Constanza Savid-Frontera
Tampa,FL
Summary
Experienced immunologist and cell therapy scientist with a strong background in molecular biology. Skilled in leading research projects, conducting complex experiments, and analyzing data. Proven expertise in project management, multi-parametric flow cytometry, in vitro assays, cell culture, and gene editing. Collaborates effectively with multidisciplinary teams and communicates findings to diverse audiences.
Overview
12
12
years of professional experience
Work History
Postodoctoral Fellow
H. Lee Moffitt Cancer Center & Research Institute
Tampa, USA
11.2020 - Current
- Postdoctoral research focused on improving persistence, fitness, and functionality of chimeric antigen receptor (CAR)-T cell therapies for treating hematologic malignancies.
- Independently coordinate, design, and execute research projects in the laboratory, performing cutting-edge techniques such as CRISPR/Cas9, RNA-seq, RT-qPCR, ELLA, multiparametric flow cytometry, real-time cell analysis, and in vivo imaging.
- Expertise with pre-clinical in vitro and in vivo potency and safety studies. Experience with humanized mouse models for FDA approval.
- Analyze scientific data, create figures, prepare, and present results at internal and external meetings.
- Mentor and train undergraduate and graduate students.
- Networking Chair of the Moffitt Postdoctoral Association (MPDA) (2023 to present), orchestrating engaging social and networking gatherings aimed at enhancing the professional growth of Moffitt Trainees.
Assistant Professor
Facultad de Ciencias Químicas, Universidad Nacional de Córdoba (UNC)
Córdoba, Argentina
03.2015 - 10.2020
- Class planning and preparation of slide decks.
- Lecturing, presentation of materials, and activities.
- Preparation and correction of exams, including review.
- Classroom engagement through communication and public speaking skills.
Research Graduate (PhD)
Centro de Investigaciones en Bioquimica Clinica e Inmunología (CIBICI)-CONICET- Universidad Nacional de Córdoba (UNC)
Córdoba, Argentina
04.2015 - 09.2020
- Analyzed complex scientific data, providing insights into cancer immunology and immunotherapy.
- Designed and performed in vitro, ex vivo, and in vivo experiments (murine models of cancer).
- Presented findings at internal and external (national and international) meetings and conferences, facilitating scientific exchange and knowledge dissemination.
Short Term internship
Inserm U1082, CHU de Poitiers, Universite de Poitiers
Poitiers, France
09.2019 - 12.2019
- To evaluate the role of Virtual memory CD8+ T cells on human hematologic malignancies
Visiting PHD Student
Inserm U1016, Institut Cochin
Paris, France
10.2018 - 12.2018
- Understanding the role of tumor-infiltrating virtual memory CD8+ T cells: study of their behavior inside solid tumors ex-vivo and their activation profile against cancer cells ex-vivo/in vitro
Ad honorem internship
Laboratory of Chronobiology at Departamento de Química Biológica- Facultad de Ciencias Químicas- Universidad Nacional de Córdoba- CIQUIBIC- CONICET
Córdoba, Argentina
08.2012 - 12.2012
- Ad honorem internship in the Laboratory of Chronobiology- Departamento de Química Biológica- Facultad de Ciencias Químicas- Universidad Nacional de Córdoba- CIQUIBIC- CONICET
Education
Research Graduate (PhD) - Study and characterization of innate CD8+ T cells as possible therapeutical mediators in murine cancer models and cancer patients.
Universidad Nacional de Córdoba (UNC)
Córdoba, Argentina09.2020
Biochemist -
Universidad Nacional De Córdoba
Córdoba, Argentina12.2014
Skills
- Project Management
- Multi-parametric Flow Cytometry
- ELISA
- ELLAs
- Cell culture
- In vitro functional assays
- CAR-T design and testing
- Retroviral transduction
- Lentiviral transduction
- CRISPR/Cas9 gene-editing
- In vivo tumor models
- DNA/RNA Extraction
- PCR
- RT-PCR
- Teaching experience
- Mentoring
Accomplishments
- 2024 Junior Scientist Research Partnership Award, Tampa, FL, October 2024
- Boehringer Ingelheim Fonds Travel Grant , Poitiers, France, September 2019 – December 2019
- The Company of Biologists- Disease Model and Mechanisms Travelling Fellowship (DMMTF), Paris, France, October 2018 – December 2018
- SAINT-EXUPÉRY FELLOWSHIP, Ministerio de Educación de La República Argentina- Campus France, MINISTÈRE DE L’EUROPE ET DES AFFAIRES ÉTRANGÈRES, RÉPUBLIQUE FRANÇAISE, Paris, France, October 2018 – December 2018
- Leonardo Satz Award for the best immunology work of the 2016, SAI Annual Meeting, Buenos Aires, Argentina, 2016
Contribution To Science
* Fortuna GG, Banerjee R, Savid-Frontera C*, et al. Immune effector cell-associated enterocolitis following chimeric antigen receptor T-cell therapy in multiple myeloma. Blood Cancer J. 2024;14(1):180. Published 2024 Oct 16. doi:10.1038/s41408-024-01167-8, , October 2024.
