Ashutosh Pandey
Newark,USA
Summary
Dynamic Research Associate II at Rutgers New Jersey Medical School with expertise in protein purification and chromatography. Proven track record in optimizing yield and scalability, while mentoring junior scientists. Skilled in analytical characterization methods and drafting SOPs, complemented by strong communication abilities to present complex data effectively.
Overview
15
15
years of professional experience
Work History
Research Associate II
Rutgers New Jersey Medical School
Newark, USA
08.2017 - Current
- Lead and drive protein purification process development activities for both existing and new products, focusing on optimizing yield, purity, and scalability.
- Design and implement robust purification strategies utilizing chromatographic techniques, including affinity, ion-exchange, size exclusion, depth filtration, and tangential flow filtration (TFF).
- Apply in-depth knowledge of downstream processing to facilitate seamless transitions from lab-scale to production-scale operations.
- Conducted protein characterization using analytical techniques such as FPLC-ÄKTA, HPLC, SDS-PAGE, UV-Vis, Western blot, LC-MS, and SEC-MALS.
- Supervise and conduct experimental work related to new product development, ensuring high-quality data generation and reproducibility.
- Oversee and mentor Associate Scientists, providing guidance on experimental design, data analysis, and troubleshooting.
- Perform protein manufacturing following purification batch records and ensure adherence to regulatory and quality control standards.
- Draft standard operating procedures (SOPs), batch record templates, and associated documentation for protein purification processes.
- Conduct thorough protein characterization and analysis, assisting in troubleshooting equipment and experimental challenges.
- Collaborate closely with cross-functional teams, including quality control and quality assurance, to meet project milestones and regulatory requirements.
- Contribute to the development of long-range research and development plans, aligning with laboratory objectives.
- Maintain and advance expertise in areas relevant to company products and strategic goals.
- Present research findings and data analysis to internal departments and project teams.
Postdoctoral Scientist
Rutgers New Jersey Medical School
Newark, USA
11.2012 - 07.2017
- Designed and executed protein purification workflows, ensuring high purity and activity.
- Conducted protein characterization using analytical techniques such as HPLC, SDS-PAGE, UV-Vis, Western blot, LC/MS and SEC-MALS.
- Developed purification strategies for proteins expressed in inclusion bodies, optimizing protein renaturation/refolding conditions.
- Applied Design of Experiments (DoE) methodologies to optimize purification parameters and enhance process robustness.
- Assisted in protein formulation development, designing buffer formulations to improve protein solubility, stability, and shelf-life.
- Developed lyophilization protocols with cryoprotectants and lyoprotectants for long-term protein storage.
- Guided 3-4 junior students and undergraduates in laboratory techniques and assay design.
Research Scientist
Cheminova India Ltd
Ankleshwar, India
08.2010 - 09.2012
- Participated in a team focused on conducting R&D experiments, including the synthesis and characterization of small molecules, qualitative and quantitative analyses, daily experimentation, and documenting results according to GMP standards.
- Performed experiments using GC and HPLC, and employed various wet chemistry techniques including organic synthesis, crystallization, purification, distillation, TLC, and other chromatographic methods.
Education
Ph.D. - Chemistry
V.B.S Purvanchal University/Banaras Hindu University
India01.2011
Master's degree - Organic chemistry
V.B.S Purvanchal University
Uttar Pradesh, India07.2004
Bachelor's degree - Biology and Chemistry
V.B.S Purvanchal University
Uttar Pradesh, India07.2002
Skills
- Protein purification techniques
- Chromatography
- Depth filtration
- TFF
- Purification of proteins from inclusion bodies
- Optimization of renaturation/refolding conditions
- Recombinant protein construct design
- Protein expression
- Analytical protein characterization methods
- FPLC-AKTA
- HPLC
- SDS-PAGE
- UV-visible spectrophotometry
- Western blot
- LC/MS
- SEC-MALS
- Experimental design
- DoE methodologies
- Protein formulation development
- Buffer design
- Lyophilization protocols
- Communication skills
- Technical writing skills
- Drafting SOPs
- Batch records
- Scientific documentation
Websites
References
Available upon request.
