CAROLYN BARBIE

Full-time immunology research intern conducting T-cell receptor mutagenesis projects

• Conducted experiment mutating residues around CDR3 Loop to increase expression of TCR without changing binding properties
• Directed project testing for cross-reactivity of TCR to mutated strands of Influenza A, focusing on PB-1 protein
• Actively participated in journal clubs and presentations to learn details of post doctoral projects and implications in the broader context of social medicine
• Ran site-directed mutagenesis (SDM) reactions, PCR, transformations, transfections, mini and maxi preps, NanoDrop spectrophotometry, analyzed gene sequences
• Gained tissue culture manual skills by passaging, splitting, and counting cells and trained to do mice technical work

• Brainstormed methods to make immunotherapies more cost-efficient while researching medical barriers to CAR T-cell therapy
• Assisted study using a novel platform to deliver CAR T-cell therapy to Triple Negative Breast Cancer
• Collected and analyzed data

• Analyzed and interpreted demographic data of international Triple Negative Breast Cancer patients in relation to socioeconomic disparities in living communities
• Assisted study of signal transduction pathways in breast cancer
• Gained fundamental technical skills such as western blotting, running BCA protein assays, manually developing film, maintaining cancer cell lines
• Deepened knowledge of cancer genetics and sociopolitical implications through weekly journal club

• Pioneered program granting 100+ senior citizens free individualized studio photoshoots and interviews to preserve their histories
• Photographed senior center's Supportive Day group for elders with cognitive and physical impairments, attending to their needs patiently and gently
• Created portfolios for each family and presented project at senior fall art gala, displayed images on the walls of the center to foster community connection
• Exhibited at the prestigious National University of Singapore Health and Longevity Series webinar. Link: https://youtu.be/HA7wu9Dzq58?si=M5p9WxtjFki8dFMl

• Notated and published original pop interpolation arrangements on MuseScore for aspiring musicians to download for free
• Trained pianist of 12 years at the Real School of Music
• Trained vocalist in folk, pop, and classical music
• Composed and presented educational rap, "Can we share this song? A 90s Hip-Hop Rewrite of the L.A. Riots History Through the Lens of Afro-Asian Solidarity” at symposium, “What is the state of American art,” organized by Lunder Institute at Addison Gallery of American art.
• Event brought together hundreds of prominent professors and curators from across New England

• Spearheaded weekly individualized studio photoshoots of 300+ students, promoting cultural pride and self-expression through premiere fashion magazine
• Recruited photographers, models, led board, and organized student response forms
• Doubled social media following by rebranding as lead manager and graphic designer of account
• Provided free portfolios for student government campaigns, student actors, and varsity teams
• Donated $2k to local non-profit children’s organizations by organizing Phillips Andover thrift shop each trimester

• Mentored and recruited junior photographers, wrote and sent assignment emails delegating tasks 2x/week
• Published 100+ photos of varsity sports, events, and fashion
• Organized and sorted photos in the drive daily
• Authored a photo guide for future generations of Phillipian photographers
• Strengthened journalistic writing skills through meetings to review redlined papers every Monday, ultimately publishing music review for Arts section

• Selected leader for yearlong program dedicated to integrating ten freshmen into a boarding-student dominated community
• Facilitated advisory conversations, activities, and meals 2x/week
• Established strong relationships with students, teachers, and fellow mentors, building open and supportive communication

• Taught freshmen about race, gender, sexuality, and wellbeing in a classroom setting for 45 minutes every week
• Navigated challenging and crucial discussions, reflections, and self-exploration activities
• Sharpened leadership skills at 2-hour trainings every other week
• Designed engaging curriculum adaptations tailored to students' learning styles.

• Photographed and edited 1000+ bicyclists crossing the finish line for Dana-Farber Cancer Institute's Pan-Mass Challenge to honor families of cancer patients and raise funds for cancer research

• Catalogued costumes across decades for the Andover Theater Department's novel organization system every weekday during winter term by writing cards, sorting clothing, and photographing inventory