Cemil Boyoglu

My current role of a Lead Scientist focuses on driving advancements and manage application roadmap projects from inception to completion in Cell and Gene Therapy department. I am currently part of cutting-edge research and development activities for cell culture media production mainly for CAR-T and MSC growth. My main responsibilities are including but not limited to:

§ Developing project plans, setting goals and objectives, allocating resources, and ensuring the project stays on track

§ Coordinating with cross-functional teams, including researchers, technicians, and engineers, to ensure effective collaboration and timely delivery of project milestones.

§ Designing and executing experiments, collecting and analyzing data, developing innovative solution to address scientific challenges and provide a guidance to commercial product development

§ Recruiting and training new team members, ensuring a competent and motivated workforce.

§ Collaborating with marketing and regulatory affairs teams to prepare and submit necessary documents for approvals, certifications, and patents

I was involved in the development of a new cancer treatment method that demonstrated clinical efficacy. My main responsibilities were:

§ Developing and implementing novel assays based on siRNA transfection in cancer treatment

§ Partnering with other project leaders to plan and drive project completion enhance the overall quality of the research

§ Advising on appropriate in vitro models and collaborating with other internal and external groups

§ Leading and training research associates and technicians

.§ Obtaining and interpreting data, troubleshooting, and making decisions to move projects forward..

I was involved in basic research, assay development and drug screening for different type diseases. My main responsibilities include but not limited to;

§ Designing, planning, and performing experiments in vitro with different type of cancerous cell lines.

§ Implementing and optimizing the assays for monitoring and evaluating activity of Antibody Drug Conjugates by operating flow cytometry.

§ Performing assays with high throughput content imaging platforms to determine antibody internalization and cellular receptor quantification with Operetta CLS.

§ Investigating and developing new methods and technologies for project advancement

§ Identifying, managing and troubleshooting with collaborators and preparing scientific reports.

My work focused on methods and product development in cell and gene therapy applications. My main responsibilities are;

§ Implementing a robust and reproducible process to accommodate small scale AAV production

§ Designing the plan and protocols, and executing formal method validation to demonstrate precision, accuracy and robustness of developed analytical methods.

§ Preparing method development reports and conduct assays in adherent or suspension platforms

§ Preparing and reviewing process development protocols, data analysis summaries, technical reports, and other relevant process documentation

My research focused on Gene Editing technology using Adenoassociated Viruses that involves preparation and characterization of recombinant constructs, developing new methods to increasing transfection yields and scaling up the titer of virus. Unfortuantely, we could not complete the project due to pandemic shutdown but my responsibilities were;

§ Generating high-quality protocols, amendments, and reports for Gene Editing Technology and AAV production.

§ Reviewing, interpreting, and presenting data on assigned studies, using the assistance of associate scientists and technicians.

§ Providing technical and scientific guidance to the research staff.

§ Attending scientific meetings, conferences and training courses to enhance job and professional skills.

§ Conducting hands on experiments including; culturing both adherent and suspension cell lines, molecular cloning, transfection, determining transfection efficiency operating FACS and Fluorescence Microscope, virus titration with using Q-PCR and virus purification etc

Performing all other related duties as assigned.

I have been a part of multi-disciplinary team working with diverse models and biological systems for target discovery, validation and product development in Next Generation Sequencing (NGS)

Technology. My main responsibilities were;

§ NGS library preparation

§ Analyzing large datasets from DNA and RNA sequences, and maintaining up-to-date developments in algorithms for analysis of analysis software, genotyping algorithms and variant

§ Designing and conducting hypothesis-based research and hands-on execution in partnership with bioinformaticians and a principal investigator

§ Operating with various programming languages and software packages including Phyton, CLC Genomics Workbench

During my project, I applied Adenovirus vectors to the development of genetically stable vaccines against pathogens in veterinary medicine.

§ Conducting hands-on molecular biology and cellular biology techniques including preparing and manipulating recombinant constructs, performing assays for restriction digestion analysis, designing primers, interpretation of sequencing dataset, operating vector mapping software such as VectorNTI, Snapgene and DNAstrider

§ Collaborating with lead scientist to develop novel techniques and experimental design, execution, data analysis and reports

§ Bringing projects from conceptions to product and collaborating and communicating with other scientific stakeholders and Department Homeland Security.

§ Leading a team of research associate and scientist completing assigned tasks and serving as a scientific mentor to overseeing laboratory experimentation and data analysis.

I have worked on a vaccine development for Respiratory Syncytial Virus (RSV). My main responsibilities were;

§ Designing and performing experiments to characterize T and B cell immune responses to therapeutics in animal models by ELISpot, ELISA, flow cytometry and Luminex

§ Implementing the aspects of projects by conducting various assays including culturing mammalian cells, growing and titrating virus, PBMC extraction from human blood samples, propagating viral stocks and preparing draft protocols and reports.

§ Analyzing data using appropriate analysis software, such as FlowJo and Excel

§ Comprehensively performing literature reviews to troubleshoot experiments and informing new avenues of research

§ Preparing presentations and present findings internally to diverse groups of researchers and management