Arun Singh

Skilled multitasker with superior work ethic and good teamwork, problem-solving and organizational skills. Willing to take on any task to help team. Reliable and dedicated team player with hardworking and resourceful approach. Motivated Student currently pursuing Master of Science degree in Chemical Engineering. Eager to expand horizons and make positive impact by taking on hands-on, dynamic challenges in academic life.

I am working independently on a project to determine the impact of the chaperones such as calnexin and calreticulin knockouts on HEK cells. As in normal conditions, chaperons are responsible for the proteins to acquire their native shapes by providing them with a separate environment and/or increasing their retention time in the endoplasmic reticulum. As glycosylation depends on the shape, structure, and exposed sites of the proteins, nonnative structure results in different glycosylation patterns that can be captured in the glycomics profile of the cells. Cell Machinery also has a quality control mechanism that checks predominantly, the shape of the proteins and does not allow further processing of the proteins if their shapes deviate significantly from their native shape that can be captured in proteomics experiments. Comparing the results from the lectin study, proteomics, and glycomics experiments will provide some insight into the role of the chaperons. As of now, we are expecting that heavily glycosylated, multi-domain proteins depend more on chaperons to get their native shapes and thus their abundance in the knockouts should be reduced compared to the wild-type HEK cells. I am expecting to get a first-author paper published by the end of my master's course (May -2023).