Summary
Overview
Work History
Education
Skills
Accomplishments
Affiliations
Awards
Teaching experience
Websites
Timeline
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Chandrima Gain

Los Angeles,CA

Summary

Highly skilled Postdoctoral Researcher with extensive experience in biomedical research. Recognized for innovative approaches, analytical problem-solving skills, and deep understanding of scientific methodologies. Made significant contributions to groundbreaking studies in the field. Demonstrated exceptional communication skills and a collaborative spirit while effectively working in diverse teams.

Overview

13
13
years of professional experience

Work History

Postdoctoral Researcher

University of California, Los Angeles
Los Angeles, California
07.2022 - Current
  • Investigating molecular mechanisms of autophagy in lipid metabolism alongside Dr. Rajat Singh.

Postdoctoral Researcher

University of California, Los Angeles
Los Angeles, California
10.2022 - 06.2024
  • Contributed to cutting-edge infectious disease research on RSV and SARS-CoV at the David Geffen School of Medicine, UCLA as a Postdoctoral Research Scholar in Dr. Theodoros Kelesidis's Lab.

MSc Dissertation

University of Delhi, Department of Biochemistry
Delhi
01.2024 - 05.2024
  • Conducted research on the study of F-Virosome and their interaction with liver cells under the guidance of Dr. D.P. Sarkar at Delhi University, South Campus.

Phd

Presidency University, Kolkata
Kolkata, West Bengal
12.2016 - 05.2022
  • Conducted extensive research under the guidance of Dr. Abhik Saha on 'Crosstalk between Proteasomal and Lysosomal Protein Degradation Pathway in EBV-Induced B-cell Lymphomas' at Presidency University, Kolkata.

Summer Intern

IISC
Bangalore, Karnataka
06.2011 - 08.2011
  • Summer trainee at IISC, Bangalore under the supervision of Dr. Karthik Sanker's laboratory.

Education

Master of Science - Biotechnology

St. Xavier's College, Kolkata
Kolkata, India
05-2015

Skills

  • Working experience in cancer biology
  • Mammalian cell culture maintenance (adherent, suspension) including cancer and virus producing cell lines
  • Nucleic acid purification and Western blot analysis
  • Expertise in Reverse transcription quantitative PCR (RT-qPCR)- Real Time Array and Real Time PCR
  • Proficiency in confocal microscopy
  • Immunoprecipitation (IP), Chromatin immunoprecipitation (ChIP)- sequence data analysis (BAM files) by IGV software
  • Carrying out flow cytometric analysis (using FlowJo software)
  • Strong experience with basic micro bacterial culture including molecular cloning and expression of proteins, and over-expression of recombinant protein in bacteria on IPTG induction
  • Good working knowledge on cell based assays- proliferation, cell viability and sub-cellular fractionation and in vitro assays (migration, colony formation),
  • Cytotoxicity assay- LDH assay and MTT assay
  • Expertise on Intracellular immunosorbent assay (EIA)
  • Generation of lentivirus-based over-expression and knockdown cell lines of constitutive, transient or inducible nature for overexpressing or silencing the gene of interest
  • si-RNA and CRISPR mediated knockdown
  • RNA-Seq data analysis using different software
  • Assisting in Digital Droplet PCR
  • Strong experience with scientific writings and progress report development
  • TCGA database analysis
  • Skilled in CorelDRAW
  • Expertise in data analysis using GraphPad Prism
  • Working with basic Virology techniques at BSL2 lab
  • Air-Liquid Interface (ALI) culture method: in vitro 3D cell culture method
  • Trained for BSL3 lab practice
  • Mice handling certified training
  • Proficient Tail vein injection, Intraperitoneal (IP) Injection, Subcutaneous injection
  • Experienced in Liver research
  • Monitoring animal protocol for refinement of experimental animal use

