Summary
Overview
Work History
Education
Skills
Accomplishments
Additional Information
Work Availability
Publications
Awards and Fellowships
Research weblinks
Timeline
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Ankarao KALLURI

Ankarao KALLURI

Material & Protein Engineer | Analytical & Medicinal Chemist
Willimantic,Connecticut

Summary

Developing and implementing solutions to challenging material, chemical, biophysical, and biochemical research problems. Experience in Carbon-based materials (CNT, Graphene, and GQDs) and other 2D nanomaterials (MoS2) exfoliation and their use in battery, supercapacitor, and energy-related applications. Experience in design, synthesis, and analysis of protein conjugates, protein-nucleic acid nanocomplexes, medicinal molecules, fluorescent molecular probes, Enzyme kinetics, and biocatalysis for pharmaceutical, biomedical applications.

Overview

8
8
years of professional experience
11
11
years of post-secondary education

Work History

Graduate Research Assistant

University Of Connecticut
Willimantic, CT
08.2018 - 08.2022
  • Project: Exfoliation of Biological 1D and 2D Nanomaterials and Fabrication of Flexible Electrodes for Energy storage and Enzyme Catalysis Applications
  • Exfoliation of 1D (Carbon nanotubes, CNT) and 2D nanomaterials (Graphene, MoS2, WS2, Zirconium Phosphate) by proteins and achieved minimum exfoliation efficiencies up to 10 mg/ml as aqueous dispersions.
  • Fabricated flexible and high conductive Graphene/CNT electrode films (36,000 S/m) for energy storage applications
  • Improved Glucose oxidase (GOx) and peroxidase (HRP) enzyme activity rate (up to 3 fold) by cascade bi-enzyme catalysis.
  • Fabricate Zn-Air batteries and bio-supercapacitor as energy storage devices by using CNT/Graphene electrode films.
  • Design and synthesis of protein-nanomaterials hydrogels and sponges for bio-catalysis and energy-efficient applications.

Project: Engineered Protein-RNA nanoparticles for effective long-distance transport, delivery, and gene silencing in plants

  • Synthesis of various cationic protein (cBSA) conjugates and protein nanocomplexes for efficient gene delivery in plants and animal nanobiotechnology applications.
  • Cationic protein/small-interfering RNA (siRNA) nanocomplex technology were most efficient at effective long-distance transport siRNA delivery and inducing gene silencing in plant stem and leaves
  • Design and synthesized Graphene quantum dots and protein fluorescent nanoprobes for biomedical and immunology studies
  • Design and analyze aggregation behavior of poly L-glutamic acid (PLG) into amyloid fibrils catalyzed and accelerated by cationic silica nanoparticle clusters modeled for supramolecular polymerization process.

Research Associate

Sanofi-Genzyme
Framingham, MA
01.2018 - 06.2018
  • Project: Bio-Pharmaceutical Quality control and Analysis of Monoclonal Antibodies and Proteins
  • Development and validation of antibodies through SDS-PAGE (Coomassie blue, silver stain), protein assays
  • Established quality control tests and validation protocols for monoclonal antibodies through RP-HPLC, SEC, HIC chromatography techniques

Research Assistant

University of Bridgeport
Bridgeport, USA
08.2015 - 01.2018
  • Project: Design and synthesis of Graphene Quantum dots and Fluorescent probes for Biomedical Applications
  • Bioconjugation of antibodies and proteins with Graphene quantum dots(GQDs)-succinimidyl esters (NHS), GQD-maleimide fluorescent probes for bioimaging applications
  • Planning, development and optimization of synthetic routes/strategies, execution and delivery of protein conjugate reactions.
  • Purification, biophysical characterization and analysis of bioconjugate proteins, organic samples, and fluorescent probes