Publications
- Salutary effects of overexpression of Rsm22, an assembly factor for the mitochondrial ribosome, on frataxin/Yfh1 depletion phenotypes in Saccharomyces cerevisiae, Biomolecules, 2025, 15, 785
- Mitochondrial glutaredoxin Grx5 functions as a central hub for cellular iron-sulfur cluster assembly, Journal of Biological Chemistry, 2025, 301, 108391
- Mitochondrial acyl carrier protein, Acp1, required for iron-sulfur cluster assembly in mitochondria and cytoplasm in Saccharomyces cerevisiae, Mitochondrion, 2024, 79, 101955
- Mitochondria function in cytoplasmic FeS protein biogenesis, BBA Molecular Cell Research, 2024, 1871, 119733
- Essential mitochondrial role in iron-sulfur cluster assembly of the cytoplasmic isopropylmalate isomerase Leu1 in Saccharomyces cerevisiae, Mitochondrion, 2023, 69, 104-115
- Child Neurology: Progressive Cerebellar Atrophy and Retinal Dystrophy Clues to an Ultrarare ACO2-Related Neurometabolic Diagnosis, Neurology, 2023, 101, e1567-1571
- Characteristics of the Isu1 C-terminus in Relation to 2Fe-2S Cluster Assembly and ISCU Myopathy, Journal of Biological Inorganic Chemistry, 2022, 27, 759-773
- Inhibition of mitochondrial LonP1 protease by allosteric blockade of ATP binding and hydrolysis via CDDO and its derivatives, Journal of Biological Chemistry, 2022, 298, 101719
- Roles of host mitochondria in the development of COVID-19 pathology: Could mitochondria be a potential therapeutic target?, Molecular Biomedicine, 2021, 2, 1-19
- Mitochondria export iron-sulfur and sulfur intermediates to the cytoplasm for iron-sulfur cluster assembly and tRNA thiolation in yeast, Journal of Biological Chemistry, 2019, 294, 9489-9502
- Splitting the functions of Rim2, a mitochondrial iron/pyrimidine carrier, Mitochondrion, 2019, 47, 256-265
- Protein quality control at the interface of endoplasmic reticulum and mitochondria by LonP1, British Journal of Pharmacology, 2019, 176, 505-507
- Bi-allelic mutations of LONP1 encoding the mitochondrial LonP1 protease cause pyruvate dehydrogenase deficiency and profound neurodegeneration with progressive cerebellar atrophy, Human Molecular Genetics, 2019, 28, 290-306
- Mitochondria export sulfur species required for cytosolic tRNA thiolation, Cell Chemical Biology, 2018, 25, 738-748
- Acetylation and phosphorylation of human TFAM-DNA interaction via contrasting mechanisms, Nucleic Acids Research, 2018, 46, 3633-3642
- The β1'-β2' motif of the RNase H domain of HIV-1 RT is responsible for conferring open conformation to p66 subunit by displacing the connection domain from the polymerase cleft, Biochemistry, 2017, 56, 3434-3442
- Modulation of HCV replication and translation by ErbB3 binding protein1 isoforms, Virology, 2017, 500, 35-49
- Staufen1 promotes HCV replication by inhibiting protein kinase R and transporting viral RNA to the site of translation and replication in the cells, Nucleic Acid Research, 2016, 44, 5271-5287
- FUSE Binding Protein1 Facilitates Persistent Hepatitis C Virus Replication in Hepatoma Cells by Regulating Tumor Suppressor p53, Journal of Virology, 2015, 89, 7905-7921
- Fuse binding protein antagonizes the transcription activity of tumor suppressor protein p53, BMC Cancer, 2014, 14, 925
Timeline
Research Associate II
Rutgers New Jersey Medical School
08.2017 - Current
Postdoctoral Scientist
Rutgers New Jersey Medical School
11.2012 - 07.2017
Research Scientist
Cheminova India Ltd
08.2010 - 09.2012
Ph.D. - Chemistry
V.B.S Purvanchal University/Banaras Hindu University
Master's degree - Organic chemistry
V.B.S Purvanchal University
Bachelor's degree - Biology and Chemistry
V.B.S Purvanchal University
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