Accomplishments

  • D Das, M Sharma, D Gahlot, S S. Nia, C Gain, M Mecklenburg, Z. Hong Zhou, M Bourdenx, LThukral, N Martinez-Lopez, R Singh.VPS4A is the selective receptor for lipophagy in mice and humans (under review).
  • A Kombe, L Fotoohabadi, R Nanduri,Y Gerasimova ,M Daskou, C Gain, E Sharma, M Wong and T Kelesidis. The Role of the Nrf2 Pathway in Airway Tissue Damage Due to Viral Respiratory Infections. (2024).Int. J. Mol. Sci. 2024
  • S Malik, J Biswas, P Sarkar, S Nag, C Gain, S Ghosh Roy, B Bhattacharya, D Ghosh, A Saha. Differential carbonic anhydrase activities control EBV-induced B-cell transformation and lytic cycle reactivation. (2024) PLoS Pathogens 20 (3), e1011998
  • M Wong, C Gain, MB Sharma, L Fotooh Abadi, C Hugo, H Vassilopoulos,M Daskou, G Fishbein, Todoros Kelesidis. (2024). Severe Acute Respiratory Syndrome Coronavirus 2 Infection Alters Mediators of Lung Tissue Remodeling In Vitro and In VivThe Journal of Infectious Diseases 229 (5), 1372-1381 2024
  • M Daskou, L Fotooh Abadi, C Gain, M Wong, E Sharma, A Kombe Kombe, R Nanduri, T Kelesidis. (2024)The Role of the NRF2 Pathway in the Pathogenesis of Viral Respiratory Infections, Pathogens 13 (1), 3932024
  • Gain C, Song S, Angtuaco T, Satta S, Kelesidis T (2023). The Role of Oxidative Stress in the Pathogenesis of Infections with Coronaviruses" submitted to Frontiers in microbiology 13, 1111930
  • Gain C, Malik S, Bhattacharjee S, Ghosh A Robertson E S., Saha A (2020). Proteasomal Inhibition Triggers Viral Oncoprotein Degradation via Autophagy-Lysosomal Pathway. Plos Pathogens, DOI.org/10.1101/780171.
  • Gain C, Sarkar A, Bural S, Rakshit M, Banerjee J, Dey A, Biswas N, Kar GK, Saha A. (2021) Identification of two novel thiophene analogues as inducers of autophagy mediated cell death in breast cancer cells. Bioorg Med Chem. DOI: 10.1016/j.bmc.2021.116112.
  • Bhattacharjee S, Bose P, Patel K, Ghosh Roy S, Gain C, Gowda H, Robertson E S., Saha A (2018). Transcriptional and epigenetic modulation of autophagy promotes EBV oncoprotein EBNA3C induced B-cell survival. Cell Death and Disease, 9:605. DOI: 10.1038/s41419-018-0668-9

Affiliations

  • Gain C, Malik S, Bhattacharjee S, Ghosh A, Robertson ES, Das BB, Saha A. "Proteasomal Inhibition Triggers Viral Oncoprotein Degradation via Autophagy-Lysosomal Pathway". ePoster presentation in Keystone Symposia eSymposia 2021, on Targeted Protein Degradation: From Small Molecules to Complex Organelles|EK40 from June 7-8, 2021.
  • Gain C, Malik S, Bhattacharjee S, Ghosh A, Robertson ES, Das BB, Saha A. "Proteasomal Inhibition Triggers Viral Oncoprotein Degradation via Autophagy-Lysosomal Pathway". Poster presentation in International Conference on Autophagy and Lysosomes 2020 from January 16-18, 2020 at IISC, Bengaluru.
  • Gain C, Malik S, Bhattacharjee S, Ghosh A, Robertson ES, Das BB, Saha A. “Proteasomal Inhibition Enhances Viral Protein Degradation via Autophagy-Lysosomal Pathway.” Poster presentation in 6th Molecular Virology Meeting 2019 from 28th Feb- 2nd March, 2019 at School of Bioscience at IIT Kharagpur.
  • Bhattacharjee S, Bose P, Ghosh Roy S, Gain C, Saha A. “Epstein - Barr Virus Nuclear Antigen EBNA3C Transcriptionally Modulates Autophagy Network- A Clue to B- cell Lymphomagenesis”.
  • Poster presentation in ‘19th Transcription Assembly Meeting’ from November 8-9, 2016 at Bose Institute, Kolkata

Awards

  • UGC-JRF NET: Rank: 116; Result declared on March, 2016.
  • CSIR-JRF NET: Rank: 86; Result declared on October, 2016.
  • GATE: Score: 349; Percentile: 86.6; Result declared on March, 2016.
  • JGEEBILS-TIFR NCBS 2016: Reference code: 79321

Teaching experience

40hrs of practical class teaching experience to Master’s students.

Timeline

MSc Dissertation

University of Delhi, Department of Biochemistry
01.2024 - 05.2024

Postdoctoral Researcher

University of California, Los Angeles
10.2022 - 06.2024

Postdoctoral Researcher

University of California, Los Angeles
07.2022 - Current

Phd

Presidency University, Kolkata
12.2016 - 05.2022

Summer Intern

IISC
06.2011 - 08.2011

Master of Science - Biotechnology

St. Xavier's College, Kolkata
Chandrima Gain