Summary
Overview
Work History
Education
Skills
Papers Presented
Teaching Experiences
Advising And Mentorship Experiences
Fellowships And Awards
Publications
Additional Information
Languages
Work Availability
Quote
Timeline
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ASENO SAKHRIE

ASENO SAKHRIE

PhD candidate
Storrs,CT

Summary

To seek and maintain full-time position that offers professional challenges utilizing interpersonal skills, excellent time management and problem-solving skills.

Overview

11
11
years of professional experience

Work History

Intern

AVSbio Storrs, CT
07.2023 - 10.2023
  • Acquired hands-on experience in quality control, record-keeping, and the preparation of reports for their product line, adhering to GMP guidelines to maintain product consistency, safety, and efficacy.
  • Played a role in the processing of USDA licensed FFM product lines (BDA, Bursas).
  • Contributed to the training of some employees in various molecular techniques.
  • Supported staff members in their daily tasks, reducing workload burden and allowing for increased focus on higher-priority assignments.

Junior Research Fellow

ICAR-National Institute of High Security Animal Diseases
07.2016 - 09.2017
  • Worked under the project titled “Development of Multiplex diagnostic assay for all prioritized BSL-III/IV pathogens for North-Eastern Region viz Nipah Virus, Reston Ebola Virus, Japanese Encephalitis Virus, Hantaan Virus, Bunyaviruses (CCHFV, RVF, and NSD), etc.
  • Worked in a BSL-III/IV facility with strict safety protocols and handled BSL-III/IV pathogens.
  • Learned collection of samples from bat, guinea pigs and mosquitoes in lab condition as well as in the wild.
  • Acquired skills on cell culture techniques, multiplex PCR, qPCR, bacterial cloning and construct designs
  • Designed and carried out complex laboratory experiments for standardization and to assess effectiveness of in-house strip tests.
  • Managed laboratory resources efficiently, ensuring timely completion of experiments and projects.
  • Maintained accurate records of experiments and results, ensuring reliability and replicability in future studies.
  • Worked as member of integrated project team in highly collaborative work environment.

Block Technology Manager (BTM)

Agriculture Technology Management Agency, Department of Agriculture
05.2015 - 05.2016
  • Managed implementation of new technological improvements resulting in increased efficiency to farmers
  • Managed technology projects from conception to completion, ensuring timely delivery and stakeholder satisfaction.
  • Monitored employee tasks to gauge business functions and inefficiencies.
  • Developed suggestions for technical process improvements to optimize resources for farmers.
  • Trained farmers and dependents on agriculture food product processing to generate income.
  • Formation and strengthening of farmer's interest group.

Junior Research Fellow

National Research Centre on Mithun (ICAR)
06.2013 - 08.2014
  • Worked on DBT project “Epidemiology and genetic characterization of some important helminth parasites of mithun (Bos frontalis) from north eastern hilly region of India.
  • Skilled at working independently and collaboratively in a team environment.
  • Managed time efficiently in order to complete all tasks within deadlines.
  • Demonstrated respect, friendliness and willingness to help wherever needed.
  • Proved successful working within tight deadlines and a fast-paced environment.
  • Cultivated interpersonal skills by building positive relationships with others.

Junior Research Fellow

Department of Veterinary Microbiology, College of Veterinary Sciences and AH, CAU
09.2012 - 05.2013
  • Worked on DBT, Govt of India Sponsored Twinning project on “Serosurveillence and Molecular Epidemiology of Peste des petits ruminants(PPR), Sheep Pox and Goat pox in Sheep and goats of Tripura and Mizoram states.
  • Adaptable and proficient in learning new concepts quickly and efficiently.
  • Paid attention to detail while completing assignments.
  • Worked flexible hours across night, weekend and holiday shifts.
  • Used critical thinking to break down problems, evaluate solutions and make decisions.
  • Developed and maintained courteous and effective working relationships.
  • Organized and detail-oriented with a strong work ethic.

Education

Ph.D. - Virology (Avian Vaccine)

University of Connecticut
Storrs, CT
05.2024

Master’s of Veterinary Sciences - Veterinary Microbiology

Guru Angad Dev Veterinary And Animal Sciences University
Ludhiana, India
08.2012

Bachelor’s of Veterinary Sciences and Animal Husbandry -

College of Veterinary Sciences And Animal Husbandry, Central Agricultural University
Mizoram, India
08.2005

Skills

  • DNA isolation
  • RNA isolation
  • Plasmid and genomic DNA isolation
  • Gene cloning from bacteria
  • PCR
  • qPCR
  • UV-VIS
  • SDS PAGE
  • Western Blotting
  • Virus Isolation
  • Cell Culture
  • Egg inoculation
  • Fluorescent microscopy
  • Flow cytometry
  • Mouse and rat handling
  • Bat handling
  • Chicken handling
  • MS word
  • MS excel
  • R
  • KaleidaGraph
  • GraphPad Prism
  • Training and Mentoring
  • Equipment Maintenance
  • Research Planning
  • Experimental design
  • Grant writing
  • Quality Control
  • Project Management
  • Team Collaboration

