Audrey Moran
Redmond,WA
Summary
Experienced research associate with 1 year of expertise preparing plasmids and manipulating mammalian cells. Skilled in multidisciplinary collaboration, data collection and analysis, experimental procedure design, and utilizing laboratory techniques. Committed to producing meaningful and reproducible research.
Overview
1
1
year of professional experience
Work History
Undergraduate Research Assistant
University of Washington: Sarah Pierce, PhD
Seattle, Washington
11.2023 - 05.2024
- Ran PCRs and purified the products
- Created plasmid vectors containing various known SNVs via Gibson Assemblies
- Transfected Top10 competent bacteria with vectors
- Performed midi and mini preps on DNA and quantified the resulting DNA
- Performed diagnostic tests using appropriate restriction enzymes
- Transfected MCF7 breast tissue cells with vectors
- Performed Luciferase Reporter Assays to quantify the expression of the ESR1 promoter in breast tissue cells
- Planned, modified and executed research techniques, procedures and tests
- Completed BioSafety Level II (BSL2) training
- Supported faculty researchers, assisting with experiment design and implementation
Undergraduate Research Assistant
University of Washington: Jody Deming, PhD
Seattle, WA
06.2023 - 08.2023
- Extracted and purified DNA for sequencing
- Inoculated medias and fixed samples with formaldehyde
- Performed microscopy and bacteria enumeration of over 10,000 individual bacteria using the standard plate method
- Maintained detailed records of data, experiments, and techniques, and complied data into reports for supervisor to review
- Taught peers experimental methodology
- Refined experimental methods and procedure
Education
Bachelor of Science - Molecular, Cellular, And Developmental Biology
University of Washington
Seattle, WA 05.2001 -
Skills
Molecular Biology: Electrophoresis, Cell Assays, DNA manipulation, Mutation testing and screening, Molecular cloning, DNA and RNA extraction (lysis, purification, quality control), Library preparation, PCR, Use of restriction enzymes, Western blot
Cell Culturing and Bacteriology: Maintain and purifying bacteria, phage stocks, and mammalian cell cultures, Cell and tissue transfer, Culture transfer, Inoculation, Media preparation, Plasmid amplification
General Laboratory Techniques: Light microscopy (widefield), Microplate luminometer, Centrifuges (standard and high speed), Incubators, Electron microscopy (fixation and staining), Up to and including Biosafety Level II handling
Dry Lab: Data processing and analysis , Detailed record keeping, Statistical analysis, Excel, Development of procedural techniques
Timeline
Undergraduate Research Assistant
University of Washington: Sarah Pierce, PhD
11.2023 - 05.2024
Undergraduate Research Assistant
University of Washington: Jody Deming, PhD
06.2023 - 08.2023
Bachelor of Science - Molecular, Cellular, And Developmental Biology
University of Washington
05.2001 -
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