* Viano ME, Baez NS, Savid-Frontera C, et al. Systemic inflammatory Th1 cytokines during Trypanosoma cruzi infection disrupt the typical anatomical cell distribution and phenotypic/functional characteristics of various cell subsets within the thymus. Microbes Infect. 2024;26(5-6):105337. doi:10.1016/j.micinf.2024.105337, July 2024.
* Holtan SG, Savid-Frontera C*, Walton K, et al. Human Effectors of Acute and Chronic GVHD Overexpress CD83 and Predict Mortality. Clin Cancer Res. 2023 Mar 14;29(6):1114-1124. doi: 10.1158/1078-0432.CCR-22-2837. PMID: 36622700; PMCID: PMC10011883., March 2023.
* Viano ME, Baez NS, Savid-Frontera C, et al. Virtual Memory CD8+ T Cells: Origin and Beyond. J Interferon Cytokine Res. 2022;42(12):624-642. doi:10.1089/jir.2022.0053, , December 2022
* Goal, P, Kotani H, Yongliang Z, Reid K, Boucher JC, Savid-Frontera C, Lee SB, Beatty N, Jain MD, Davila ML; A Novel IL2Rα-/- Model for CAR-T Toxicity in Acute Lymphoblastic Leukemia Recapitulates Cytokine Release Syndrome and Neutropenia. Blood 2022; 140 (Supplement 1): 1176–1177. doi: https://doi.org/10.1182/blood-2022-165562, November 2022.
* Savid-Frontera C, Viano ME, Baez NS, et al. Exploring the immunomodulatory role of virtual memory CD8+ T cells: Role of IFN gamma in tumor growth control. Front Immunol. 2022;13:971001. Published 2022 Oct 18. doi:10.3389/fimmu.2022.971001, October 2022.
* Savid-Frontera C, Viano M, Baez N, Reynolds D, Matellon M, A Young H, Rodriguez-Galan M. Safety levels of systemic IL-12 induced by cDNA expression as a cancer therapeutic. Immunotherapy. 2021 November 16; :-. Available from: https://www.futuremedicine.com/doi/10.2217/imt-2021-0080 DOI: 10.2217/imt-2021-0080, November 2021.
* Baez N, Cerbán F, Savid-Frontera C, Hodge D, Tosello J, Acosta-Rodriguez E, Almada L, Gruppi A, Viano M, Young H, Rodriguez-Galan M. Thymic expression of IL-4 and IL-15 after systemic inflammatory or infectious Th1 disease processes induce the acquisition of "innate" characteristics during CD8+ T cell development. PLOS Pathogens. 2019; 15(1):e1007456-. Available from: https://dx.plos.org/10.1371/journal.ppat.1007456 DOI: 10.1371/journal.ppat.1007456, January 2019.
Timeline
Postodoctoral Fellow
H. Lee Moffitt Cancer Center & Research Institute
11.2020 - Current
Short Term internship
Inserm U1082, CHU de Poitiers, Universite de Poitiers
09.2019 - 12.2019
Visiting PHD Student
Inserm U1016, Institut Cochin
10.2018 - 12.2018
Research Graduate (PhD)
Centro de Investigaciones en Bioquimica Clinica e Inmunología (CIBICI)-CONICET- Universidad Nacional de Córdoba (UNC)
04.2015 - 09.2020
Assistant Professor
Facultad de Ciencias Químicas, Universidad Nacional de Córdoba (UNC)
03.2015 - 10.2020
Ad honorem internship
Laboratory of Chronobiology at Departamento de Química Biológica- Facultad de Ciencias Químicas- Universidad Nacional de Córdoba- CIQUIBIC- CONICET
08.2012 - 12.2012
Research Graduate (PhD) - Study and characterization of innate CD8+ T cells as possible therapeutical mediators in murine cancer models and cancer patients.
Universidad Nacional de Córdoba (UNC)
Biochemist -
Universidad Nacional De Córdoba
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