Papers Presented

  • Oral presentation on “Comparison of Canine Parvovirus Field Isolates and Vaccine Strain by Sequence Analysis” at Canine Parvovirus Symposium entitled “Current Status of Canine Parvovirus Infection in India” from January 23rd-24th, 2013 organised by Rajiv Gandhi College of Veterinary Sciences, Puducherry.
  • Poster presentation on “Immature Amphistomiasis in Mithun - a case report” at National Symposium on “Advances and Applications of Diagnostic Pathology for Disease Management in Livestock, Poultry, Pet , fish Labortory Animal and Wildlife” from 21st to 23rd Nov 2013 organized by Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar-751003, Odisha.
  • Poster presentation on “Seasonal prevalence of Gastrointestinal helminth parasites in Mithun (Bos frontalis) in Porba” at National Seminar on "Emerging Challenges and Prospective Strategies for Hill Agriculture in 2050" on January 20, 2014 hosted by ICAR Center for NEH region, Dimapur-797106, Nagaland.
  • Poster presentation titled ‘Use of a novel FluoDot nanoparticle as a delivery vehicle for infectious bronchitis virus antigen’ at AVMA Convention 2021, July 30 - August 3 in Minneapolis, MN.
  • Poster presentation titled 'Study of expression of EGFP mRNA using Cationic generic protein-polyamine nanoparticle' at TechConnect World Innovation Conference 2022, June 13-15 in National Harbor, MD.
  • Abstract accepted for oral presentation titled “mRNA vaccine for IBV with cationic BSA-polyamine nanoparticle as delivery vehicle” at Conference of Research Workers in Animal Disease (CRWAD) 2022, January 20-23 in Chicago, IL.

Teaching Experiences

  • PATH 1000, Biomedical Issues in Pathobiology, Pathobiology and Veterinary Science, Fall 2023
  • PATH 2100, Anatomy and Physiology of Animals, Pathobiology and Veterinary Science, Fall 2023
  • PATH 1000, Biomedical Issues in Pathobiology, Pathobiology and Veterinary Science, Fall 2022
  • PATH 2100, Anatomy and Physiology of Animals, Pathobiology and Veterinary Science, Fall 2022
  • PVS 2301, Health and Disease Management of Animals, Pathobiology and Veterinary Science, Spring 2022
  • PVS 1000, Biomedical Issues in Pathobiology, Pathobiology and Veterinary Science, Fall 2021
  • PVS 2100, Anatomy and Physiology of Animals, Pathobiology and Veterinary Science, Fall 2021
  • PNB 2265, Human Physiology and Anatomy, Physiology and Neurobiology, Spring 2021

Advising And Mentorship Experiences

  • Provided guidance, mentorship and training to undergraduate students on laboratory techniques.
  • Wrote reference letters for multiple undergraduates to support their applications for scholarships, internships, and graduate programs.

Fellowships And Awards

  • Pre-doctoral Fellowship Issued by Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 2023
  • CRWAD student travel award issued by Conference of Research Workers in Animal Disease (CRWAD) 2022
  • Pre-doctoral Fellowship Issued by Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 2022
  • Conference Participation Award issued by The Graduate School, University of Connecticut, Storrs, CT 2021
  • Pre-doctoral Fellowship Issued by Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 2021
  • National Overseas Scholarship for Scheduled Tribe (2015-16) from Ministry of Tribal affairs, India
  • Recipient of Indian Council of Agricultural Research -Junior Research Fellowship, 2010

