Alyssa Ng
Los Angeles,CA
Summary
Highly skilled research associate with solid background in experimental design, data collection and analysis. Strengths include strong problem-solving abilities, effective communication skills and a deep understanding of research methodologies. I excel in problem-solving and data organization, contributing to impactful research outcomes and enhancing laboratory efficiency.
Overview
4
4
years of professional experience
Work History
Staff Research Associate
UCLA Health
Los Angeles, California
11.2023 - 07.2025
- Develop and optimize antibody expression vectors for monoclonal antibody production
- Perform transfection of plasmid constructs into mammalian cell lines for expression studies
- Conduct PCR for amplification and perform DNA assembly techniques
- Perform plasmid isolation, purification, and analysis to prepare vectors for downstream applications
- Utilize gel electrophoresis to verify the size and integrity of DNA, RNA, and protein samples
- Maintain cell culture for various mammalian cell lines, ensuring optimal conditions for cell growth and transfection
- Utilize CRISPR/Cas9 gene editing to introduce precise, targeted modifications in cell lines for functional studies or enhanced antibody production
- Conduct protein analysis using Western blotting to assess transfection and expression efficiency
- Execute cloning and subcloning of gene sequences into expression vectors, using restriction enzyme digestion and ligation techniques
Scientific Journal Biology Section Editor
California State University, Fullerton
01.2023 - 08.2023
- Edited the biology section of the 24th volume of the Dimensions, The Journal of Undergraduate Research in Natural Sciences and Mathematics at California State University, Fullerton
- Peer-reviewed and edited scientific papers of undergraduate biology researchers
- Collaborated with a team of 6 editors to efficiently publish the journal before the deadline
Undergraduate Student Researcher
California State University, Fullerton
Fullerton, California
08.2021 - 08.2023
- Worked directly under PI focusing on the purification of the manganese oxidizing protein, MopA-hp, found in Erythrobacter sp. SD-21
- Conducted bacterial cultures to induce protein expression of MopA-hp
- Utilized a French press for cell lysis, facilitating the extraction of the target protein from bacterial cells.
- Implemented gravity nickel affinity column chromatography and anion exchange chromatography for purification, optimizing conditions for maximum yield and purity.
- Collaborated with a team to analyze protein characteristics and ensure accurate results, contributing to the understanding of manganese oxidation processes.
- Partnered with PI to assist in writing grant proposals that generated funding for the lab
- Presented research at multiple conferences and symposiums
- Published paper to Dimensions Journal of Undergraduate Research
Education
Bachelor of Science - Biology, Molecular biology and Biotechnology
California State University, Fullerton
Fullerton, CA05-2023
Skills
- PCR, gel electrophoresis, DNA assembly
- Cell culture maintenance
- Protein purification
- Animal research certified
- Scientific report writing and presenting
- Critical thinking skills
- Team collaboration
- Data collection and organization
Timeline
Staff Research Associate
UCLA Health
11.2023 - 07.2025
Scientific Journal Biology Section Editor
California State University, Fullerton
01.2023 - 08.2023
Undergraduate Student Researcher
California State University, Fullerton
08.2021 - 08.2023
Bachelor of Science - Biology, Molecular biology and Biotechnology
California State University, Fullerton