Graph Pad
Amy L Lynne Confides
Lexington,Ky
Summary
Laboratory-trained Scientist offering over 20 years working in Biochemisty and Molecular Biology. Experience in managing laboratory experiments, equipment and personnel. Well-versed in developing experiment protocols and reporting findings. Strong work ethic, adaptability and interpersonal skills. Looking for Scientist position.
Overview
28
28
years of professional experience
Work History
Scienctist II
University of Kentucky, College of Health Sciences
01.2006 - 01.2024
- Organization of Samples, Chemicals, Supplies, Protocols, Ensuring adequate Safety Conditions following Hazardous Waste and Biosafety
- Performed a multitude of Experiments involving Biochemistry, Immunology, Molecular, Genetic and Cellular Biology.
- The labs focus was on Skeletal Muscle and adipose tissue and Aging
- Created and maintained Spreadsheets of all specimins collected. Animal models, tissue culture and Human muscle and fat biopsies were used for experimitation.
- Trained undergraduate and graduate students on a variety of Laboratory experiments.
- Designed custom assays for the detection of specific enzymes and mRNA
- Prepared reports, presentations, and manuscripts for external stakeholders.
- Performed statistical analyses using software such as GraphPad and Sigma Stat.
- Provided training on laboratory safety procedures and proper usage of lab equipment as needed.
- Prepared reports and presentations.
- Analyzed and interpreted data from laboratory tests for scientific publications.
- Supervised junior researchers and provided guidance on projects.
- Managed laboratory inventory and ordered necessary supplies as needed.
- Maintained detailed records of experiments, results, and conclusions.
- Developed standard operating procedures (SOPs) for various laboratory experiments.
- Monitored laboratory equipment performance, set up meetings with professionals that performed needed repairs, calibrations, and preventative maintenance.
- Participated in weekly team meetings to discuss progress, present raw data and graphs and collaborate on solutions to any problems.
Sr. Lab Technician
University of Kentucky, College of Medicine
Lexington, KY
10.2000 - 01.2006
- Performed quality control tests.
- Operated laboratory equipment and tools according to regulations and instructions.
- Trained laboratory employees in lab techniques and procedures of lab protocols.
- Utilized computer software for data entry, management, and analysis.
- Presented experimental findings in presentations with graphs, charts and other visual aids.
- Prepared buffers, reagents, stock solutions and other elements of chemical analysis as appropriate in support of operations by other laboratory analysts.
- Operated autoclave to facilitate chemical reactions in glassware or plasticware, recording results of procedures performed on biological samples or reagents.
- Participated in inventory record-keeping and transfer or receipt of new inventory of biological samples.
- Maintained detailed records of all experiments conducted in the lab.
- Coordinated with research team to ensure proper data collection and analysis techniques.
- Verified accuracy of data entered into laboratory information systems.
- Provided guidance to junior technicians on laboratory procedures and protocols.
- Analyzed experimental data to identify trends or outliers that require further investigation.
- Cleaned and sanitized sensitive laboratory areas, following standard procedures to protect against sample contamination and facilitate accurate test results.
- Created detailed records of experiment setup parameters and environmental conditions.
- Ensured compliance with all applicable regulations related to laboratory operations.
- Placed orders for laboratory supplies, equipment or chemicals in keeping with laboratory needs or standard schedule for replacement of perishable inventory.
Lab Technician
University of Kentucky
01.1996 - 01.1998
- Collected, labeled and stored lab samples according to laboratory specifications.
- Disposed of laboratory materials and samples using well established guidelines and instructions.
- Used time management and adaptability skills to prioritize and run multiple tests.
- Collected and organized data to perform data analysis and prepare technical reports.
- Attended lab meetings to participate in research discussions.
- Monitored experiments to identify potential issues or anomalies.
