Summary
Overview
Work History
Education
Skills
Internships And Training
Presentations
Publications
Timeline
Generic

Danielle Simning

Scientist
Gainesville,FL

Summary

Skilled Biological Scientist focused on aquatic toxicology. Exceptional research skills with demonstrated success in study design and execution. More than 10 years of environmental research experience in academia and private industry. Well-organized and enthusiastic with good communication and problem-solving skills.

Overview

14
14
years of professional experience

Work History

Raw Materials Facility Manager

NextGen Biologics
Alachua, FL
08.2024 - Current
  • Directed laboratory operations, aligning workflows with quality standards and project priorities.
  • Managed equipment calibration, preventive maintenance, and documentation for reliable lab performance.
  • Implemented SOPs to maintain compliance with GMP and audit requirements.
  • Monitored inventory levels, ordered critical supplies, and reduced workflow interruptions.
  • Investigated deviations and corrective actions to strengthen process consistency and data integrity using QT9 software.
  • Interviewed, hired and trained lab technicians to perform experiments and analyze results.

Quality Control Scientist II

Thermo Fisher Scientific Inc.
01.2022 - 03.2024
  • Evaluated components and final products against quality standards and manufacturing specifications using PCR methods.
  • Recorded and organized test data for report generation and analysis.
  • Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays or instrumentation.
  • Adhered to cGMP and ALCOA+ regulations

Research Scientist and Bioassay Lab Manager

NCASI Inc. Aquatic Biology
09.2017 - 09.2021
  • Maintained a research and bioassay aquatic testing facility in compliance with EPA, CEPA, and EEA regulations.
  • Supervised aquatic research assistants to ensure meeting project deadlines.
  • Interviewed, hired, and trained staff members for both aquatic and analytical chemistry laboratories.
  • Collaborated with government and industry leaders to assess potential limitations in approved regulatory water toxicity test methods.
  • Routinely performed water quality on effluent and environmental water samples to measure alkalinity, hardness, BOD, COD, conductivity, turbidity, and dissolved oxygen.
  • Performed HPLC and GC-MS methods to quantify concentrations of nutrients, heavy metals, and pesticides in water samples.
  • Identified issues, analyzed information, and provided solutions to problems related to pulp and paper effluent toxicity and environmental regulations problems.
  • Presented research findings annually at scientific conferences.

Graduate Research Assistant

Marine Toxicology Lab, USM-Gulf Coast Research Lab
01.2015 - 01.2017
  • Designed and executed multiple stressor aquatic exposures as part of a national resource damage assessment associated in response to the Deep-Water Horizon oil spill in 2020
  • Evaluated physiological stress responses of fish during early life development using molecular and phenotypic analysis
  • Conducted flow-through and static aquatic toxicity experiments
  • Collected, analyzed, and presented experimental data.
  • Enhanced research quality by conducting thorough literature reviews and synthesizing findings.
  • Performed DNA extractions, total RNA extractions, RNA integrity assessments, end-point PCR, quantitative PCR, gel electrophoresis, and designed primers for PCR analysis
  • Utilized advanced statistical software to analyze large RNA Sequencing datasets to uncover key insights for informed decision-making.

Research Technician

Marine Toxicology Lab, USM-Gulf Coast Research Lab
01.2013 - 01.2015
  • Assisted with field and laboratory studies assessing the impacts of oil contamination in estuarine fish, mollusks, and invertebrate species in the northern Gulf of Mexico.
  • Prepared oil exposure solutions for aquatic and sediment dose-response toxicity tests.
  • Performed routine water quality analysis in field and laboratory settings.
  • Completed monthly facility effluent analysis to remain compliant with local wastewater regulations.
  • Maintained multiple species of experimental animal brood stocks simultaneously to ensure project timeframes were met.
  • Demonstrated commitment to safe practices and good laboratory practices.
  • Kept accurate written records of testing and other data-gathering activities.

Education

Bachelor of Science - Marine Biology

The University of Southern Mississippi
Hattiesburg, MS

Master of Science - Ecotoxicology And Coastal Sciences

The University of Southern Mississippi
Gulfport, MS

Skills

  • Pharmaceutical Manufacturing
  • Aquatic Ecology
  • Laboratory Experiments
  • Field Sampling Techniques
  • Species Identification
  • Water Quality Analysis
  • Sample Preservation Methods
  • FDA and EPA Regulations Adherence
  • ddPCR Techniques
  • Gene Expression Analysis
  • Statistical Data Analysis
  • Publishing journal articles
  • cGMP

