Detail-oriented Biotechnology Student and Microbiology Graduate with expertise in laboratory techniques, microbial genetics, and aseptic practices. Proficient in culturing microorganisms, slide preparation, and biochemical testing. Developed strong research and analytical skills, with a proven ability to manage projects and analyze complex data. Seeking to leverage technical skills in a dynamic research or diagnostic lab environment.
Hi, I’m
CHARMI YOGI
Villa Rica,GA
Summary
Overview
1
year of professional experience
Work History
Hassan Laboratory, Department of Dentistry, UABBirmingham, AL
Student Research Assistant
06.2025 - 12.2025
Job overview
- Conducted cDNA synthesis to generate complementary DNA from RNA templates, supporting downstream cloning and expression studies.
- Performed plasmid isolation and purification and DNA precipitation to obtain high-quality DNA for mutagenesis and recombinant experiments.
- Applied site-directed mutagenesis techniques to introduce targeted sequence changes, enabling functional analysis of specific genes.
- Executed Polymerase Chain Reaction (PCR) and optimized conditions to amplify DNA fragments with accuracy and reproducibility.
- Carried out agarose gel electrophoresis to verify DNA amplification and assess nucleic acid integrity.
- Performed SDS-PAGE and Western blotting, including protein extraction, gel electrophoresis, membrane transfer, antibody incubation, and detection to analyze protein expression profiles.
- Conducted cell transfection using plasmid DNA to study gene expression and functional outcomes in mammalian cell systems.
- Executed bacterial transformation by introducing plasmid DNA into competent cells, followed by colony screening and verification of successful clones.
- Measured DNA and RNA concentration and purity using NanoDrop spectrophotometry to ensure sample quality for downstream applications.
- Prepared and validated experimental controls and master mixes to ensure accuracy and reproducibility of molecular biology assays.
- Documented, analyzed, and interpreted experimental results with strong attention to detail, ensuring experimental accuracy and reproducibility.
- Maintained strict adherence to biosafety, aseptic technique, and quality control protocols, supporting a safe and efficient laboratory environment.
Education
UAB School of Health Professions
Master of Science from Biotechnology
12.2025
University Overview
- 3.4 GPA
- Capstone Project: Completed a capstone project analyzing Merck’s Keytruda (pembrolizumab), focusing on its scientific mechanism, clinical development, regulatory approvals, safety profile, and real-world patient outcomes. Assessed market dynamics, competitive landscape, intellectual property strategy, revenue growth, and sustainability efforts, demonstrating an integrated understanding of biologic drug development from bench to market.
Gokul Global UniversityGujarat, India
Master of Science from Microbiology
06.2024
University Overview
- 3.2 GPA
- Capstone Project: Microbial Biotechnology in Industrial and Medical Application :
This topic focuses on the use of microorganisms such as bacteria, yeast, and fungi for the production of valuable biological products including antibiotics, vaccines, enzymes, and therapeutic proteins. It explores how microbial systems are engineered and optimized to improve yield, stability, and safety in industrial and medical settings. The study highlights applications of microbial biotechnology in pharmaceuticals, diagnostics, and sustainable manufacturing, emphasizing its importance in advancing healthcare innovation and bioprocess development.
Ganpat UniversityGujarat, India
Bachelor of Science from Microbiology
06.2022
University Overview
- 3.0 GPA
Skills
- PCR
- Microbial Pathogen Detection
- Biochemical Assays
- Antibiotic Sensitivity Testing
- Genotyping
- Western Blotting
- Cell culture
- DNA & RNA Extraction
- Gel Electrophoresis
- Gram Staining
- Aseptic and Biosafety Practices
- Immunoassays & Disease Biomarkers
- Scientific methodology
- Research design
- Qualitative research
- Subject matter expertise
- Documentation expertise
- Lab techniques
- Clinical assays
- Data collection
- Teamwork
- Teamwork and collaboration
- Problem-solving
- Attention to detail
- Microsoft Excel proficiency
- Laboratory management
- Equipment maintenance
Languages
Fluent in English, Gujarati, Hindi
ADDITIONAL INFORMATION
ADDITIONAL INFORMATION
- Certifications: Microsoft Office Suite, Research Data Interpretation
- Molecular Biology Techniques – PCR, DNA Isolation, Genotyping, Agarose Gel Electrophoresis
- Microbial & Biochemical Assays – ELISA, Hematology, Glucose & Protein Quantification, Antibiotic Sensitivity Testing
Timeline
Student Research Assistant
Hassan Laboratory, Department of Dentistry, UAB
06.2025 - 12.2025
UAB School of Health Professions
Master of Science from Biotechnology
Gokul Global University
Master of Science from Microbiology
Ganpat University
Bachelor of Science from Microbiology