oral communication skills and am an adaptive team player with in-depth knowledge of designing, executing and analyzing scientific data.

Overview

years of professional experience

years of post-secondary education

Work History

Senior Research Associate

NeuExcell Therapeutics Inc.

Philadelphia, PA

11.2020 - Current

Initiated, planned and executed research projects for a gene therapy company specializing in AAV-based vectors to treat neurodegenerative diseases
Achieved project objectives by troubleshooting experiments and testing practical and creative solutions.
Collaborated with leadership team to identify relevant questions and determine best methods of collection.

Education

Master of Science - Biology

The University of Maine

08.2018 - 08.2020

Bachelor of Science - Biology

Saint Francis University

08.2014 - 05.2018

Skills

Titering via: RT-PCR, qPCR, dPCR

Mammalian cell culture: maintenance, transfection and transduction

Protein quantification: SDS-PAGE Western Blot, In-Cell Western, ELISA

AAV Production and purification

DNA extraction, purification and cloning

RNA extraction and purification

Bioinformatics: proficient in R statistical programming software

Data analysis via Prism

Publication

Gigliotti, A. K., Bowen, W. D., Hammill, M. O., Puryear, W. B., Runstadler, J., Wenzel, F. W., & Cammen, K. M. (2022). Sequence Diversity and Differences at the Highly Duplicated MHC-I Gene Reflect Viral Susceptibility in Sympatric Pinniped Species. Journal of Heredity, esac030. https://doi.org/10.1093/jhered/esac030

Timeline