Project Assistant

CSIR-Central Drug Research Institute, CDRI
, India
07.2013 - 06.2014
  • Department of Medicinal chemistry and Pharmaceutical Divison
  • Project: Design and synthesis 2H-Pyranone, Pyrimidone and Peptide derivatives as DPP-IV inhibitors and anti-diabetic agents
  • Synthesis of Donor-Acceptor π-conjugated aromatic scaffolds and their fluorescent properties.
  • Carried reactions peptide chemistry and macromolecules synthesis and purification of molecules through column, flash, combiflash RF chromatography techniques
  • Purification of medicinal compounds through crystallization, precipitation, lyophilization, extraction techniques

Education

Ph.D. - Material Science and Chemistry

University of Connecticut
Storrs, CT
08.2018 - 08.2022

MS - Biomedical Engineering

University of Bridgeport
Bridgeport, CT
08.2015 - 05.2017

MS - Medicinal And Pharmaceutical Chemistry

National Institute of Pharmaceutical Education & Research (NIPER)
08.2012 - 06.2014

Pharmaceutical Sciences

Andhra University
Vizag, Andhra Pradesh
07.2008 - 05.2012

Skills

GMP, GLP in Laboratory Testing & Validation

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Accomplishments

* Analytical Assays: Protein purification and analytical characterization by SEC, Analytical gel Filtration (GFC), HIC, AKTA, RP-HPLC, UPLC, Agarose & SDS-PAGE electrophoresis, Enzyme kinetics & assay development
* Biophysical Characterization: Circular dichroism (CD), UV-Vis & Fluorescence Spectroscopy, TGA, DSC, Zeta Potential, Dynamic light scattering (DLS)
* Organic synthesis: Multi-step organic reactions, Protein conjugation, Protein-nanocomplexes synthesis, Thin-layer Chromatography (TLC), Column chromatography, 1D NMR (Nuclear magnetic resonance), Mass spectrometry, UV-Visible, FT-IR spectroscopy
* Nanomaterials exfoliation and characterization: Exfoliation of 1D and 2D nanomaterials by proteins, Raman spectroscopy, Electron microscopy (TEM, SEM), X-ray diffraction, Thermal and electrical conductivity studies

* Electrochemistry techniques: Cyclic voltammetry (Pentiostat), Galvanostatic charge/discharge, Electrochemical impedance (EIS) spectroscopy
* Software's: NAMD/VMD simulation, Chem draw, Chemstation, Origin Pro, KaleidaGraph, MS office

Additional Information

  • LinkedIn: https://www.linkedin.com/in/
  • AWARDS & FELLOWSHIPS, RESEARCH WEBLINKS

Work Availability

monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Publications

1. Mansi, M., Megan, P., Kalluri, A., Kumar, C. Adsorption of metal ions on graphene sheet for applications in environmental sensing and wastewater treatment. Sensors and Actuators Reports, 2022, 4, 100077. DOI: 10.1016/j.snr.2022.100077

2. Basu, S., Bhukya. V.N., Kalluri, A., Patra, P., Sarkar, J. Self-assembly of graphene nanoparticles on biocompatible polymer through dewetting. Surfaces and Interfaces, 2021, 23, 101009. DOI: 10.1016/j.surfin.2021.101009

3. Tianjian Yang, Kyle Benson, Hailin Fu, Tianrui Xue, Ziyuan Song, Hanyi Duan, Hongwei Xia, Ankarao Kalluri, Jie He, Jianjun Cheng, Jianjun Cheng, Challa V. Kumar, and Yao Lin. Modeling and designing particle-regulated amyloid-like assembly of synthetic polypeptides in aqueous solution.

Biomacromolecules 2022, 23, 1, 196–209. DOI: 10.1021/acs.biomac.1c01230

4. Basu, S., Bhukya. V.N., Kalluri, A., Patra, P., Sarkar, J. Dewetting assisted self-assembly of graphene nanoparticles by diverse approaches. Bulletin of Materials Science, 2021, 44, 246. DOI: 10.1007/s12034-021-02535-x

5. Rawat, S., Jain, K.G., Gupta, D., Raghav, P.K., Chaudhuri, R., Shakeel, A., Arora, V., Sharma, H., Debnath, D., Kalluri, A., Agrawal, A., Jassal, M., Dinda, M., Patra, P., Mohanty, S. Graphene nanofiber composites for enhanced neuronal differentiation of human mesenchymal stem cells. Nanomedicine, 2021, 16 (22), 1963-1982. DOI: 10.2217/nnm-2021-0121

