ANISHA PRASAD
Pittsburgh,PA
Summary
Experienced researcher with a strong background in molecular biology and protein engineering. Organized professional with ability to work in fast paced environments and adapt to new situations. Reliable team player with very good communication skills. Passionate about applying the knowledge in the field to contribute to healthcare.
Overview
6
6
years of professional experience
Work History
Research Associate
NOVASENTA
11.2022 - Current
- Established small-scale protein expression and purification and QC methods in-house
- Collaborated across teams to generate antibody kinetics data using Gator that enabled decision making on antibody hits
- Worked on ELISA assay development for pharmacokinetic analysis of lead molecules
- Trained on lentivirus production and basics of flow cytometry
- Played a major role in implementation of LabGuru ELN for the company
Associate Scientist
NEUBASE THERAPEUTICS, INC
07.2021 - 10.2022
- Performed in vitro screening assays to test the efficacy of PNA drugs and worked on optimizing qPCR assay to study the role of PNAs
- Established CHO cell culture in-house and optimized a protocol for transfection and purification of protein from CHO cells
- Collaborated with team members to support KRAS, DM1 and gene editing programs
- Regularly participated in research meetings and presented data to superiors and peers
- Performed data analysis, interpretation of results and recorded data consistently in electronic notebook system (Benchling)
- Performed inventory and ordered supplies for the biology team
Research Associate
MOLECULAR TEMPLATES, INC
07.2019 - 06.2021
- As an integral member of the R&D team performed protein production/purification, protein assays, cloning experiments and data analysis, contributing to a variety of projects
- Provided inputs to optimize and improve experimental protocols, trained technicians and new additions to the team, monitored laboratory supplies, inventory, and ordering.
Protein Engineering Summer Intern
CYTOMX THERAPEUTICS
05.2018 - 07.2018
- Developed a TaqMan based qPCR assay to quantify the number of lentiviral plasmids integrated into the genome of a transduced cell line and helped the team with several different DNA cloning projects
- Contributed to a study of CDR mutations in antibodies that allowed the use of techniques like transfection and protein purification from mammalian cells
Education
Master of Science - Cell and Molecular Biology
THE UNIVERSITY OF TEXAS
Dallas, TXBachelor of Engineering - Biotechnology
RAMAIAH INSTITUTE OF TECHNOLOGY
Bengaluru, IndiaSkills
- DNA, RNA extraction
- Molecular cloning
- PCR, Gel electrophoresis, qPCR
- Protein purification by affinity chromatography
- Western Blot - traditional and automated (Jess)
- Cell culture, transformation, transfection
- Lentivirus transduction
- ELISA
- Data analysis (GraphPad Prism)
- ELN: Benchling and LabGuru
Accomplishments
Recognized as 'Excellence award winner' two times at Novasenta for working on generating valuable antibody kinetics data using Gator Biolayer Interferometry and for implementation of a new electronic notebook system for the company and training members on how to use it.
Timeline
Research Associate
NOVASENTA
11.2022 - Current
Associate Scientist
NEUBASE THERAPEUTICS, INC
07.2021 - 10.2022
Research Associate
MOLECULAR TEMPLATES, INC
07.2019 - 06.2021
Protein Engineering Summer Intern
CYTOMX THERAPEUTICS
05.2018 - 07.2018
Master of Science - Cell and Molecular Biology
THE UNIVERSITY OF TEXAS
Bachelor of Engineering - Biotechnology
RAMAIAH INSTITUTE OF TECHNOLOGY