Cristobal Von Muhlenbrock
Watertown,MA
Summary
Motivated by a passion for innovation and a deep commitment to improving healthcare outcomes, this accomplished Biochemistry and Molecular Biology Pre-med graduate actively seeks a research opportunity. Possessing extensive hands-on experience in diverse laboratory techniques, protocols, and complex equipment operation, the goal is to contribute to groundbreaking research endeavors. The ultimate career objective is to be part of a team dedicated to developing innovative solutions that effectively address unmet medical needs, enhance patient outcomes, and have a positive impact on global healthcare. Demonstrating a diligent and independent work approach, complemented by a critical thinking mindset and strong problem-solving skills, the focus is on upholding scientific rigor and driving advancements in drug discovery, efficacy, and safety. By consistently expanding knowledge and staying informed about emerging developments, the commitment remains steadfast in making a meaningful and lasting impact on the lives of individuals and communities through transformative research.
Overview
11
11
years of professional experience
Work History
Lab Technician / Research Assistant
Boston Children's.hospital
07.2023 - Current
- Managed laboratory resources efficiently, ensuring optimal use of time and materials for successful project completion.
- Maintained detailed records of experimental procedures and results, facilitating reproducibility and future investigations.
- Optimized existing protocols for increased efficiency, saving time while maintaining data integrity.
- Collaborated with different investigators to drive projects to success and achieve objectives
- Collected, analyzed and interpreted information, documentation, and physical evidence associated with investigations.
- Performed procedures such as, Western Blots, PCR, gel electrophoresis, plasmid engineering, auditory brainstem responses, utricle viral injections in mice, dissections, immunostaining, imaging, DNA extractions, maxi-prep, mini-prep, cell culture maintenance, transduction, transfections, transformations, among many others.
Biochemistry Lab 426
University Of Massachusetts Amherst
09.2022 - 12.2022
- Conducted ongoing original research in biochemistry laboratory, advancing understanding of symbiotic interaction between legumes and rhizobia.
- Executed range of laboratory techniques including cell lysis, DNA extraction, Polymerase Chain Reactions (PCR), Golden Gate ligation.
Designed efficient primers targeting specific genomic regions for guide RNA (gRNA) synthesis. - Employed golden gate ligation to ligate gRNA sequences into plasmids, facilitating engineering of CRISPR/Cas9 construct.
- Performed transformation and electroporation of bacteria, followed by cloning.
- Implemented multiple verification procedures such as gel electrophoresis and NanoDrop analysis to ensure alignment with expected results.
- Maintained comprehensive documentation through diligent daily logs and equipment record books.
- Demonstrated expertise in statistical analysis, qualitative and quantitative data interpretation, and generating detailed reports.
- Implemented safety protocols and maintained clean, organized laboratory environment.
Research Assistant in the Muscle Biology Lab
UMass Amherst Kinesiology Department
05.2021 - 07.2022
- Worked independently and collaboratively in a fast-paced laboratory environment, contributing to research projects.
- Actively participated in seminars and workshops to stay updated with advancements in the field.
- Conducted statistical, qualitative, and quantitative analysis for data interpretation.
- Planned, modified, and executed research techniques, procedures, and tests with precision.
- Assembled 600+ T-clips weekly under a microscope for optimal skeletal muscle fiber preparation.
- Performed Oil Red O procedures to stain and analyze muscle fiber lipids (adipocytes).
- Visualized, quantified, and analyzed lipid droplets in muscle fibers using a light microscope.
- Generated comprehensive reports summarizing experimental procedures, data analysis, and key findings.
- Utilized effective scientific writing skills to clearly communicate research outcomes and conclusions
- reviewed and refine reports, ensuring accuracy and clarity.
- Presented research findings in internal meetings and conferences, demonstrating strong communication and presentation skills.
Organich Chemistry Lab
UMass Amherst
09.2020 - 12.2020
- Executed organic chemistry techniques: distillation, extraction, and recrystallization for compound separation and purification.
