Brett Seese
Chicago,IL
Summary
Scientist with a track record of innovation and precision, bringing over 8 years of expertise in recombinant protein engineering and production, process development, and quality control. Adept at configuring LIMS/ELN systems and utilizing advanced data analysis tools like SQL and Power BI to streamline workflows and enhance decision-making.
Overview
6
6
years of professional experience
1
1
Certification
Work History
Scientific Consultant
Lupagen
01.2025 - Current
- Independently established and maintained laboratory operations, managing procurement of reagents, biomaterials, and instrumentation to support uninterrupted, high-quality experimental workflows.
- Cultured and maintained mammalian cell lines under sterile conditions, supporting R&D initiatives in therapeutic development and ensuring reproducibility of client-facing experiments.
- Designed and executed flow cytometry assays to evaluate cellular uptake of investigational cell therapies, delivering actionable insights that informed formulation strategies and improved client outcomes.
- Applied principles of scientific consulting, translating complex technical findings into strategic recommendations, and aligning experimental design with client goals and regulatory expectations.
- Optimized lab resource planning and assay design, contributing to cost-effective, scalable solutions in fast-paced biotech environments.
Lab Operations Consultant
Karchem Consulting
02.2024 - 12.2024
- Collaborated with scientists to assess ELN needs and configure complex SaaS platforms such as Benchling, LabGuru, and LabWare, ensuring alignment with scientific workflows and regulatory requirements.
- Led SaaS implementation projects across biotech and pharmaceutical clients, applying the full Software Development Life Cycle (SDLC) to guide configurations—from requirements gathering and testing to deployment and ongoing support.
- Provided strategic consulting on lab informatics systems, advising clients on software selection, implementation best practices, and optimization to drive efficiency, compliance, and scalability.
- Prepared regulatory dossiers for cGMP labs, aligning documentation with FDA and global regulatory standards to ensure successful Pre-Approval Inspections (PAIs) and audit readiness.
- Utilized deep knowledge of ICH guidelines, quality systems, and regulatory expectations to help pharmaceutical and manufacturing clients maintain global compliance and adopt industry best practices.
- Managed change control documentation, drafting detailed impact assessments to track software updates and maintain validated states within regulated environments.
Research Associate
Evozyne
07.2021 - 08.2023
- Developed and optimized chromatography methods (including HPLC and GC) to enhance protein purification workflows, ensuring efficiency and compliance with GxP and regulatory standards.
- Designed and validated ELISA assays that reduced antibody and substrate usage by 30% and cut overall assay costs by 50%, improving lab efficiency and scalability.
- Contributed to formulation development for drug products, optimizing stability and efficacy in accordance with industry and regulatory expectations.
- Collaborated cross-functionally with research and automation teams to write Python-based scripts that streamlined lab operations (e.g., plasmid prep), improving reproducibility and throughput.
- Experienced in NGS library preparation and bioinformatics pipelines, including quality control, alignment, and data interpretation for applications in molecular biology and genomics.
Graduate Teaching Assistant
Buffalo State University
08.2019 - 08.2021
- Established and maintained diverse mammalian and insect cell lines, ensuring viability and reproducibility across multiple experimental platforms.
- Optimized lipid-based RNAi protocols to achieve efficient knockdown of SIMs, including CENP-6, SENPs, RNF4, and RANGAP, enabling targeted functional studies.
- Trained and supervised undergraduate researchers in aseptic technique, cell culture maintenance, and transfection procedures to ensure consistency and lab compliance.
- Utilized fluorescence microscopy to visualize GFP-tagged SIM interactions throughout the mammalian cell cycle, contributing to mechanistic insights into chromatin and protein dynamics.
Education
Masters of Science (M.S) - Molecular Biology
Buffalo State University
Buffalo, NY08.2021
Bachelor of Science (B.S) - Neuroscience
University Of Pittsburgh
Pittsburgh, PA05.2018
Skills
- Protein Purification
- PCR
- QPCR
- ELISA
- ADME
- HPLC-MS
- SEC
- Flow Cytometry
- RNA/DNA extraction
- CRISPR/Cas9
- Gibson Assembly
- Golden Gate Assembly
- Assay Development
- Transfection
- Data Analysis
- SQL
- SnapGene
- GraphPad
- Python
- PowerBI
- Mammalian Cell Culture
- LabWare
- LabVantage
- Benchling
- LabGuru
- Research design
- Statistical modeling
Certification
Benchling Certified Practitioner
Benchling Certified Administrator
Timeline
Scientific Consultant
Lupagen
01.2025 - Current
Lab Operations Consultant
Karchem Consulting
02.2024 - 12.2024
Research Associate
Evozyne
07.2021 - 08.2023
Graduate Teaching Assistant
Buffalo State University
08.2019 - 08.2021
Bachelor of Science (B.S) - Neuroscience
University Of Pittsburgh
Masters of Science (M.S) - Molecular Biology
Buffalo State University
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