Anjali Gupta

Skills: Project ideation, Skin and hair biology, Assay development, Scientific communication, Program management, CRO management, High throughput screening, AI based drug discovery, Molecular and cell biology, Biophysics, Topical molecule safety, Strategic planning, Stakeholder engagement, Team leadership, Cross-functional collaboration

• Conceived scientific focus and designed novel projects for a brand.
• Lead team of scientists and associates to execute high-impact research projects with translatable potential to develop novel products for beauty industry.
• Developed high throughput molecule discovery workflows for molecule discovery.
• Managed scientific projects fostering cross-functional collaborations and CRO management.
• Strategic planning to facilitate bench science translation to market.

Skills: Project ideation, Skin and hair biology, Assay development, Scientific communication, High throughput screening, AI based drug discovery, Molecular and cell biology, Biophysics, Topical molecule safety, Cross-functional collaboration,

• Conceived and designed novel projects relevant to skin and hair biology.
• Developed high throughput molecule discovery workflows for molecule discovery.
• Performed assay development (biochemical, biophysical and cell biology) and screening for molecule discovery.
• Lab management and development to establish biotech research facility.
• Strategic planning to facilitate bench science translation to market.

Project: Role of sphingosine-1-phosphate signaling in the maintenance of meningeal lymphatic vessel structure and function.

Skills: Mouse genetics, Murine tissue processing, Mouse brain surgery using stereotax, Primary cell cultures, Lipid bioanalysis, Confocal microscopy, Flow cytometry, Transcriptomics (scRNA-seq), ImageJ, FlowJo, R, Python.

• Gained experience in GPCR lipid signaling, cerebrovascular biology, apolipoprotein biology, and bioactive lipid signaling.
• Manipulated sphingolipids in genetic mouse models to study meningeal vasculature on intact skull and designed assay to measure lymphatic drainage in-vivo.
• Performed immune cell analysis using flow cytometry and analyze meningeal transcriptomes using scRNA-seq.
• Awarded American Heart Association funding for 2 years (extendible) as principal investigator (USD 141K).
• Awarded American Federation for Aging Research funding for 1 year as principal investigator (USD 60K).

Dissertation: Probing into cell-membrane protein interaction of Helicobacter pylori TlyA.

Skills: Protein biochemistry (protein purification and functional characterization), Biophysical characterization (UV-Vis), Circular dichroism, Surface plasmon resonance (SPR), Molecular biology

• Cloned and recombinantly expressed a putative Helicobacter pylori hemolysin gene in E. coli.
• Purified hemolysin expressed in E. coli. Performed biochemical and biophysical characterization using assays such as SPR and RBC lysis.

Dissertation: Role of membrane lipid composition in plasma membrane dynamics, organization, and protein function.

Skills: TIRF microscopy, Fluorescence spectroscopy based microscopy (FCS, FLIM, FRET), Mass spectrometry based lipidomics (LC-MS/MS), Hydrogen deuterium exchange mass spectrometry (HDXMS), Atomic force microscopy (AFM), Mammalian cell culture, Artificial lipid membranes, Membrane protein reconstitution, R, ImageJ, Imaris, Agilent Mass Hunter, DynamX

• Performed cell-based assays to study membrane dynamics (TIRF-FCS, FLIM) and correlated that with membrane lipid composition as analyzed by LC-MS/MS and HDXMS.
• Performed FRET-FCS and TIRF-FCS assays to probe the membrane interaction mechanism of dengue virus and IDPs.
• Published 12 research papers in internationally recognized peer-reviewed journals.
• Established collaborations to integrate state-of-the-art technologies (imaging FCS with lipidomics, HDXMS and FLIM).
• Served as key personnel in a joint NUS-Humboldt University (HU) at Berlin grant (SGD 60K).
• Received Student Research Achievement Award at Annual Meeting of the Biophysical Society 2019.