- Applied synthetic techniques: nucleophilic substitution, elimination, and addition reactions for organic compound transformation.
- Proficiently utilized chromatography techniques: column and TLC for organic compound separation and identification.
- Analyzed and characterized organic molecules using spectroscopic techniques: NMR, IR.
- Recorded precise experimental procedures, observations, and results in meticulous laboratory notebooks.
- Collaborated with lab partners, communicating findings effectively during discussions and presentations.
- Troubleshot experimental issues, optimizing reaction conditions for accurate results.
- Maintained strict adherence to laboratory safety protocols, promoting a clean and organized work environment.
Customs Agency Secretary
Luis Rafael Rodriguez Viancos Agencia De Aduanas
06.2016 - 09.2016
- Developed and updated Microsoft Excell spreadsheets and databases to track, analyze and report on performance and sales data
- Delivered top-notch administrative support to office staff, promoting excellence in office operations
- Facilitated efficient communication by promptly responding to emails and other correspondence.
- Organized envelopes, postage and mail correspondence for staff and management, maintaining postage meter and coordinating with delivery and courier services
- Managed approximately 30+ incoming calls, emails and faxes per day from customers
Brewer
Industria Cervecera Del Norte
07.2013 - 09.2013
- Brewed and delivered 2000 liters of beer weekly, equivalent to approximately 6000 350cc bottles.
- Conducted comprehensive quality checks by sampling each production and comparing it to specifications.
- Monitored and maintained batch quality through specific gravity and pH measurements.
- Accurately recorded and documented crucial data including temperature, flow, and other metrics.
- Ensured hygienic and efficient operations by regularly cleaning production lines and storage containers.
- Demonstrated commitment to quality assurance by reviewing data for compliance with specifications and company standards.
- Safely operated and meticulously sanitized all equipment to meet industry regulations.
- Efficiently managed stockroom and warehouse operations.
Education
Bachelor of Science - Biochemistry And Molecular Biology
University Of Massachusetts Amherst
Amherst, MA05.2023
High School Diploma -
The Cambridge School of Weston
Weston, MA06.2018
Skills
- Team work
- Adaptability and fast learning
- Data gathering and organization
- Safety awareness
- Safe handling of laboratory equipment
- Accurate measurements
- Good manufacturing practices
- Record-keeping abilities
- Precision
- Outgoing and energetic
- Critical thinking and problem solving
- Equipment maintenance
Community Service
- Rotary Club in Chile (Rotaplast): closely worked as a translator for American surgeons performing charity surgeries for people with cleft lip, microtia, anotia and breast cancer. ( 8 hrs/day, 5 days/week, during the month of February, years 2015-2017)
- Farming: Actively harvested vegetables for local farmers. (3hrs/ day, 1 day/ week, 2019)
- Active Buddies: specifically assisted handicap people to swim and have a good time at the Boston's sports club. (1 day/week, years 2017-2019)
- Sandwich Making: make sandwiches in order to give them to people who have mental illnesses. (2018-2019)
- Handed out groceries to people in need to make sure their primary needs were fulfilled. (2018-2019)
Other Interests
- Nutrition
- Health and Fitness
- Medicine
Languages
Spanish
English
French
Timeline
Lab Technician / Research Assistant
Boston Children's.hospital
07.2023 - Current
Biochemistry Lab 426
University Of Massachusetts Amherst
09.2022 - 12.2022
Research Assistant in the Muscle Biology Lab
UMass Amherst Kinesiology Department
05.2021 - 07.2022
Organich Chemistry Lab
UMass Amherst
09.2020 - 12.2020
Customs Agency Secretary
Luis Rafael Rodriguez Viancos Agencia De Aduanas
06.2016 - 09.2016
Brewer
Industria Cervecera Del Norte
07.2013 - 09.2013
Bachelor of Science - Biochemistry And Molecular Biology
University Of Massachusetts Amherst
High School Diploma -
The Cambridge School of Weston
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