Internships And Training

  • 2021, Phase I Diversity Training – Online, Racial Equity Institute (REI)
  • 2020, Supervision Essentials: Supervisor Boot Camp – Online, United Employers Association (EAU) Business Direction HR Counsel
  • 2020, Presentation Matters Course, Mississippi State, Ben West
  • 2019, Hands-on Workshop for Pulp and Paper Basics, NC State University, Raleigh, NC
  • 2018, Introduction to Algae Culture and Testing Workshop, University of Waterloo, Waterloo, Ontario, Canada
  • 2016, Bioinformatics Intensive Internship, National Center for Genome Resources, Santa Fe, NM

Presentations

  • Danielle Simning. 2017. Impacts of oil exposure during early life development stages in Sheepshead minnows (Cyprinodon variegatus) under different environmental factors. MS Thesis Defense, The University of Southern Mississippi Gulf Coast Research Laboratory, Ocean Spring, MS
  • Danielle Simning. 2017. Enhanced developmental PAH toxicity under adverse environmental conditions in Sheepshead minnows (Cyprinodonvariegatus). Gulf of Mexico Oil Spill & Ecosystem Science Conference, New Orleans, LA
  • Danielle Simning. 2016. Synergistic suppression of the Aryl Hydrocarbon Receptor and Hypoxia Inducible Factor 1-α signaling pathways after exposure to oil and hypoxia during early life stages of Cyprinodonvariegatus. Mississippi-Alabama Bays and Bayous Symposium, Biloxi, MS
  • Danielle Simning. 2016. Synergist effects of oil exposures and suboptimal environmental conditions during early life stage development in Sheepshead minnows (Cyprinodonvariegatus). Gulf of Mexico Oil Spill & Ecosystem Science Conference, Tampa, FL
  • Danielle Simning. 2016. Evidence for competitive inhibition in Cyprinodonvariegatus in response to hypoxia and oil exposures as a function of age. Society of Environmental Toxicology and Chemistry (SETAC) North American 37th Annual Meeting, Orlando, FL
  • Danielle Simning. 2015. Impacts of oil exposure during early life development stages in Sheepshead minnows (Cyprinodonvariegatus) under different environmental factors. Society of Environmental Toxicology and Chemistry (SETAC) North American 36th Annual Meeting, Salt Lake City, UT

Publications

  • Simning, D. The University of Southern Mississippi. (2017). Impacts of Oil Exposure during Early Life Development Stages in Sheepshead Minnows (Cyprinodon variegatus) under Different Environmental Factors [Master's thesis]. ProQuest Dissertations and Theses Global.
  • Simning, D., Sepulveda, M., De Guise, S., Bosker, T., & Griffitt, R. J. (2019). The combined effects of salinity, hypoxia, and oil exposure on survival and gene expression in developing sheepshead minnows, Cyprinodon variegatus. Aquatic Toxicology, 214, 105234. https://doi.org/10.1016/j.aquatox.2019.105234
  • Jones, E. R., Simning, D., Serafin, J., Sepúlveda, M. S., & Griffitt, R. J. (2020). Acute exposure to oil induces age and species-specific transcriptional responses in embryo-larval estuarine fish. Environmental Pollution, 263, 114325. https://doi.org/10.1016/j.envpol.2020.114325
  • Rodgers, M. L., Simning, D., Sepúlveda, M. S., De Guise, S., Bosker, T., & Griffitt, R. J. (2020). Publisher Correction: Exposure to oil and hypoxia results in alterations of immune transcriptional patterns in developing Sheepshead minnows (Cyprinodon variegatus). Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-61186-w
  • Allmon, E., Serafin, J., Chen, S., Simning, D., Griffitt, R., Bosker, T., De Guise, S., & Sepúlveda, M. S. (2021). The influence of hypoxia on the cardiac transcriptomes of two estuarine species - C. variegatus and F. grandis. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 39, 100837. https://doi.org/10.1016/j.cbd.2021.100837

Timeline

Raw Materials Facility Manager

NextGen Biologics
08.2024 - Current

Quality Control Scientist II

Thermo Fisher Scientific Inc.
01.2022 - 03.2024

Research Scientist and Bioassay Lab Manager

NCASI Inc. Aquatic Biology
09.2017 - 09.2021

Graduate Research Assistant

Marine Toxicology Lab, USM-Gulf Coast Research Lab
01.2015 - 01.2017

Research Technician

Marine Toxicology Lab, USM-Gulf Coast Research Lab
01.2013 - 01.2015

Bachelor of Science - Marine Biology

The University of Southern Mississippi

Master of Science - Ecotoxicology And Coastal Sciences

The University of Southern Mississippi