Ambika Dudhate
Kansas City,KS
Summary
Researcher with experience in diverse environment to work with e.g: Clinical studies and molecular biology/computational analysis. Major qualities includes quick adaptation to new environment and to work in team. Always an enthusiast to learn new things and to gain new expertise.
Overview
4
4
years of professional experience
Work History
Senior Research Associate
University of Kansas Medical Center
11.2023 - Current
- As a Senior research associate at Immunology lab:
1. Performed advanced RNA sequencing (bulk and single-cell RNA-seq) analysis and bioinformatics workflows to derive meaningful insights into gene expression and molecular pathways.
2. Conducted wet lab experiments involving DNA, RNA, and protein-related techniques, ensuring precision and reproducibility in molecular biology research.
3. Managed laboratory operations, including supply ordering, inventory management, and equipment maintenance to support seamless research activities.
4. Oversaw mouse house facilities, coordinating animal care and experimental protocols while adhering to ethical and regulatory standards.
5. Analyzed, managed, and organized complex datasets, integrating computational and experimental findings.
6. Demonstrated exceptional organizational skills, quick learning, adaptability, and effective collaboration with peers to enhance lab productivity and research outcomes.
7.Manuscript writing and grant writing
Key Skills: RNA-seq (bulk & single-cell), bioinformatics, molecular biology, lab management, data analysis, mouse facility oversight, teamwork, adaptability.
Associate Scientist II
Stowers Institute For Medical Research
06.2022 - 10.2023
In my role as Associate Scientist II, I successfully carried out the following responsibilities at the institute:
1. Managed the processing of Next-Generation Sequencing (NGS) orders from various laboratories within the institute.
2. Optimized bioinformatics workflow for new single cell and Bulk RNA seq technologies.
2. Engaged with research team members to comprehend their research requirements, offering tailored solutions to address their specific challenges.
3. Optimized protocols for emerging NGS techniques, including tRNA sequencing, Ribosomal profiling, and Tn-seq, among others.
4. Delivered presentations and explanations on novel techniques introduced by different companies to keep research members informed about the latest developments in the NGS and genomics field.
5. Actively participated in meetings and conferences related to genomics, fostering connections within the sequencing community and staying abreast of the latest advancements in the field.
Post Doctor Fellow
University Of Kentucky
06.2020 - 01.2022
My Post Doctoral term with Dr.Markos Leggas, college of Pharmacy, University of Kentucky has given me extensive research experience in Clinical studies as follows:
1. Metagenomics and 16s rRNA analysis of infants with Neonatal Abstinence syndrome under treatment with Non-opiates drug Clonidine.
2. Determination of Transcriptomic signatures of Mithramycin and its Analogues (Mithramycin-SA-Trp and Mithramycin-SA-Phe-Trp) in Prostate cancer and Ewing sarcoma.
3. SNP genotyping to determine the correlation between the NAS severity and clinical outcomes.
4. Epigenetic analysis for profiling the genome wide methylome markers linked to NAS in newborn after prenatal exposure to opiates and other drug substances.
Education
Ph.D - Molecular Biology and Biotechnology
University of Tokyo
Master of Science - Plant Molecular biology and biotechnology
Tamil Nadu Agricultural University
Coimbatore, Tamil NaduSkills
- Scientific writing and publishing
- Mouse Handling and IACUC protocol management
- Laboratory management and data management
- Computer software handling
- Computer Language (R, Python, Linux)
- Experiments involving Protein, DNA And RNA, CRISPR-cas9
- Wet lab experiments (eg: handling Cell lines, processing stool samples)
- Data management and interpretation
- Data Analysis (Bulk RNA seq, Single cell RNA-seq,Spatial transcriptomics, Metagenomics, Chip-seq)
Accomplishments
List of Awards and Achievements:
1. Best poster award (2015): Secured Best poster award amongst 40 posters at 6th Indian scientist association in Japan held at Indian embassy, Tokyo while presenting my master’s research work on Functional analysis of defense related transcription factors in rice against sheath blight pathogen Rhizoctonia solani.
