Brad Green, PhD
Senior Scientist
Gilbert,AZ
Summary
Experienced senior-level scientist with expertise in drug discovery and development. Involved in successfully developing numerous peptide-based drugs as first-in-class therapeutics for pain, epilepsy, cancer, and fibrosis. Proficient in designing, synthesizing, and characterizing macrocyclic peptide drugs using analytical techniques such as RP-HPLC, LCMS, CD spectroscopy, NMR, and more. Continuously seeking opportunities to learn new techniques and expand skill set. Also experienced in molecular biology techniques, in vitro pharmacology, and structural biology. Trained and mentored junior scientists throughout my scientific career, finding joy in sharing knowledge and expertise with those starting their scientific journey.
Overview
23
23
years of professional experience
Work History
Senior Scientist
FAKnostics, LLC
01.2022 - Current
- Conduct structure-activity relationship studies to guide the design and synthesis of stapled peptide inhibitors of focal adhesion kinase (FAK).
- Provide scientific direction for new peptide drug projects.
- Optimize peptide synthesis and purification of lead drug candidates.
- Interface with external CROs and vendors for CMC-related activities in preparation of IND submission.
- Train and supervise junior scientists working for FAKnostics and the University of Arizona Dept of Internal Medicine.
- Provide regular updates and summaries of research activities to lab group, collaborators and potential investors.
Research Fellow
Applied Biosensors, LLC
01.2021 - 01.2022
- Designed and developed peptide and oligonucleotide-functionalized hydrogels for biosensing of protein targets.
- Performed oxidative folding and purification of peptide aptamers for incorporation into IgG responsive hydrogels.
- Constructed photometric oligonucleotide aptamer-based biosensors that recognize and respond to protein targets.
- Facilitated strong communication among team members through regular meetings, progress reports, and open channels of dialogue.
- Contributed to successful grant applications by conducting preliminary studies that supported project feasibility and relevance.
- Conducted comprehensive literature reviews to identify knowledge gaps and inform future studies.
Research Associate II (Method Validation)
Covance, Inc.
01.2019 - 01.2021
- Oversaw the Discovery Program, establishing analytical method development for new client contracts.
- Performed analytical assessment of drug candidates in accordance with GLP guidelines.
- Conducted development and validation of LC-MS/MS mass spectrometry methods.
- Worked to expedite established bioanalytical methods through the incorporation of advanced liquid-handling instrumentation (Hamilton Star method development and programming).
- Interpreted laboratory results to provide technical advice to scientists.
- Troubleshot and resolved equipment and process issues.
Postdoctoral Scholar
University of Utah
01.2016 - 01.2019
- Applied bioconjugation strategies towards the development of modified deoxyribozymes (DNAzymes) with improved catalytic activity.
- Developed threose nucleic acid (TNA) biosensors for recognition and/or quantification of small molecule toxins.
- Performed recombinant protein expression of engineered polymerases for primer extension of TNA oligonucleotides.
- Leveraged interpersonal and communication skills to mentor PhD, graduate and undergraduate students.
- Designed research projects and alternative approaches and discussed results with postdoctoral supervisor
- Participated in professional development opportunities to stay current with industry trends and best practices.
Postgraduate Researcher
Monash University
01.2012 - 01.2016
- Designed and executed structure-activity relationship (SAR) studies of neuroactive peptides to identify important residues for voltage-gated sodium channel (VGSC) blockade
- Synthesized peptide analogs with enhanced pharmacological activities.
- Performed structural characterization of peptide analogs using one-dimensional and two-dimensional NMR.
- Assessed pharmacological activities of modified peptide analogues using two-electrode voltage clamp electrophysiology.
- Applied recombinant protein expression for large-scale production of peptide analogs for SAR studies.
- Performed solid-phase peptide synthesis (SPPS) and RP-HPLC purification of analgesic peptide analogs.
- Increased knowledge dissemination by presenting research findings at conferences, workshops, and seminars.
- Published research articles in peer-reviewed journals, contributing to the advancement of the field.
Research Associate
University of Utah
01.2004 - 01.2012
- Performed solid- and solution-phase peptide synthesis and RP-HPLC purification of first-in-class therapeutics for the treatment of pain and epilepsy.
- Coordinated efforts between the University of Utah and Johnson & Johnson PRD for the testing and characterization of venom-derived peptides.
- Performed structural characterization of modified analogues by circular dichroism (CD) spectroscopy.
- Developed in vitro assays to assess metabolic stability, bioavailability and receptor-binding affinity.
- Conducted structure-activity relationship (SAR) studies to identify amino acid residues responsible for the biological activity of peptide analogues
- Compared peptide analogues by 1H NMR and developed of methods to assess the improved in vitro pharmacology.
- Collaborated with multidisciplinary teams to drive project success and achieve objectives.
- Coauthored manuscripts submitted for publication in peer-reviewed journals which further established credibility within our field.
Research Associate I
Cognetix, Inc.
01.2002 - 01.2004
- Designed peptide analogues and performed solid-phase peptide synthesis and RP-HPLC purification for potential analgesic drug development.
