Amit Singh

Meticulous BSL-3 Lab Manager with proven performance in streamlining select agent and non-select agent protocols. Doctorate in Microbiology with studies in Advances in Microbiology. Postdoctoral Scientist with 4 years in fellowship at Albany Medical Center. Organized with research and presentations educating students, peers and superiors on research analysis. Published research findings through peer-reviewed articles and analyzed results tailoring to possible future applications.

• New York Academic of Science 2021-2022.
• Life time member of “Association of Microbiologist of India”. 2013- Life time member. Membership No. AMI/LN-305(7)2012.
• Life time member of “Indian Science Congress”. 2013- Life time member. Membership No. L21949.
• Life time member of “Society for advancement of agricultural sciences” 2013- life time member. Membership No. SAAS/018.

• Amit K. Singh, Xiuran Wang, and Wei Sun. Lung tissue-resident memory T cells induced by oral vaccination afford comprehensive protection against pneumonic Yersinia pestis infection. Mucosal Immunology (Submitted and under peer review).
• Amit K. Singh, Kathleen A. Stellrecht, Thilaka Arunachalam, Tarani K. Barman, Michael D. Robek, Michael J. Waxman, Sarah L. Elmendorf and Dennis W. Metzger (2021) Lack of replicating SARS-CoV-2 virus in a subset of PCR-positive COVID-19 congregate care patient samples. Journal of Clinical Virology (Accepted).
• Xiuran Wang, Peng Li, Amit K. Singh, Ziqiang Guan, Roy Curtiss 3rd and Wei Sun (2021) Remodeling Yersinia pseudotuberculosis to generate a highly immunogenic outer membrane vesicle vaccine against pneumonic plague. PNAS (submitted and under peer review).
• Wang X, Singh AK, Sun W (2020). Protection and Safety Evaluation of Live Constructions Derived from the Pgm− and pPCP1− Yersinia pestis Strain. Vaccines, 8(1), 95.
• Wang X, Singh AK, Zhang X, Sun W (2020). Induction of Protective Antiplague Immune Responses by Self-Adjuvanting Bionanoparticles Derived from Engineered Yersinia pestis. Infection and Immunity, 88(5).
• Singh AK, Wang X, Sun W (2020). Oral vaccination with live attenuated Yersinia pseudotuberculosis strains delivering a FliC180-LcrV fusion antigen confers protection against pulmonary Y. pestis infection. Vaccine.
• Singh AK, Curtiss R, Sun W (2019). A Recombinant Attenuated Yersinia pseudotuberculosis Vaccine Delivering a Y. pestis YopENt138-LcrV Fusion Elicits Broad Protection against Plague and Yersiniosis in Mice. Infection and immunity, 87(10), e00296-19.
• Sun W, Singh AK. (2019). Plague vaccine: recent progress and prospects. npj Vaccines, 4(1), 1-9.
• Ganesh TMLN, Singh AK, Balakrishna K, Murali HS, Batra HV (2015). Immunogenicity and protective efficacy of Brucella abortus cocktail recombinant protein (rO + rP) against lethal challenge of B. abortus 544 and B. melitensis 16M in murine model. Molecular Immunology 71: 34-41.
• Singh AK, Kingston JJ, Gupta SK, Batra HV (2015). Immunization with recombinant rVE elicited CD4+ and CD8+ T cell mediated long-term memory immune responses and protection in mouse model. Frontiers in Microbiology 6: 1407- DOI: 10.3389/fmicb.2015.01407.
• Lohith GK, Singh AK, Kingston JJ, Murali HS, Batra HV (2015). Evaluation of recombinant leukocidin domain of VvhA exotoxin of Vibrio vulnificus as an effective toxoid in mouse model. Immunology letters 167: 47-53.
• Singh AK, Kingston JJ, Murali HS and Batra HV (2014). A recombinant bivalent fusion protein rVE confers active and passive immunogenic protection against Yersinia enterocolitica infection in mice. Vaccine 32(11) 1233-1239.
• Vrushabhendrappa, Singh AK, Balakrishna K, Murali HS, Batra HV (2014). Studies on recombinant glucokinase (r-glk) protein of Brucella abortus as a candidate vaccine molecule for brucellosis. Vaccine 32(43): 5600-5606.
• Singh AP, Singh R, Singh AK, Gupta AK and Raina VS (2013). Influence of microclimate modification on sexual behaviour and semen characteristics of Murrah buffalo bull during hot humid period in India. Indian Journal of Animal Sciences 83 (4): 431-434.
• Singh VK, Singh AK, Kumar R and Atreja SK (2013). Development of soya milk extender for semen cryopreservation of Karan Fries (Crossbreed Cattle). CryoLetters 34(1): 52-61.
• Singh VK, Atreja SK, Kumar R, Chhillar S and Singh AK (2012). Assessment of intracellular Ca++, cAMP and 1,2-Diacylglycerol in cryopreserved Buffalo (Bubalus bubalis) spermatozoa on supplementation of Taurine and Trehlose in the extender. Reprod Domest Anim 47(4): 587- 590.

§ Singh AK, Singh VK, Narwade BM, Mohanty TK and Atreja SK (2012). Comparative quality assessment of buffalo (Bubalus bubalis) semen preserved in egg-yolk and soya based extenders at refrigerated temperature (5˚C). Reprod Domest Anim 47(4):596-600.

• Generation of single chain antibody fragment (rScFv-TE 241) for immune based detection of staphylococcal enterotoxin B. Shylaja Ramlal, Bhairab Mondal, Amit Kumar Singh, Radhika Madam Urs, HV Batra, Monmohan Parida. (Patent Submitted)
• A recombinant chimeric rOP protein for effective protection against pathogenic Brucella species. Ganesh Babu TMLN, Amit Kumar Singh, Harishchandra Sripathi Murali, Harsh Vardhan Batra (Patent Filed: 2723/DEL/2015).
• A trivalent fusion protein rVECTXb. Amit Kumar Singh, Joseph Jeyabalaji Kingston, Kunal Singh, Harsh Vardhan Batra (Patent Filed: 201711013643).
• A recombinant fusion polypeptide rVECPEC for use as a vaccine. Amit Kumar Singh, Joseph Jeyabalaji Kingston, Kunal Singh, Harsh Vardhan Batra (Patent Filed: 201711013644).

• Book chapter entitled, “Methane mitigation strategies by altering ruminants enteric micro-flora”, published in Environmental Pollution and Management. ISBN 978-93-5196-308-0.
• Book chapter entitled, “Chapter-4 Mycology”, published in Medical Microbiology. ISBN 978-3-11-051764-4.
• Article on Molecular basis of Metastasis, published in Livestock line September 2012.

• ASM-travel award 2018
• SERB-DST NPDF 2016
• ICAR-NET 2013
• Joint CSIR-UGC NET JRF 2011 with AIR 35th,
• GATE (2011-All India Rank 723, 98.7 percentile),
• British IELTS examination (2009; score 6.5 bands),
• GATE (2007- All India Rank 1325, 96.7 percentile).