Chad Artman

• Sterilely inoculated several hundred bacterial agar plates conducting growth viability assays
• Maintained bacterial cultures for biofilm outgrowth
• Prepared and tested bacterial samples during confocal microscopy
• Worked on several porcine burn wound infection studies
• Worked with sterilely isolated pigs to assess efficacy of Norovirus vaccines
• Recombinantly produced Salmonella protein for downstream analysis using FPLC
• Presented current work at several retreats and conferences

• Packaged food-grade gummies in GMP facility
• Promoted to Associate II and completed GMP qualification
• GMP documentation and work with QA
• Collaborated on several teams for interdepartmental projects

Managed lab inventory/logistics and oversaw vendor purchasing while remaining within budget constrictions

• Led research projects to investigate microbial interactions and their effects on human health.
• Mentored junior staff in best practices for data collection, analysis, and interpretation of results.
• Collaborated with cross-functional teams to integrate findings into product development initiatives.
• Cell culture of Vero, HT-29, HeLa for use in BSL-2 bacterial adhesion inhibition assay
• Performed reverse immunoprecipitation protocol to affinity purify batch-scale amounts of target antibodies
• Facilitated scientific communication with Senior Professor and Post-Doc researcher at UVA to design 3-stage mouse study; formulated and qualified chicken antibodies for use in said study
• Processed fecal samples from mouse study and adapted standard ELISA protocol to assay for residual chicken antibodies
• Developed commercial chicken antibodies production regarding efficient large-scale vaccinations, extraction, and related logistical challenges
• Organized lab equipment ergonomically; maintained proper calibration of equipment and kept lab space clean and functional

• Authored Covid-19 passive prophylactic paper: (https://doi.org/10.1371/journal.pone.0252399) and Authored two other papers involving ETEC, and NoroVirus directed IgY molecules: (https://doi.org/10.3390/v14112371)
• Sterilely produced hundreds of agar bacteria plates/maintained plates of BSL-2 organisms
• Established/Executed Standard Operating Procedures for antigen/adjuvant emulsion vaccines
• Conducted hundreds of antibody recoveries, QA via protein assay via BCA and UV-Spec, and traditional ELISA
• Performed hundreds of ELISA-based immunological assays for downstream antibody evaluation
• Established SOP for SDS-PAGE, Western Blot, and Dot-Blot using limited reagents
• Established and performed multi-well plate growth SOP with ETEC bacteria
• Routinely interacted and coordinated research with academic and professional collaborators including: ProDigest, JHU, and University of Northern Illinois

• Experience with fermentation and bacterial cell cultivation
• Appropriate lab techniques for live cell handling
• Use and knowledge of bioreactor system and processes
• Introduced to GMP and related quality assurance protocols required for high fidelity biologic production

• Conducted biological analytics including SDS-Page, Western Blotting, and Bradford Assays for department
• Semi-quantitative analysis via Densitometry and ImageJ Software
• DNA purification and Agarose gel analysis
• Operated autonomously with minimal supervision