Curt Bautista

• Ensured a 99% sterile compliance rate in surgical procedures by implementing rigorous sterile protocols, reducing post-procedure infection risks.
• Maintained contamination-free conditions by handling and processing 40+ biological specimens (blood, tissue) per week, following strict laboratory protocols.
• Assisted in specimen collection, preparation, and handling for over 1000 patient tests, adhering to strict sterile techniques and contamination prevention protocols.
• Experienced in maintaining accurate patient records of 500+ patients using electronic health record (EHR) systems, ensuring 100% compliance with HIPPA and data integrity standards
• Managed medical inventory, ensuring timely restocking of supplies and maintaining an organized work environment
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• Fostered interdisciplinary teamwork, coordinating patient care with a team of three healthcare professionals, improving procedural efficiency and patient satisfaction.

Thesis: Investigating the function of the AP-1 transcription factor Fosl1 in mammalian cardiomyocyte proliferation

• Conducted molecular cloning and genetic engineering using Multisite Gateway to generate adenoviral expression constructs
• Cultured and maintained primary neonatal rat cardiomyocytes, performing adenoviral transduction for gene overexpression.
• Conducted immunocytochemistry (ICC) and immunohistochemistry (IHC) for protein detection and cell cycle analysis.
• Performed PCR, nested PCR, and quantitative PCR (qPCR) for gene amplification and expression analysis.
• Analyzed RNA-seq data using DESeq2 to evaluate differential gene expression.
• Conducted sequence alignment, primer design, and plasmid verification using BLAST and Benchling.
• Performed image quantification and cell analysis using ImageJ/FIJI.
• Applied GraphPad Prism for statistical analysis of experimental data.
• Maintained electronic lab notebooks (ELN) for accurate research documentation.
• Designed and optimized experimental workflows to investigate transcription factor regulation in cardiac regeneration.
• Prepared and presented research findings in departmental meetings and academic conferences.

• Developed and delivered engaging molecular biology curricula, enhancing student comprehension PCR, gel electrophoresis, protein purification/quantification, bioinformatics, aseptic techniques, microscopy, and cell culture for over 20 students.
• Developed course materials and instructed undergraduate courses in Molecular Biology, Bacteriology, and Principles of Biology, emphasizing hands-on laboratory techniques and critical thinking
• Meticulously managed lab operations, ensuring strict adherence to scientific protocols and safety standards while mentoring students in scientific methodology/writing, experimental design, data analysis, critical analysis, and research techniques.
• Partnered with faculty and researchers to refine laboratory coursework, integrating cutting-edge laboratory techniques and fostering a dynamic learning environment.
• Contributed insights to curriculum development in departmental meetings, enhancing the quality of laboratory instruction and student learning outcomes.
• Prepared and graded comprehensive assessments, evaluating student grasp of biological research methods. Balanced teaching, grading, and research support effectively.
• Crafted and evaluated comprehensive assessments, driving measurable improvements in students' grasp of biological research methodologies.