2. Best Presentation award (2016): Secured Best oral presentation in competition of 35 students from different countries at 3rd AIST international workshop and symposium held at Biomedical research institute, tsukubai science city, Japan presenting my PhD work on GWAS analysis in Arabidopsis thaliana for lateral root studies (not published).
3. Best Poster award (2018): Secured Best poster award amongst 25 posters at 2nd Asian natural environmental science center seminar held at The University of Tokyo, Tokyo while presenting my PhD’s research work on Genome-wide investigation of NAC transcription factor involved in abiotic stress response in Pearl millet.
4. Early Career research travel grant (2018): Secured the travel grant to be a speaker delegate to present my PhD work in front of scientist around the world at 6th plant genomics and gene editing congress held at Rotterdam Netherlands. Only two students (including me) were selected as early career researcher.
5. Second best presentation award (2018): Secured second place amongst 40 oral presentations at
International academic conference for graduate students held at Nanjing Agriculture University, Nanjing, China, while presenting my PhD’s research work on Elucidation of Drought tolerance in pearl millet by the help of OMICS technology.
Publications
1. Leggas, M., Dudhate, A., & Rohr, J. (2022). Selective targeting of aberrant ETS transcription factors using uniquely modified mithramycin analogs. Cancer Research, 82(12_Supplement), 1851-1851.
2. Govind, G., Kulkarni, J., Shinde, H., Dudhate, A., Srivastava, A., & Suprasanna, P. (2022). Plant abiotic stress tolerance on the transcriptomics atlas. In Advancements in Developing Abiotic Stress-Resilient Plants (pp. 193-236). CRC Press.
3. Yu, P., Shinde, H., Dudhate, A., Tsugama, D., Gupta, S. K., Liu, S., & Takano, T. (2021). Genome-wide investigation of SQUAMOSA promoter binding protein-like transcription factor family in pearl millet (Pennisetum glaucum (L) R. Br.). Plant Gene, 27, 100313.
4. Dudhate A, Shinde H, Yu P, Tsugama D, Gupta SK, Liu S; Takano T (2021). Comprehensive analysis of NAC transcription factor family uncovers drought and salinity stress response in pearl millet (Pennisetum glaucum). BMC Genomics 22:70 https://doi.org/10.1186/s12864-021-07382-y
5. Yu P, Shinde H, Dudhate A, Tsugama D, Gupta SK, Liu S; Takano T (2021). Genome-wide investigation of SQUAMOSA promoter binding protein-like transcription factor family in pearl millet (Pennisetum glaucum (L) R. Br.) (Under revision in plant gene journal)
6. Shinde H, Dudhate A, Lakshay Anand, Tsugama D, Gupta SK, Liu S and Takano T. (2020). Small RNA sequencing reveals role of pearl millet micro RNAs and their target genes in salinity stress. South African journal of Botany. Vol. no. 132. pp.395-402
7. Shinde H, Dudhate A, Tsugama D, Gupta SK, Liu S and Takano T. (2019). Pearl millet stress-responsive NAC transcription factor PgNAC21 enhances salinity stress tolerance in Arabidopsis. Plant Physiology and Biochemistry. Vol. no. 135. pp.546-553.
8. Shinde H, Tanaka K, Dudhate A, Tsugama D, Mine Y, Kamiya T, Gupta SK, Liu S and Takano T (2018). Comparative de novo transcriptomic profiling of the salinity stress responsiveness in contrasting pearl millet lines. Environmental and Experimental Botany. Vol. no. 155. pp. 619-627
9. Dudhate A, Shinde H, Tsugama D, Liu S, Takano T (2018) Transcriptomic analysis reveals the differentially expressed genes and pathways involved in drought tolerance in pearl millet [Pennisetum glaucum (L.) R. Br]. PLoS ONE 13(4): e0195908. https://doi.org/10.1371/journal.pone.0195908.
Timeline
Senior Research Associate
University of Kansas Medical Center
11.2023 - Current
Associate Scientist II
Stowers Institute For Medical Research
06.2022 - 10.2023
Post Doctor Fellow
University Of Kentucky
06.2020 - 01.2022
Master of Science - Plant Molecular biology and biotechnology
Tamil Nadu Agricultural University
Ph.D - Molecular Biology and Biotechnology
University of Tokyo
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