Angel David Nunez Correa

Longterm-Covid Patient Serum Experiments on Peripheral Sympathetic and Sensory Neurons

• Performed extraction and dissociation of primary rodent sensory and sympathetic ganglia neurons followed by culture in 96-well plates.
• Prepared experiments for high-throughput automated imaging instrumentation, Opera Phenix.
• Managed laboratory rat colonies, ensuring compliance with research and animal welfare protocols while supporting experimental needs
• Updated laboratory protocols to ensure accuracy and compliance.
• Maintained laboratory equipment and supplies, facilitating uninterrupted research operations.
• Assisted in developing and implementing experimental protocols, enhancing research efficiency and consistency.

Acute Pharmacological Augmentation of Kv7 K+ Ion Channel to Prevent Post-Traumatic Epilepsy and Chronic Traumatic Encephalopathy

• Performed various single-cell calcium imaging experiments.
• Conducted confocal imaging in study of antiepileptic drugs on TBI to contribute to understanding treatment effects.
• Performed genotyping to determine genetic traits in mouse populations.
• Maintained mammalian cell lines under sterile techniques to support experimental integrity.
• Prepared reagents and solutions following standard laboratory formulas and procedures.
• Troubleshot and resolved equipment and process issues.
• Collaborated with researchers and scientists to design and execute experiments.

• Evaluated student performance through assessments, ensuring consistent application of grading standards.
• Assisted in developing and delivering engaging course materials for undergraduate students.
• Identified, neutralized, documented, different nucleic acids, culture samples, and hazardous chemical materials in accordance with CDC, NIH and University bio-safety protocols.

• Research Project: Bioinformatic Analysis of Attempted Cloning and Recombinant Protein Expression of δ-latroinsectotoxin, a Putative Insect-specific Protein Toxin Produced by the Brown Widow Spider, Latrodectus geometricus.