Aleksandra Jovanovic
Oakland,CA
Summary
Dedicated professional with over ten years of expertise in the field of metabolic disorders and cardiovascular diseases. Extensive experience in a wide range of experimental techniques, including animal handling, metabolic phenotyping and molecular biology assays. Comfortable handling complex issues, meeting strict deadlines and adjusting to rapidly changing conditions.
Overview
12
12
years of professional experience
Work History
Postdoctoral Researcher
University of California
Berkeley
05.2022 - Current
- Independently designed and executed experiments to investigate the function of primary cilia in choroid plexus epithelial cells.
- Utilized confocal microscopy to acquire high-resolution imaging data to visualize and analyze protein localization and expression patterns in tissue explant.
- Adapted and optimized FRET assay to study cAMP production in choroid plexus tissue explant.
- Managed and maintained mouse colonies, performed genotyping to identify transgenic, knockout, or genetically modified mouse lines using PCR-based methods.
- Mentored undergraduate and graduate students involved in research projects.
- Collaborated closely with internal teams and external collaborators to leverage expertise and resources for advancing research objectives.
Postdoctoral Researcher
University of California
Davis
08.2019 - 05.2022
- Conducted in vivo and in vitro assays to characterize metabolic phenotype in mice or cells.
- Led the development of a transgenic mouse model with fat tissue-specific deletion of beta-2 adrenergic receptor (b2AR), enabling investigation into the role of b2AR in adipocyte biology and metabolic regulation.
- Conducted FRET based methods in both cell lines and primary cells (including cardiomyocytes and neurons).
- Performed molecular biology methods including gene manipulation and cloning.
- Authored professional scientific papers for publishing in peer-reviewed journals.
- Presented data at lab meetings and journal clubs.
Research Assistant
Laboratory for Radiobiology and Molecular Genetics, Institute of Nuclear Sciences “Vinca
Belgrade, Serbia
11.2012 - 08.2019
- Conducted experiments both independently and within the team.
- Performed biochemical assays to characterize phenotypes using Western Blot, PCR and ELISA methods.
- Kept detailed records of laboratory research.
- Analyzed data statistically.
- Wrote research articles for various national and international journals.
- Presented plans and data with scientists and laboratory staff on regular basis.
- Attended numerous conferences, seminars and round tables related to the area of my research.
Education
PhD Degree - Biology, Animal and Human Physiology
Faculty of Biology, University of Belgrade
Belgrade, Serbia02.2019
Master’s Degree - Biology, Animal Physiology
Faculty of Biology, University of Belgrade
Belgrade, Serbia09.2012
Skills
- Confocal microscopy
- Förster resonance energy transfer (FRET) based methods
- Animal handling and in vivo experiments
- Western blot
- ELISA
- Cell culture
- PCR
- Gene editing techniques using both plasmid transfection and viral infection methods
Additional Information
Publications:
1. Aleksandra Jovanovic, Bing Xu, Chaoqun Zhu, Di Ren, Hao Wang, Meredith Krause-Hauch, E. Dale Abel, Ji Li, Yang K. Xiang. CharacterizingAdrenergic Regulation of Glucose Transporter 4-Mediated Glucose Uptake and Metabolism in the Heart. J Am Coll Cardiol Basic Trans Science. Feb 22, 2023. Epublished DOI: 10.1016/j.jacbts.2022.11.008
2. Joseph M. Martinez, Ao Shen, Bing Xu, Aleksandra Jovanovic, Josephine de Chabot, Jin Zhang, Yang K. Xiang. Arrestin dependent nuclear export of phosphodiesterase 4D promotes GPCR-induced nuclear cAMP signal in learning and memory. Science Signaling. (2023). In press.
3. Ying Wang, Meimi Zhao, Bing Xu, Sherif MF Bahriz, Chaoqun Zhu, Aleksandra Jovanovic, Haibo Ni, Ariel Jacobi, Nina Kaludercic, Fabio Di Lisa, Johannes W Hell, Jean C Shih, Nazareno Paolocci, Yang K Xiang. Monoamine oxidase A and organic cation transporter 3 coordinate intracellular β1AR signaling to calibrate cardiac contractile function. Basic Research in Cardiology, (2022); volume 117, Article number: 37
4. Rui Xu, Raghavender Reddy Gopireddy, Yudi Wu, Lei Wu, Xiang Tao, Ji Shao, Wenxin Wang, Li Li, Aleksandra Jovanovic, Bing Xu, Nicolas J Kenyon, Quan Lu, Yang K Xiang, Qin Fu. Hyperinsulinemia Promotes Heterologous Desensitization of β2 Adrenergic Receptor in Airway Smooth Muscle in Obesity. FASEB J. 2020 Mar;34(3):3996-4008. doi: 10.1096/fj.201800688RR. Epub 2020 Jan 21.
