Developing and implementing solutions to challenging material, chemical, biophysical, and biochemical research problems. Experience in Carbon-based materials (CNT, Graphene, and GQDs) and other 2D nanomaterials (MoS2) exfoliation and their use in battery, supercapacitor, and energy-related applications. Experience in design, synthesis, and analysis of protein conjugates, protein-nucleic acid nanocomplexes, medicinal molecules, fluorescent molecular probes, Enzyme kinetics, and biocatalysis for pharmaceutical, biomedical applications.
Project: Engineered Protein-RNA nanoparticles for effective long-distance transport, delivery, and gene silencing in plants
GMP, GLP in Laboratory Testing & Validation
undefined* Analytical Assays: Protein purification and analytical characterization by SEC, Analytical gel Filtration (GFC), HIC, AKTA, RP-HPLC, UPLC, Agarose & SDS-PAGE electrophoresis, Enzyme kinetics & assay development
* Biophysical Characterization: Circular dichroism (CD), UV-Vis & Fluorescence Spectroscopy, TGA, DSC, Zeta Potential, Dynamic light scattering (DLS)
* Organic synthesis: Multi-step organic reactions, Protein conjugation, Protein-nanocomplexes synthesis, Thin-layer Chromatography (TLC), Column chromatography, 1D NMR (Nuclear magnetic resonance), Mass spectrometry, UV-Visible, FT-IR spectroscopy
* Nanomaterials exfoliation and characterization: Exfoliation of 1D and 2D nanomaterials by proteins, Raman spectroscopy, Electron microscopy (TEM, SEM), X-ray diffraction, Thermal and electrical conductivity studies
* Electrochemistry techniques: Cyclic voltammetry (Pentiostat), Galvanostatic charge/discharge, Electrochemical impedance (EIS) spectroscopy
* Software's: NAMD/VMD simulation, Chem draw, Chemstation, Origin Pro, KaleidaGraph, MS office
1. Mansi, M., Megan, P., Kalluri, A., Kumar, C. Adsorption of metal ions on graphene sheet for applications in environmental sensing and wastewater treatment. Sensors and Actuators Reports, 2022, 4, 100077. DOI: 10.1016/j.snr.2022.100077
2. Basu, S., Bhukya. V.N., Kalluri, A., Patra, P., Sarkar, J. Self-assembly of graphene nanoparticles on biocompatible polymer through dewetting. Surfaces and Interfaces, 2021, 23, 101009. DOI: 10.1016/j.surfin.2021.101009
3. Tianjian Yang, Kyle Benson, Hailin Fu, Tianrui Xue, Ziyuan Song, Hanyi Duan, Hongwei Xia, Ankarao Kalluri, Jie He, Jianjun Cheng, Jianjun Cheng, Challa V. Kumar, and Yao Lin. Modeling and designing particle-regulated amyloid-like assembly of synthetic polypeptides in aqueous solution.
Biomacromolecules 2022, 23, 1, 196–209. DOI: 10.1021/acs.biomac.1c01230
4. Basu, S., Bhukya. V.N., Kalluri, A., Patra, P., Sarkar, J. Dewetting assisted self-assembly of graphene nanoparticles by diverse approaches. Bulletin of Materials Science, 2021, 44, 246. DOI: 10.1007/s12034-021-02535-x
5. Rawat, S., Jain, K.G., Gupta, D., Raghav, P.K., Chaudhuri, R., Shakeel, A., Arora, V., Sharma, H., Debnath, D., Kalluri, A., Agrawal, A., Jassal, M., Dinda, M., Patra, P., Mohanty, S. Graphene nanofiber composites for enhanced neuronal differentiation of human mesenchymal stem cells. Nanomedicine, 2021, 16 (22), 1963-1982. DOI: 10.2217/nnm-2021-0121
6. Jeong, S., Pinals, R.L., Dharmadhikari, B., Song, H., Kalluri, A., Debnath, D., Wu, Q., Ham, M.H., Patra, P., Landry, M.P. Graphene quantum dot oxidation governs noncovalent biopolymer adsorption. Sci Rep, 2020, 10, 7074. DOI: 10.1038/s41598-020-63769-z
7. Kalluri, A., Megan, P., Mansi, M., Kumar, C. Exfoliated and water-dispersible biocarbon nanotubes for enzymology applications. Methods in Enzymology, 2020, 630, 407-430.
DOI: 10.1016/bs.mie.2019.11.010.
8. Kalluri, A., Debnath, D., Dharmadhikari, B., Patra, P. Graphene quantum dots: Synthesis and applications. Methods in Enzymology, 2018, 609, 335-354. DOI: 10.1016/bs.mie.2018.07.002
9. Kokal, R., Melepurath, D., Kalluri, A., Singh, S., Macwan, I., Patra, P., Gilrade, J. Solar cells with PbS quantum dot sensitized TiO2–multiwalled carbon nanotube composites, sulfide-titania gel, and tin sulfide coated C-fabric. Phys. Chem. Chem. Phys. 2017, 19, 26330-26345. DOI: 10.1039/C7CP05582J
10. Kolay, A., Kokal, R., Kalluri, A., Macwan, I., Patra, P., Partha, G., Melepurath, D. New antimony selenide/nickel oxide photocathode boosts the efficiency of graphene quantum dots co-sensitized solar cell. ACS Applied Materials and Interface, 2017, 9, 34915–34926.
DOI: 10.1021/acsami.7b09754.
11. Xing, J., Singh, S., Zhao, Y., Duan, Y., Guo, H., Hu, C., Ma, A., George, R., James, Z., Xing, A., Kalluri, A., Macwan, I., Patra, P., Chen, J. Increasing vaccine production using pulsed ultrasound waves. PLoS One, 2017. DOI: 10.1371/journal.pone.0187048.
2020 Accelerate UConn Program
2021-2022 Graduate Research Assistant Fellowship, UConn, CT, USA
2018-2020 Graduate Teaching Assistant Fellowship, UConn, CT, USA
2016-2017 Graduate Research Assistant fellowship, University of Bridgeport, CT, USA
2017 Membership and excellence award by the Honor Society of Phi Kappa Phi.
2017 Best student service award in Department of Biomedical Engineering, University of Bridgeport, USA.
2012-2014 Research fellowship awarded by National Institute of Pharmaceutical Education & Research (NIPER), Raebareli, UP, India
LinkedIn: https://www.linkedin.com/in/ankarao-kalluri-35679411b/
Google scholar: https://scholar.google.com/citations?user=PNNNWIYAAAAJ&hl=en
Research Gate: https://www.researchgate.net/profile/Ankarao_Kalluri