Ana Belen Puga Garcia
Gainesville,FL
Summary
Embryologist Analytical and committed professional with 13 years of experience in assisted reproduction including 8 years in human reproduction and 5 years in bovine reproduction. Committed to continuous improvement and lifelong learning, I seek to contribute to the success of the clinic by leveraging my technical experience and leadership skills to achieve outstanding results.
Overview
13
13
years of professional experience
Work History
Senior Embryologist
Vytelle
Gainesville, Florida
02.2022 - Current
- Maintain flexibility for working hours as IVF processes are time-dependent, includes some weekend work
- Follow established IVF processes including plate preparation; oocyte collection and maturation; semen processing; embryo culture, evaluation, loading and freezing
- Lead a team of embryologists, lab assistants, and management professionals in a dynamic and growing environment
- Review and correct the work of technicians to ensure compliance with SOPs, providing constructive feedback
- Contribute to the training department by continually improving new technician training and updating training materials
- Instruct staff on cleaning SOPs and verify their proper execution to maintain high hygiene standards
- Supervise quality control of the lab and equipment, ensuring optimal functionality and compliance with specifications
- Ensure the integrity and traceability of media, semen collection, and embryo production data
- Collaborate with the production team to analyze results and optimize protocols and procedures
- Prepare and present detailed reports on lab performance, identifying trends and areas for improvement
- Maintain availability to travel and provide technical support, training, or supervision at other labs
- Assist technicians in resolving operational issues, providing quick and effective solutions
- Participate in specific tests to improve lab results and effectiveness
- Provide technical support and troubleshooting in other labs, ensuring a consistent operational standard
- Observe and suggest improvements in logistics and scheduling during visits to other labs to optimize efficiency
- Collaborate with veterinarians and administrators to provide superior customer service as a representative of Vytelle.
Laboratory Embryology Specialist
Cryogen
Guadalajara
06.2017 - 05.2021
- Prepared maturation medium and actively participated in oocyte retrieval and sorting, both in the field and laboratory
- Proficiently prepared fertilization and culture media and executed semen manipulation for FIV
- Expertly conducted oocyte fertilization, denudation, zygote culture, and quality control
- Managed the embryo and cryopreserved cell bank, including classification, packaging, embryo freezing, and vitrification
- Performed skin biopsies (from animals to be cloned), cell culture, cell freezing, follicular aspiration from slaughterhouse ovaries, and in vitro maturation of slaughterhouse oocytes
- Mastered techniques like enucleation of oocytes, donor cell transfer, fusion, activation, and aspiration
- Played a key role in improving the performance of trophoderm biopsy
- Collected semen samples and interpreted results using the IVOS II equipment
- Prepared and diluted samples for freezing using the IMV equipment
- Ensured laboratory quality control and managed the semen bank.
Embryology Specialist
Valencia Institute of Infertility (IVINSEMER)
Guadalajara
04.2015 - 05.2017
- Oversaw quality evaluation and control in the FIV laboratory, ensuring the application of protocols and procedures among laboratory personnel
- Managed various aspects of FIV procedures, including human oocyte identification, evaluation in follicular fluid, embryo culture, classification, selection, and transfer, conventional FIV and ICSI, micromanipulation, gamete and embryo vitrification, and assisted hatching with OCTAX and Saturn lasers
- Achieved a 99% survival rate in cryopreserving oocytes and embryos
- Conducted in vitro maturation of oocytes (IVM) and excelled in the implementation of Cryotech and Cryotop vitrification methods
- Maintained records and reported results to authorities to ensure laboratory performance
- Specialized in Piezo, ICSI, and Cryotech/Vitrification Kuwayama method, receiving personal training from Dr
- Kuwayama, the creator of the Cryotech brand
- Provided professional training to laboratory staff, including training in vitrification, FIV cycle management, and database maintenance for donor banks.
Embryology Specialist
Mexican Institute of Infertility (IMI)
Guadalajara
02.2012 - 04.2015
- Developed standardized protocols, conducted quality assessments, and ensured quality control
- Supervised and routinely trained four technicians, ensuring peak performance in laboratory procedures
- Successfully managed embryology and andrology laboratories
- Achieved a 98% survival rate in cryopreserving oocytes and embryos
- Prepared ovarian tissue for cryopreservation and in vitro activation (IVA)
- Supervised and implemented various FIV laboratory procedures, including human oocyte identification, evaluation in follicular fluid, embryo culture, classification, selection, and transfer, conventional FIV and ICSI, micromanipulation, gamete and embryo vitrification, and laser or PIEZO-assisted hatching
- Expertise in Piezo, ICSI, and Cryotech/Vitrification Kuwayama method
- Conducted professional development training for staff members, including vitrification, FIV cycle management, and laboratory management.
Andrology/Embryology Laboratory Technician
Institute of Reproductive Medicine (IMER)
Guadalajara
01.2011 - 04.2015
- Received training in low-complexity assisted reproduction techniques, including intrauterine insemination (IUI), as well as medium-complexity techniques such as in vitro fertilization (IVF)
- Conducted semen analysis and prepared samples for IUI and IVF procedures
- Proficiently performed in vitro fertilization and intracytoplasmic sperm injection (ICSI)
- Expertly identified and evaluated human oocytes in follicular fluid, conducted embryo culture, and executed complete classification/selection and embryo transfer, both conventional FIV and ICSI
- Assumed responsibility for quality control and evaluation of laboratory procedures.
Education
Served as a speaker in fixed-time artificial insemination courses conducted by the Cryogenic -
01.2021
the Agricultural Training Center of Mexico -
08.2020
Embryology certification from the American Society for Reproductive Medicine -
12.2016
Achieved certification as a trophoderm biopsy technician -
IGENOMIX
01.2016
Bachelor’s degree - biology
University of Guadalajara
11.2011
Diploma - Assisted Reproduction Techniques
Earned certification in "Training in Media Handling for -
National Institute of Agricultural Technology (INTA)
Skills
- Excellent manual dexterity and visual acuity
- Excellent attention to detail
- Excellent analytical and problem-solving skills
- Driven by quality
- Programming proficiency
- Organized
- Process Development
- Team Collaboration
Languages
- Spanish
- English
Additional Information
- Certified in the workshop on vitrification of human oocytes and embryos in November 2015, led by Dr. Kuwayama, the creator of the Cryotech brand. Attended a workshop on ovarian tissue vitrification on March 14, 2015, in Guadalajara, Jalisco. Completed PIEZO-ICSI training in March 2015 under Kenichiro Hiraoka. Participated in the workshop of the Latin American Assisted Reproduction Network, "Advances, Evidence," in November 2014.
Languages
Spanish
Professional
English
Elementary
Timeline
Senior Embryologist
Vytelle
02.2022 - Current
Laboratory Embryology Specialist
Cryogen
06.2017 - 05.2021
Embryology Specialist
Valencia Institute of Infertility (IVINSEMER)
04.2015 - 05.2017
Embryology Specialist
Mexican Institute of Infertility (IMI)
02.2012 - 04.2015
Andrology/Embryology Laboratory Technician
Institute of Reproductive Medicine (IMER)
01.2011 - 04.2015
Served as a speaker in fixed-time artificial insemination courses conducted by the Cryogenic -
the Agricultural Training Center of Mexico -
Embryology certification from the American Society for Reproductive Medicine -
Achieved certification as a trophoderm biopsy technician -
IGENOMIX
Bachelor’s degree - biology
University of Guadalajara
Diploma - Assisted Reproduction Techniques
Earned certification in "Training in Media Handling for -
National Institute of Agricultural Technology (INTA)
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