Education
Master of Science MS - Medical Science
01.2009
Bachelor of Science BS - Biology
01.1996
Skills
- Spectroscopy Techniques
- Cell Culture Techniques
- IHC/ Immunohistochemistry
- RNA, DNA & Protein Purification
- Histochemistry
- Quantitative skills
- ELISA Assays
- Safety processes and procedures
- RealTime PCR
- Western Blotting
- Research management
- Data Analytics
- Microscopy
- Experiment protocol development
- Health and Safety Regulation
- Sectioning Tissue (Cryostat)
- Teamwork and Collaboration
- TUNEL assays
- Immunoprecipitaiton
- Adaptability and Flexibility
- Knowledgeable in Micro RNA and mRNA analysis
- Analytical Thinking
- Immunocytochemistry
- Molecular Bench Work
- FISH (Fluorescent In Situ Hybridization)
- Genotyping
- Stem Cell Studies
- Recordkeeping
- Routine Laboratory Skills
Accomplishments
- SKILLS Laboratory Management: Laboratory Organization of Samples, Chemicals, Supplies, Protocols, and Data, Ensuring adequate Safety Conditions following Hazardous waste and Biosafety Protocol, Ordering Supplies, Training Undergraduate and Graduate Students
- Strong background in Biochemistry, specifically, Immunology, Molecular, Genetic and Cellular Biology with a focus on Skeletal Muscle using both Basic and Translational Sciences
- Laboratory techniques: In tissue culture, whole animal, and human laboratory experiments
- Immuno-histochemistry, Microscopy, DNA, RNA, and Protein Extraction and Purification, Tissue Culture of Immortalized Cell Lines and Primary Cells, Primary Cell Isolation, Maintaining Cell Lines; Proliferation, Subculturing, and Freezing Down Cells, Immunocytochemistry and Transient Transfections, Cloning Plasmid DNA, Transformation and Purification of Plasmid DNA, Agarose Gel Electrophoresis, Endpoint, Reverse Transcriptase and Real-Time PCR, Western Blotting, Acrylamide Gel Electrophoresis, ELISA Assays, Enzymatic Assays, Protein-Protein and Protein-RNA Immunoprecipitation, RNA-RNA and RNA-Protein fluorescent in situ hybridization Assay Development, Creating New or updated Protocols, Statistical analysis using Sigma Plot and GraphPad, Data Presentation, Knowledge of Lab Archives, Redcap and IRB Renewals
Publications
· Author in 26 peer-reviewed published papers and 72 Abstracts.
· Developed and wrote New or updated Protocols
· Used OneNote and Lab Archives for lab notes
· Worked with Redcap and IRB Renewals
- I used Microsoft Excel to develop inventory tracking spreadsheets.
Interests
Professional Affiliations: , 2010-present University of Kentucky Center for Muscle Biology 2008-Present University of Kentucky Center for Clinical and Translational Studies
Additional Information
- HONORS AND AWARDS , 2010 Employee of the Year Award University of Kentucky, College of Health Sciences 2010 Second Place Poster, Physiology 50th Anniversary University of Kentucky
Software
Sigma Plot
Zen (Zeiss Microscope)
Word
PowerPoint
Excel
One Note
Lab Archives (UK)
Adobe
Image Lab Software (Bio-Rad ChemiDoc Imager)
Certification
· Hazardous Waste General
Chemical Hygiene
· Bloodborne Pathogens
· Shipping Dangerous Goods
· Autoclave,
Fire Extinguisher
· RCR Responsible Conduct of Research Training Basic
RCR in-Person Training,
· CITI Collaborative Institutional Training Initiative
· CITI Modules Completed: History and Ethical Principles, Research and HIPAA Privacy Disclosure, COVID-19 Informed Consent, Populations in Research Requiring Additional Considerations or Protections, Regulations and Process, FDA Regulated Research
Timeline
Scienctist II - University of Kentucky, College of Health Sciences
01.2006 - 01.2024
Sr. Lab Technician - University of Kentucky, College of Medicine
10.2000 - 01.2006
Lab Technician - University of Kentucky
01.1996 - 01.1998
University of Kentucky - Master of Science MS, Medical Science
University of Kentucky - Bachelor of Science BS, Biology
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