CHARMI YOGI
Villa Rica,GA
Summary
Detail-oriented Biotechnology Student and Microbiology Graduate with expertise in laboratory techniques, microbial genetics, and aseptic practices. Proficient in culturing microorganisms, slide preparation, and biochemical testing. Developed strong research and analytical skills, with a proven ability to manage projects and analyze complex data. Seeking to leverage technical skills in a dynamic research or diagnostic lab environment.
Overview
1
1
year of professional experience
Work History
Student Research Assistant
Hassan Laboratory, Department of Dentistry, UAB
06.2025 - 12.2025
- Conducted cDNA synthesis to generate complementary DNA from RNA templates, supporting downstream cloning and expression studies.
- Performed plasmid isolation and purification and DNA precipitation to obtain high-quality DNA for mutagenesis and recombinant experiments.
- Applied site-directed mutagenesis techniques to introduce targeted sequence changes, enabling functional analysis of specific genes.
- Executed Polymerase Chain Reaction (PCR) and optimized conditions to amplify DNA fragments with accuracy and reproducibility.
- Carried out agarose gel electrophoresis to verify DNA amplification and assess nucleic acid integrity.
- Performed SDS-PAGE and Western blotting, including protein extraction, gel electrophoresis, membrane transfer, antibody incubation, and detection to analyze protein expression profiles.
- Conducted cell transfection using plasmid DNA to study gene expression and functional outcomes in mammalian cell systems.
- Executed bacterial transformation by introducing plasmid DNA into competent cells, followed by colony screening and verification of successful clones.
- Measured DNA and RNA concentration and purity using NanoDrop spectrophotometry to ensure sample quality for downstream applications.
- Prepared and validated experimental controls and master mixes to ensure accuracy and reproducibility of molecular biology assays.
- Documented, analyzed, and interpreted experimental results with strong attention to detail, ensuring experimental accuracy and reproducibility.
- Maintained strict adherence to biosafety, aseptic technique, and quality control protocols, supporting a safe and efficient laboratory environment.
Education
Master of Science - Biotechnology
UAB School of Health Professions
12.2025
Master of Science - Microbiology
Gokul Global University
Gujarat, India06.2024
Bachelor of Science - Microbiology
Ganpat University
Gujarat, India06.2022
Skills
- PCR
- Microbial Pathogen Detection
- Biochemical Assays
- Antibiotic Sensitivity Testing
- Genotyping
- Western Blotting
- Cell culture
- DNA & RNA Extraction
- Gel Electrophoresis
- Gram Staining
- Aseptic and Biosafety Practices
- Immunoassays & Disease Biomarkers
- Scientific methodology
- Research design
- Qualitative research
- Subject matter expertise
- Documentation expertise
- Lab techniques
- Clinical assays
- Data collection
- Teamwork
- Teamwork and collaboration
- Problem-solving
- Attention to detail
- Microsoft Excel proficiency
- Laboratory management
- Equipment maintenance
Languages
Fluent in English, Gujarati, Hindi
ADDITIONAL INFORMATION
- Certifications: Microsoft Office Suite, Research Data Interpretation
- Molecular Biology Techniques – PCR, DNA Isolation, Genotyping, Agarose Gel Electrophoresis
- Microbial & Biochemical Assays – ELISA, Hematology, Glucose & Protein Quantification, Antibiotic Sensitivity Testing
Timeline
Student Research Assistant
Hassan Laboratory, Department of Dentistry, UAB
06.2025 - 12.2025
Master of Science - Biotechnology
UAB School of Health Professions
Master of Science - Microbiology
Gokul Global University
Bachelor of Science - Microbiology
Ganpat University