Publications

  • A Sakhrie , J Ding , A Kalluri , Z Helal , C V. Kumar and M I. Khan. Fluodot Nanoparticle - A Promising Novel Delivery System For Veterinary Vaccine. International Journal of Nanoparticle Research. IJNR, 2020; 3:14.
  • Raut AA, Aasdev A, Mishra A, Dutta B, Bharali A, Konwar N, Dubey CK, Chingtham S, Pawar SD, Raghuvanshi R, Sakhrie A, Sen A, Patil SS, Singh VP, Barman NN. Detection of coinfection of a divergent subgroup of genotype I Japanese encephalitis virus in multiple classical swine fever virus outbreaks in pigs of Assam, India. Transbound Emerg Dis. 2020 Oct 30. doi: 10.1111/tbed.13903. Epub ahead of print. PMID: 33124192.
  • J. K. Chamuah, A. Sakhrie, P. Perumal. Khate, K. Vupru & C. Rajkhowa (2013) Seroprevalence of Toxoplasma gondii in mithun (Bos frontalis) from north eastern hilly region of India. Journal of Parasitic Disease DOI 10.1007/s12639-013-0396-0
  • A. Sakhrie, G. Kaur, M. Chandra, D. Deka and P. N. Dwivedi (2014). Comparison of field and vaccine isolates of Canine Parvovirus on the basis of partially sequenced VP2 region. Veterinary Practitioner 15(1): 15-19.
  • J.K. Chamuah, S. Jacob, A. Sakhrie and D. Borkotoky. Serological prevalence of Fasciola gigantic in Mithun (Bos frontalis). International Journal of Livestock Research. DOI 10.5455/ijlr.20140730091452.
  • J.K Chamuah, S.R Pegu, O.K Raina, S.J. Siju, A.Sakhrie, Anil Deka and C. Rajkhowa Pimply gut condition in Mithun (Bos frontalis) calves. Journal of parasitic diseases. DOI:10.1007/s12639-014-0487-6
  • D. Borkotoky, J.K. Chamuah, A. Sakhrie P. R. Dutta, N. Ebibeni and K. Lovingson. Occurance of Gastrointestinal helminth parasites of Indigenous local pigs in Phek District of Nagaland. International Journal of Livestock Research DOI: 10.5455/ijlr.20140920085619.
  • J.K. Chamuah, A. Sakhrie, S. Lama, S. Chandra, G. M. Chigude, R. K. Bauri and S. J. Siju. Molecular characterization of Setaria digitata from Mithun (Bos frontalis). Acta Parasitologica. DOI: 10.1515/ap-2015-0054.
  • J.K Chamuah, O. K Raina, H. Lalrinkima, S. S Jacob, M. Sankar, A. Sakhrie, S. Lama, and P. S Banerjee. Molecular characterization of veterinary important trematode and cestode species in the mithun Bos frontalis from north-east India. Journal of Helminthology. DOI: 10.1017/S0022149X15000772.
  • J.K Chamuah, P. R. Dutta, Ved Prakash, O. K. Raina, A. Sakhrie, D. Borkotoky, P. Perumal, R. Neog and C. Rajkhowa. Comparative efficacy of some plant extracts on Rhipicephalus (Boophilus) microplus infestation on Mithun (Bos frontalis) in the north-east. Journal of Veterinary Parasitology. 27 (1) 2013: 5-7.
  • N. Ebibeni, J.K Chamuah, O. K. Raina, A. Sakhrie, P. Perumal, D. Borkotoky and N. Lily. Prevalence of gastrointestinal parasites of pigs in Dimapur district (Nagaland). Journal of Veterinary Parasitology (Short Communication). 27(1) 2013: 57-58.
  • Raut AA, Aasdev A, Mishra A, Dutta B, Bharali A, Konwar N, Dubey CK, Chingtham S, Pawar SD, Raghuvanshi R, Sakhrie A, Sen A, Patil SS, Singh VP, Barman NN. Detection of coinfection of a divergent subgroup of genotype I Japanese encephalitis virus in multiple classical swine fever virus outbreaks in pigs of Assam, India. Transbound Emerg Dis. 2020 Oct 30. doi: 10.1111/tbed.13903. Epub ahead of print. PMID: 33124192.
  • A. Sakhrie, J. Ding, A Kalluri, Z Helal, C. V. Kumar and M. I. Khan. Fluodot Nanoparticle – A Promising Novel Delivery System For Veterinary Vaccine. International Journal of Nanoparticle Research, 2020; 3:14. DOI: 10.28933/ijonr-2020-08-1205.

Additional Information

Professional Memberships:

  • Member of Institute of Food Technologist Student Association (IFTSA)
  • Member of American Association of Avian Pathologist (AAAP)

Volunteer Work:

  • Volunteered at Connecticut Foodshare by helping bagging food items to support individuals and families.
  • Volunteered at Nursing home Vanderman Place, CT providing musical performances and companionship to residents through singing.

Personal accomplishments:

  • Participated in Mudgirl obstacle race, Hartford, CT in 2023, dedicated to women to improve communities well-being and to foster a collective spirit of solidarity, while preserving the environment
  • Completed 25th Annual South Park Road Race and Fitness Walk on the 5k USTAF certified course organized by University of Connecticut Health in 2022 showcasing dedication, discipline, and commitment to personal challenges.
  • Vice president and treasurer for International Christian Fellowship, University of Connecticut, Storrs, CT from Fall 2019 to Fall 2021.

Languages

English
Full Professional
Hindi
Limited Working

Work Availability

monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Quote

Far and away the best prize that life offers is the chance to work hard at work worth doing.
Theodore Roosevelt

Timeline

Intern

AVSbio Storrs, CT
07.2023 - 10.2023

Junior Research Fellow

ICAR-National Institute of High Security Animal Diseases
07.2016 - 09.2017

Block Technology Manager (BTM)

Agriculture Technology Management Agency, Department of Agriculture
05.2015 - 05.2016

Junior Research Fellow

National Research Centre on Mithun (ICAR)
06.2013 - 08.2014

Junior Research Fellow

Department of Veterinary Microbiology, College of Veterinary Sciences and AH, CAU
09.2012 - 05.2013

Ph.D. - Virology (Avian Vaccine)

University of Connecticut

Master’s of Veterinary Sciences - Veterinary Microbiology

Guru Angad Dev Veterinary And Animal Sciences University

Bachelor’s of Veterinary Sciences and Animal Husbandry -

College of Veterinary Sciences And Animal Husbandry, Central Agricultural University
ASENO SAKHRIEPhD candidate