Ashna Ambrin Hoque

• Performed site-directed mutagenesis to generate Beta lactamase mutants.
• Conducted bacterial spot plating assay to study antibotic senstivity.
• Completed 8 molecular cloning projects to produce E.coli with Canavanyl tRNAArg deacylase (CtdA) genes from 3 different bacterial species.
• Performed E.coli growth assays to analyze the function of the CtdA genes.
• Participated actively in lab meetings every week with team members to discuss updates on projects and challenges faced.
• Organized research materials and maintained lab notebooks.
• Designed and performed experiments while maintaining biosafety standard protocols
• Presented research data at lab meetings

• Investigated the role of the protein EGG-3 in egg activation and its impact in later development in C. elegans.
• Performed fluorescent microscopy to study the eggshell formation in mutant C. elegans worms.
• Formulated a suppressor screen to identify genetic interactors of EGG-3 and isolated six suppressors.
• Designed and performed experiments related to assigned projects
• Performed detailed data analysis and presented data at lab meetings.
• Presented research in two seminars and three poster presentations.

• Taught 3 introductory biology lab courses to over 40 students for 4 semesters.
• Planned and delivered 9-10 lectures and course materials per term to communicate course content and provide students with subject matter knowledge.
• Mentored students through office hours and one-on-one communication.
• Documented attendance to maintain full class and student records.
• Proctored tests and and graded assignments, lab reports according to university standards.
• Worked with course instructor to optimize learning plans based on student test grades and evaluations.

• Assisted in Teaching 3 college-level Biology and Chemistry courses.
• Utilized online video chat platforms to instruct students remotely.
• Created PowerPoint Presentations to give lectures to students.
• Graded assignments and quizzes according to university standards.
• Conducted review sessions to help students prepared for tests.
• Worked with students to help them solve assignments and identify weaknesses.

Received training on -

• Antibiotic sensitivity test and Extreme Spectrum Beta Lactamase (ESBL) test on over 20 patient samples.
• Biochemical tests for microbial identification (Kliger's Iron Agar, Motility Indole Urease, Citrate Utilization, Oxidase, Catalase, CAMP test) on over 20 patient samples.
• DNA and RNA isolation from whole blood samples.
• Real-time PCR particularly focused on detecting HLA-B27, BCR-ABL.