Ashley Alamillo
San Diego,CA
Summary
Dedicated and detail-oriented Associate Scientist with extensive experience in primary immune cell assays, flow cytometry, cell culture, and molecular biology techniques. Proven track record in supporting drug discovery teams with robust data packages. Skilled in designing, executing, and troubleshooting complex experiments. Committed to staying current with novel scientific developments and implementing them in the laboratory.
Overview
9
9
years of professional experience
Work History
Associate Scientist
Calidi Biotherapeutics
10.2018 - Current
- Culture and cryopreserve human stem cells, murine and human tumor cell lines.
- Isolate, cultivate, and cryopreserve human primary immune cells such as PBMCs, NK cells and γδ T cells.
- Lead immunogenicity program on pre-clinical product, SuperNova, to generate degranulation data to support Pre-IND/Clinical trial submission.
- Collaborate in optimizing SuperNova dose formulation for the in vivo biodistribution and toxicity studies.
- Conduct immune cell mediated cytotoxicity assays using xCELLigence RTCA.
- Immunophenotype cell surface as well as intracellular markers on various samples via FACS analysis (multi-color parameter assays).
- Design and perform multiplex cytokine assays using the MSD platform to evaluate cytokine secretion in PBMCs and SuperNova co-cultures.
- Develop and perform assay to characterize expression of virus surface markers in purified viral samples via flow virometry.
- Assist with generating and selecting lead engineered enveloped vaccinia virus for RTNova program by testing various transfer vectors via transfection.
- Analyze data using GraphPad and FlowJo.
Research Scientist
AnaBios Corporation
11.2015 - 10.2018
- Processed incoming human organs such as hearts, DRGs (dorsal root ganglion), livers, brains, spleens.
- Performed DRG dissection, dissociation and cultivated neuronal cells for electrophysiology assays.
- Isolated and cultured adult human hepatocytes.
- Conducted action potential and contraction experiments on human cardiac tissue.
- Dissociated human cardiomyocytes and performed functionality assays to test drug safety and therapeutic drug discovery.
- Developed new methodology for various projects.
- Trained junior researchers in laboratory techniques and scientific principles.
- Analyzed data and drafted reports.
- Established standard operating procedures SOPs for laboratory safety and quality control measures.
Education
Bachelor of Arts (B.A.) - Biology -
California State University, Northridge
05.2015
Skills
- Experimental design
- Cell-based assays
- Cell culture
- Primary immune cell isolation
- Immunoassays
- DNA extraction
- Gel electrophoresis
- Data analysis
- Flow cytometry
- Small-scale virus production
Languages
Spanish
Native or Bilingual
Timeline
Associate Scientist
Calidi Biotherapeutics
10.2018 - Current
Research Scientist
AnaBios Corporation
11.2015 - 10.2018
Bachelor of Arts (B.A.) - Biology -
California State University, Northridge
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