- Explored various oxidative folding strategies and cyclization methods for disulfide-rich peptides.
- Performed characterization and mass confirmation of synthesized peptides by MALDI-TOF MS.
- Prepared scientific reports and presentations summarizing research activities.
- Operated and performed preventative maintenance on laboratory equipment and instruments.
Education
Ph.D. - Medicinal Chemistry and Drug Action
Monash University
Melbourne, Australia01-2016
Master of Science - Medicinal Chemistry
University of Utah
Salt Lake City, UT01-2010
Bachelor of Science - Microbiology
Weber State University
Ogden, UT01-2006
Skills
- Solid Phase Peptide Synthesis (SPPS)
- Analytical and preparative RP-HPLC
- Two-electrode voltage clamp electrophysiology
- Nuclear Magnetic Resonance (NMR) spectroscopy
- LCMS and LCMS/MS
- MALDI-TOF mass spectrometry
- Circular Dichroism (CD) spectroscopy
- Polymerase Chain Reaction (PCR)
- Gel Electrophoresis
- Recombinant protein expression
- Scientific writing
- Training and mentoring
Affiliations
- American Society for Biochemistry and Molecular Biology (ASBMB)
- American Peptide Society (APS)
- European Peptide Society (EPS)
Communications And Presentations
- Towards modified TNA aptamers as high-affinity binders for Ochratoxin A, Green, B. R. and Heemstra, J. M., 2017, Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides, Newport, RI
- Towards modified TNA aptamers as high-affinity binders for Ochratoxin A, Green, B. R. and Heemstra, J. M., 2017, Molecular Assembly & Function Mini-conference, Atlanta, GA
- Solution structure of a derivative of Conotoxin µO-GVIIJ, a Recently-discovered Inhibitor of Voltage-gated Sodium Channels, Green, B. R., 2014, Peptide Users Group Winter Symposium, Melbourne, AUS
- Interactions of Disulfide-deficient Selenocysteine Analogues of µ-Conotoxin BuIIIB with the Voltage-gated Sodium Channel NaV1.3, Green, B. R., Zhang, M. M., Chhabra, S., Robinson, S. D., Bulaj, G. Norton, R. S., 2014, 39th Lorne Conference on Protein Structure and Function, Lorne, AUS
- Structural Determinants of Interactions Between µ-Conotoxin BuIIIB and NaV1.3, Green, B. R., Zhang, M. M., Olivera, B. M., Bulaj, G., Norton, R. S., 2013, 23rd American Peptide Symposium, Hawaii, HI
- Structural Determinants of Interactions Between µ-Conotoxin BuIIIB and NaV1.3, Green, B. R., Zhang, M. M., Olivera, B. M., Bulaj, G., Norton, R. S., 2012, 38th Lorne Conference on Protein Structure and Function, Lorne, AUS
- Is lipidization-cationization a broadly applicable method to improve penetration of neuropeptides across the blood-brain barrier?, Green, B. R., White, H. S. and Bulaj, G., 2011, 22nd American Peptide Symposium, San Diego, CA
Awards
- ASEE IPERF Fellowship, 01/01/21 - 12/31/22
- Australian Postgraduate Award and International Postgraduate Research Scholarship, 01/01/12 - 12/31/15
- Early Career Researcher Presentation Melbourne Protein Users Group Winter Symposium, 01/01/14
- 23rd American Peptide Symposium Aapptec Travel Award, 01/01/13
- 38th Lorne Conference on Protein Structure and Function Poster Prize Recipient, 01/01/12
- Graduated cum laude, Weber State University, 01/01/06
- Undergraduate Research Opportunities Program (UROP) mini-grant recipient, 01/01/02
Publications
- McMahon KL, O’Brien H, Schroeder CI, Deuis JR, Venkatachalam D, Huang D, Green BR, Bandyopadhyay PK, Yandell M, Safavi-Hemami H, Olivera BM, Vetter I and Robinson SD., 2023, Cell Mol Life Sci., 80(287), 1-13
- Green, BR and Olivera, BM, 2016, Curr Top Membr., 78, 65-86
- Green BR, Gajewiak J, Chhabra S, Skalicky J, Zhang MM, Bulaj G, Olivera BM, Yoshikami D, Norton RS., 2016, J Biol Chem., 291(13), 7205-20
- Green BR, Bulaj G, and Norton RS., 2014, Future Med Chem., 6(15), 1677-98
- Green BR, Zhang MM, Chhabra S, Robinson SD, Wilson MJ, Redding A, Olivera BM, Yoshikami D, Bulaj G, Norton RS., 2014, FEBS J., 281(13), 2885-98