6. Jeong, S., Pinals, R.L., Dharmadhikari, B., Song, H., Kalluri, A., Debnath, D., Wu, Q., Ham, M.H., Patra, P., Landry, M.P. Graphene quantum dot oxidation governs noncovalent biopolymer adsorption. Sci Rep, 2020, 10, 7074. DOI: 10.1038/s41598-020-63769-z

7. Kalluri, A., Megan, P., Mansi, M., Kumar, C. Exfoliated and water-dispersible biocarbon nanotubes for enzymology applications. Methods in Enzymology, 2020, 630, 407-430.

DOI: 10.1016/bs.mie.2019.11.010.

8. Kalluri, A., Debnath, D., Dharmadhikari, B., Patra, P. Graphene quantum dots: Synthesis and applications. Methods in Enzymology, 2018, 609, 335-354. DOI: 10.1016/bs.mie.2018.07.002

9. Kokal, R., Melepurath, D., Kalluri, A., Singh, S., Macwan, I., Patra, P., Gilrade, J. Solar cells with PbS quantum dot sensitized TiO2–multiwalled carbon nanotube composites, sulfide-titania gel, and tin sulfide coated C-fabric. Phys. Chem. Chem. Phys. 2017, 19, 26330-26345. DOI: 10.1039/C7CP05582J

10. Kolay, A., Kokal, R., Kalluri, A., Macwan, I., Patra, P., Partha, G., Melepurath, D. New antimony selenide/nickel oxide photocathode boosts the efficiency of graphene quantum dots co-sensitized solar cell. ACS Applied Materials and Interface, 2017, 9, 34915–34926.

DOI: 10.1021/acsami.7b09754.

11. Xing, J., Singh, S., Zhao, Y., Duan, Y., Guo, H., Hu, C., Ma, A., George, R., James, Z., Xing, A., Kalluri, A., Macwan, I., Patra, P., Chen, J. Increasing vaccine production using pulsed ultrasound waves. PLoS One, 2017. DOI: 10.1371/journal.pone.0187048.

Awards and Fellowships

2020 Accelerate UConn Program

2021-2022 Graduate Research Assistant Fellowship, UConn, CT, USA

2018-2020 Graduate Teaching Assistant Fellowship, UConn, CT, USA

2016-2017 Graduate Research Assistant fellowship, University of Bridgeport, CT, USA

2017 Membership and excellence award by the Honor Society of Phi Kappa Phi.

2017 Best student service award in Department of Biomedical Engineering, University of Bridgeport, USA.

2012-2014 Research fellowship awarded by National Institute of Pharmaceutical Education & Research (NIPER), Raebareli, UP, India

Research weblinks

LinkedIn: https://www.linkedin.com/in/ankarao-kalluri-35679411b/

Google scholar: https://scholar.google.com/citations?user=PNNNWIYAAAAJ&hl=en

Research Gate: https://www.researchgate.net/profile/Ankarao_Kalluri

Timeline

Graduate Research Assistant

University Of Connecticut
08.2018 - 08.2022

Ph.D. - Material Science and Chemistry

University of Connecticut
08.2018 - 08.2022

Research Associate

Sanofi-Genzyme
01.2018 - 06.2018

MS - Biomedical Engineering

University of Bridgeport
08.2015 - 05.2017

Research Assistant

University of Bridgeport
08.2015 - 01.2018

Project Assistant

CSIR-Central Drug Research Institute, CDRI
07.2013 - 06.2014

MS - Medicinal And Pharmaceutical Chemistry

National Institute of Pharmaceutical Education & Research (NIPER)
08.2012 - 06.2014

Pharmaceutical Sciences

Andhra University
07.2008 - 05.2012
Ankarao KALLURIMaterial & Protein Engineer | Analytical & Medicinal Chemist