Alexis Nicole Brumwell

Experienced scientist with extensive hands-on expertise in flow cytometry in a collaborative academic laboratory.

• Train postdoctoral research, graduate students, and research associates in basic and specialized lab techniques as well as how to conceptualize successful experiments.
• I supervise all flow cytometry performed in lab. I aid and teach lab members and collaborators acquisition, machine setup, troubleshooting, experiment design, and creation of color panels.
• Train researchers in multiple labs across UCSF how to efficiently isolate lung epithelial cells from both mouse and human lungs

1. 2024 Max L Cohen, Alexis N Brumwell, Tsung Che Ho, Kiana Garakani, Genevieve Montas, Darren Leong, Vivianne W Ding, Jeffrey A Golden, Binh N Trinh, David M Jablons, Michael A Matthay, Kirk D Jones, Paul J Wolters, Ying Wei, Harold A Chapman, Claude Jourdan Le Saux.A fibroblast-dependent TGF-β1/sFRP2 noncanonical Wnt signaling axis promotes epithelial metaplasia in idiopathic pulmonary fibrosis. Journal of Clinical Investigation, 2024.

Authorship note: MLC and ANB are cofirst authors. HAC and CJLS are cosenior authors.

2. 2022 Maria Basil , Fabian Cardenas , Michael Morley , Justine Carl , Jeremy Katzen , Katherine Slovik , Apoorva Babu , Su Zhou , Madison Kremp , Katherine McCauley , Shanru Li , Xiaoming Liu , Rebecca Windmueller , Yun Ying , Kathleen Stewart , Michelle Oyster , Jason Christie , Joshua Diamond , John Engelhardt , Edward Cantu , Darrell Kotton , Jaymin Kathiriya , Alexis Brumwell , Joseph Planer , Shah Hussain , Steven Rowe , Harold Chapman, Edward Morrisey. The human distal respiratory airways contain a distinct multipotent secretory cell lineage that can regenerate lung alveoli. Nature, 2022.

3. 2022 Jaymin J. Kathiriya, Chaoqun Wang, Minqi Zhou, Alexis Brumwell, Monica Cassandras, Claude Le Saux, Max Cohen, Kostantinos-Dionysios Alysandratos, Bruce Wang, Paul Wolters, Michael Matthay, Darrell N. Kotton, Harold A Chapman, and Tien Peng. Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells. Nature Cell Biology, 2022.

4, 2020 Kathiriya JJ, Brummell AN, Jackson JR, Tang X, Chapman HA. Distinct Airway Epithelial Stem Cells Hide among Club Cells but Mobilize to Promote Alveolar Regeneration. Cell stem cell, 2020.

5. 2015 Andrew E. Vaughan, Alexis N. Brumwell, Ying Xi, Jeffrey E. Gotts, Doug G. Brownfield, Barbara Treulein, Kevin Tan, Victor Tan, Feng Chun Liu, Mark R. Looney, Michael A. Matthay, Jason R. Rock, Harold A. Chapman. Lineage-negative progenitors mobilize to regenerate lung epithelium after major injury. Nature, 2015.