- Gajewiak J, Azam L, Imperial J, Walewska A, Green BR, Bandyopadhyay PK, Raghuraman S, Ueberheide B, Bern M, Zhou HM, Minassian NA, Hagan RH, Flinspach M, Liu Y, Bulaj G, Wickenden AD, Olivera BM, Yoshikami D, Zhang MM., 2014, Proc Natl Acad Sci USA., 111(7), 2758-63
- Green BR, Klein BD, Lee HK, Smith MD, Steve White H, Bulaj G., 2013, Bioorg Med Chem., 21(1), 303-10
- Khoo KK, Gupta K, Green BR, Zhang MM, Watkins M, Olivera BM, Balaram P, Yoshikami D, Bulaj G, Norton RS., 2012, Biochemistry, 51(49), 9826-35
- Platt RJ, Han TS, Green BR, Smith MD, Skalicky J, Gruszczynski P, White HS, Olivera B, Bulaj G, Gajewiak J., 2012, J Biol Chem., 287(24), 20727-36
- Green BR, Smith M, White KL, White HS, Bulaj G., 2011, ACS Chem Neurosci., 2(1), 51-6
- Green BR, White KL, McDougle DR, Zhang L, Klein B, Scholl EA, Pruess TH, White HS, Bulaj G., 2010, J Pept Sci., 16(9), 486-95
- Robertson CR, Scholl EA, Pruess TH, Green BR, White HS, Bulaj G., 2010, J Med Chem., 53(4), 1871-5
- Holford M, Zhang MM, Gowd KH, Azam L, Green BR, Watkins M, Ownby JP, Yoshikami D, Bulaj G, Olivera BM., 2009, Toxicon, 53(1), 90-8
- White HS, Scholl EA, Klein BD, Flynn SP, Pruess TH, Green BR, Zhang L, Bulaj G., 2009, Neurotherapeutics, 6(2), 372-80
- Zhang L, Robertson CR, Green BR, Pruess TH, White HS, Bulaj G., 2009, J Med Chem., 52(5), 1310-6
- Bulaj G, Green BR, Lee HK, Robertson CR, White K, Zhang L, Sochanska M, Flynn SP, Scholl EA, Pruess TH, Smith MD, White HS., 2008, J Med Chem., 51(24), 8038-47
- Zhang MM, Green BR, Catlin P, Fiedler B, Azam L, Chadwick A, Terlau H, McArthur JR, French RJ, Gulyas J, Rivier JE, Smith BJ, Norton RS, Olivera BM, Yoshikami D, Bulaj G., 2007, J Biol Chem., 282(42), 30699-706
- Green BR, Catlin P, Zhang MM, Fiedler B, Bayudan W, Morrison A, Norton RS, Smith BJ, Yoshikami D, Olivera BM, Bulaj G., 2007, Chem Biol., 14(4), 399-407
- Buczek O, Green BR, Bulaj G., 2007, Biopolymers, 88(1), 8-19
- Bulaj G, Zhang MM, Green BR, Fiedler B, Layer RT, Wei S, Nielsen JS, Low SJ, Klein BD, Wagstaff JD, Chicoine L, Harty TP, Terlau H, Yoshikami D, Olivera BM., 2006, Biochemistry, 45(23), 7404-14
- Zhang MM, Fiedler B, Green BR, Catlin P, Watkins M, Garrett JE, Smith BJ, Yoshikami D, Olivera BM, Bulaj G., 2006, Biochemistry, 45(11), 3723-32
- Green BR, Bulaj G., 2006, Protein Pept Lett., 13(1), 67-70
- Fuller E, Green BR, Catlin P, Buczek O, Nielsen JS, Olivera BM, Bulaj G., 2005, FEBS J., 272(7), 1727-38
Languages
English
Native or Bilingual
Japanese
Full Professional
Spanish
Limited Working
Timeline
Senior Scientist
FAKnostics, LLC
01.2022 - Current
Research Fellow
Applied Biosensors, LLC
01.2021 - 01.2022
Research Associate II (Method Validation)
Covance, Inc.
01.2019 - 01.2021
Postdoctoral Scholar
University of Utah
01.2016 - 01.2019
Postgraduate Researcher
Monash University
01.2012 - 01.2016
Research Associate
University of Utah
01.2004 - 01.2012
Research Associate I
Cognetix, Inc.
01.2002 - 01.2004
Ph.D. - Medicinal Chemistry and Drug Action
Monash University
Master of Science - Medicinal Chemistry
University of Utah
Bachelor of Science - Microbiology
Weber State University
Similar Profiles
Kevin PenteltonKevin Pentelton
Delivery Driver/Dispatch Supervisor at Upstate Fulfillment LLC./UB Logistics LLC.Delivery Driver/Dispatch Supervisor at Upstate Fulfillment LLC./UB Logistics LLC.
Emma LuvianoEmma Luviano
Billing Manager at KDT LLC & KIDS DC, LLCBilling Manager at KDT LLC & KIDS DC, LLC
Kyle HornerKyle Horner
CSR & Logistics Coordinator at Global Plastics, LLC. - MGI International, LLC.CSR & Logistics Coordinator at Global Plastics, LLC. - MGI International, LLC.
Austin DeWittAustin DeWitt
Course Coordinator at Greenfield Lakes Golf CourseCourse Coordinator at Greenfield Lakes Golf Course
Rohan SrivastavaRohan Srivastava
Teacher's Assistant at Lucknow CollegeTeacher's Assistant at Lucknow College