5. Ivana Resanovic, Zoran Gluvic, Bozidarka Zaric, Emina Sudar-Milovanovic, Aleksandra Jovanovic, Davorka Milacic, Radmilo Isakovic, and Esma R. Isenovic. Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study. International Journal of Endocrinology, vol. 2019, Article ID 2328505, 12 pages, 2019. https://doi.org/10.1155/2019/2328505.
6. Sonja Zafirovic, Milan Obradovic, Emina Sudar Milovanovic, Aleksandra Jovanovic, Julijana Stanimirovic, Alan J. Stewart, Samantha J. Pitt, Esma R. Isenovic. 17ß- Estradiol protects against the effects of a high fat diet on cardiac
glucose, lipid and nitric oxide metabolism in rats. Molecular and Cellular Endocrinology (2017); 446:12- 20.
7. Sudar-Milovanovic E, Zafirovic S, Jovanovic A, Trebaljevac J, Obradovic M, Cenic- Milosevic D, Isenovic ER. Hormonal Regulation of Nitric Oxide (NO) in Cardio- metabolic Diseases. Current Pharmaceutical Design, 2017; 23(10):1427-1434.
8. Aleksandra Jovanovic, Milan Obradovic, Emina Sudar Milovanovic, Alan J. Stewart, Samantha J. Pitt, Dragan Alavantic, Ema Aleksic and Esma R. Isenovic (2017): Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet. Molecular and Cellular Biochemistry. 436(1-2): 49-58.
9. Aleksandra Jovanovic, Emina Sudar-Milovanovic, Milan Obradovic, Samantha J Pitt, Alan J Stewart, Sonja Zafirovic, Julijana Stanimirovic, Djordje Radak and Esma R Isenovic. Influence of a High-Fat Diet on Cardiac iNOS in Female Rats. Current Vascular Pharmacology (2017); 15(5):491-500.
10. Radak D, Katsiki N, Resanovic I, Jovanovic A, Sudar-Milovanovic E, Zafirovic S, Mousad SA, Isenovic ER1. Apoptosis and Acute Brain Ischemia in Ischemic Stroke. Current Vascular Pharmacology (2017); 15(2):115-122.
11. Julijana Stanimirovic, Milan Obradovic, Aleksandra Jovanovic, Emina Sudar- Milovanovic, Sonja Zafirovic, Smantha J. Pitt, Alan J. Stewart and Esma R. Isenovic. A high fat diet induces sex-specific differences in hepatic lipid metabolism and nitrite/nitrate in rats. Nitric Oxide (2016); 54:51-9.
12. Emina Sudar Milovanovic, Milan Obradovic, Aleksandra Jovanovic, Bozidarka Zaric, Sonja Zafirovic, Anastasija Panic, Djordje Radak, Esma R. Isenovic. Benefits of L- Arginine on Cardiovascular System. Mini Rev Med Chem. 2015; 16(2):94-103.
13. Emina Sudar, Aleksandra Jovanovic, Maja Misirkic-Marjanovic, Ljubica Vucicevic, Kristina Janjetovic, Esma R. Isenovic. Effects of intracerebroventricularly (ICV) injected ghrelin on cardiac inducible nitric oxide synthase activity/expression in obese rats. Exp Clin Endocrinol Diabetes. 2015 Nov123 (10):581-8.
14. Milan Obradovic, Sonja Zafirovic, Aleksandra Jovanovic, Emina Sudar Milovanovic, Shaker A. Mousa, Milica Labudovic-Borovic and Esma R. Isenovic. Effects of 17β- estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats. Mol Cell Endocrinol. 2015 Nov 15; 416:46-56.
15. Zoran Gluvic, Emina Sudar, Jelena Tica, Aleksandra Jovanovic, Sonja Zafirovic, Ratko Tomasevic and Esma R. Isenovic.Effects of levothyroxine replacement therapy on parameters of metabolic syndrome and atherosclerosis in
hypothyroid patients: a prospective pilot study. Int J Endocrinol. 2015; 2015:147070. doi: 10.1155/2015/147070.
16. Obradovic MM, Trpkovic A, Bajic V, Soskic S, Jovanovic A, Stanimirovic J, Panic M, Isenovic ER. Interrelatedness between C-reactive protein and oxidized low-density lipoprotein. Clin Chem Lab Med. 2015 Jan 1; 53(1):29-34.
Timeline
Postdoctoral Researcher
University of California
05.2022 - Current
Postdoctoral Researcher
University of California
08.2019 - 05.2022
Research Assistant
Laboratory for Radiobiology and Molecular Genetics, Institute of Nuclear Sciences “Vinca
11.2012 - 08.2019
PhD Degree - Biology, Animal and Human Physiology
Faculty of Biology, University of Belgrade
Master’s Degree - Biology, Animal Physiology
Faculty of Biology, University of Belgrade
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