Ashlyn Maria
Buckhorn BBQ & Grill
Vacaville,California
5
years of professional experience
Experienced with academic research methodologies, data analysis, and literature review. Utilizes critical thinking and problem-solving skills to advance research projects effectively. Skilled in scientific writing and collaborative teamwork, ensuring reliable, high-impact results.
Work History
Undergraduate Researcher - Protein and Enzyme Biotechnology
3 Months- Utilized Foldit for in-silico mutant protein design to generate hypotheses for site-directed mutagenesis.
- Executed Kunkel mutagenesis protocols, including oligo phosphorylation and transformation into DH5α cells.
- Conducted large-scale bacterial expression in BL21(DE3) strains and purified proteins using Immobilized Metal Affinity Chromatography (IMAC).
- Quantified protein concentration via A280 and Bradford assays and verified purity through SDS-PAGE and Western Blot development.
- Characterized functional parameters through colorimetric enzyme kinetics and thermostability assays.
- Submitted validated enzyme data to the D2D national protein biotechnology database.
Undergraduate Researcher - Educational Research (Biochemistry CURE)
3 Months- Collaborated on transitioning science pedagogy from traditional demonstrations to active research practitioner models.
- Beta-tested and refined a "Design-Build-Test-Learn" engineering progression for undergraduate biochemistry curriculum.
- Participated in data analysis regarding gains in scientific identity and self-efficacy among STEM students.
- Evaluated the efficacy of industrially relevant workflows in a classroom setting to ensure reproducible research results.
- Research Data Contributions
- Contributed experimentally validated enzyme data to the Design to Data (D2D) national protein biotechnology database (UC Davis)
Restaurant Server and Bartender
3 Years 6 Months- Awarded Employee of the Year for outstanding performance and reliability.
- Delivered high-quality customer service in a fast-paced environment.
- Maintained strict food safety and sanitation standards.
- Resolved customer issues quickly through strong problem-solving and time management
Restaurant Server and Bartender
3 Years 6 Months- Received Employee of the Year recognition for outstanding performance and reliability.
- Delivered high-quality customer service in a fast-paced environment.
- Maintained strict food safety and sanitation standards.
- Resolved customer issues quickly through strong problem-solving and time management
Restaurant Shift Leader
2 Years 2 Months- Supervised and trained staff, fostering strong teamwork and accountability.
- Ensured high-quality service and operational efficiency in a fast-paced setting.
- Maintained strict cleanliness and food safety standards.
Education
Bachelor of Science - Biochemistry and Molecular Biology, Minor in Global Disease Biology
Skills
Laboratory: Site-directed mutagenesis (Kunkel method)
Protein purification (IMAC
Ni-NTA microcolumn
GFP-tagged proteins)
SDS-PAGE
Western blotting
PCR
Gel electrophoresis
Spectrophotometry
Bradford and A280 assays
Enzyme kinetic assays
Microscopy
Buffer Preparation
Pipetting.
Computer: Foldit (protein design)
Benchling
Microsoft Excel
R Programming Language
RELEVANT COURSEWORK
Advanced Molecular Biology, Macromolecular Structure and Function, Bioenergetics and Metabolism, Human Immunology, Cancer Biology
Accomplishments
• Contributed experimentally validated enzyme data to the Design to Data (D2D) national protein biotechnology database (UC Davis)
Timeline
Undergraduate Researcher - Protein and Enzyme Biotechnology
Design2Data (D2D) Program, UC Davis
09.2025 - 12.2025Read More
Undergraduate Researcher - Educational Research (Biochemistry CURE)
Design2Data (D2D) Program, UC Davis
09.2025 - 12.2025Read More
Restaurant Server and Bartender
Buckhorn BBQ & Grill
04.2023 - CurrentRead More
Restaurant Server and Bartender
Buckhorn BBQ & Grill
04.2023 - CurrentRead More
Restaurant Shift Leader
Baskin Robbins
07.2021 - 09.2023Read More
University of California, Davis
Bachelor of Science from Biochemistry and Molecular Biology, Minor in Global Disease Biology
Read More