Summary
Overview
Work History
Education
Careerrelatedexperience
Awards
Publications
Training
Security Clearance
Timeline
Generic

DaRue Prieto

Frederick,USA

Summary

Proactive and goal-oriented professional with excellent time management and problem-solving skills. Known for reliability and adaptability, with swift capacity to learn and apply new skills. Committed to leveraging these qualities to drive team success and contribute to organizational growth. Current DoD TS Security Clearance.

Overview

39
39
years of professional experience

Work History

Blast Injury Research Program Coordinator, Coordinating Contracting

Blast Injury Research Coordinating Office, BIRCO
01.2024 - Current
  • Officer’s Representative (COR), Primary Hand Receipt Holder (PHRH)
  • Coordinator Roles and Responsibilities: Preparation of regular and ad hoc reports for diverse audiences within and external to the Army Medical Research and Development Command (USAMRDC) explaining the objectives and accomplishments of the BIRCO (in support of the Executive Agent for Medical Research for the Prevention, Mitigation, and Treatment of Blast Injuries mission requirements in Public Law 109-163, Sec
  • 256, and Department of Defense (DoD) Directive 6025.21E)
  • Organization, facilitation, and participation in virtual and non-virtual presentations, meetings, conferences, etc
  • Management, preparation, and review of responses to blast injury related taskers in collaboration with the technical contractor staff
  • Participation in the planning, development, and execution of the Military Health System Integrated Blast Injury Prevention Standards Recommendation (MHS BIPSR) process
  • Management and organization of local data drives and the BIRCO SharePoint site
  • COR duties: Management of the BIRCO support services contract with STS Systems Support Services LLC
  • Clarification and guidance of contractor performance with respect to contract specifications and Performance Work Statement (PWS), to include overseeing contractor performance and completing monthly COR reports
  • Adherence to surveillance guidelines for the successful administration of contract services
  • Reviewing and approving invoices
  • Serving as support to the BIRCO Business Integration Officer
  • Primary Hand Receipt Holder duties: Responsible for non-expendable equipment, durable equipment upon issue, and expendables in accordance with Department of Defense Instruction 5000.64, Accountability and Management of DoD Equipment and Other Accountable Property and Army Regulation 710-4: Inventory Management Property Accountability
  • Performance of semi-annual inventories, end-of-life cycle equipment turn-in, purchasing and maintaining accountability of new equipment.
  • Coordinated logistics for research events, including conferences and workshops, fostering networking opportunities among researchers.
  • Stayed abreast of current trends in the field by attending professional development courses and joining relevant industry associations.

11.2022 - 01.2024
  • Joint Trauma Analysis and Prevention of Injury in Combat (JTAPIC), Cognosante, LLC, Medical Business Systems Analyst
  • Organization, facilitation, and participation in virtual and non-virtual presentations, meetings, conferences, etc
  • Coordination of Request for Information (RFI) products and deliverables from inception to completion to include collaboration with JTAPIC/BIRCO partners to build specific datasets for analysis, and to develop final products and deliverables for dissemination and storage
  • Medical records coding to include (1) Review, interpretation, and extraction of injury data from medical records and databases in support of all JTAPIC project areas, (2) Abbreviated Injury Scale (AIS) coding of Service Member (SM) injuries, (3) Assisting in the development of processes for integrating medical information with the proper operational/intelligence and materiel context.

Project Lead

National Biosurveillance Integration Center, NBIC
10.2021 - 04.2022
  • Countering Weapons of Mass Destruction (CWMD), (DHS), TechINT Solutions Group, LLC, for the Biospatial Project
  • The Biospatial platform enables access to health-related EMS data that may provide early warning signs of a biological attack, emerging infectious disease outbreak, or illicit drug overdose event
  • As Project Lead, served as project liaison, defining and resolving operational scientific and technical issues
  • Responsibilities included project tracking, facilitation of meetings, and coordination of project activities
  • Collaborated in the preparation of SOPs, technical reports, projects, and other protected documentation necessary for the advancement of biosurveillance activities.

Research Scientist

Battelle National Biodefense Institute, BNBI
05.2017 - 12.2019
  • National Biodefense and Countermeasures Center (NBACC), , Toxinology
  • Provided technical and laboratory expertise using liquid chromatography high-resolution tandem mass spectrometry (LC- HRMS-MS) and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF/TOF) mass spectrometry
  • Supported bioforensic casework for top governmental agencies by identifying and characterizing protein toxins
  • Provided support in developing, evaluating, validating, and executing mass spectrometry methods
  • Analyzed mass spectrometry data
  • Designed and validated alternate mass spectrometry sample preparation techniques and standard operating procedures (SOPs) for the analysis of protein toxins
  • Generated SOPs and validation reports.

Laboratory of Proteomics and Analytical Technologies, Leidos Biomedical Research Inc
01.2002 - 12.2016
  • FNLCR, (formerly SAIC, NCI), Associate Scientist
  • Managed, implemented, and executed large-scale clinical proteomic biomarker discovery projects, i.e., breast cancer, prostate cancer, renal cell carcinoma (RCC), Barrett's esophagus, simian immunodeficiency virus (SIV), traumatic brain injury (TBI), etc
  • Responsible for all aspects of each project, including experimental study design, clinical sample preparation (especially up-stream processing methods), strong cation exchange (SCX) chromatography, micro capillary reversed-phase liquid chromatography tandem mass spectrometry (µRPLC MS-MS), data analysis, and reporting results to collaborators
  • Published study results in peer- reviewed journals
  • First researchers to develop and apply immunoaffinity depletion of highly abundant blood-derived proteins to fresh-frozen tissue homogenates
  • Trained visiting scientists, collaborators, and summer students.

Senior Research Associate

Laboratory of Immunopathogenesis and Bioinformatics, SAIC, National Cancer Institute, NCI
01.2000 - 12.2001
  • Developed and optimized a telomere length assay designed to measure and compare telomere shortening in chronic lymphocytic leukemia (CLL) and HIV/AIDS patients
  • This technique utilized genomic DNA isolation and assessment, restriction digestion, pulsed-field gel electrophoresis, Southern blotting, and telomere restriction fragment analysis
  • Published study results in peer-reviewed journals.

Research Associate

Immunotherapy Laboratory, SAIC, National Cancer Institute, NCI
04.1997 - 12.1999
  • Supervised an anti-tumor poly nucleotide vaccine (PNV) development project
  • As part of the PNV strategy, developed an agarose-based electrophoretic method, i.e
  • Continuous elution electrophoresis, for selectively isolating and purifying covalently closed circular (CCC) plasmid DNA mixtures
  • This technique utilized plasmid DNA isolation, DNA purification, DNA measurement, and continuous elution electrophoresis
  • Applied polynucleotide vaccine to tumor regression studies in Sprague Dawley rats
  • Executed 2-D SDS-PAGE project focused on the regulation of proteins by thymidylate synthase (TS)
  • Generated peripheral blood monocyte-derived dendritic cells following elutriation of NIH Leukopaks
  • Published study results in peer-reviewed journals.

Technical Services Specialist

BioWhittaker Inc
01.1995 - 03.1997
  • Provided technical support and sales staff training for Clonetics, Bender MedSystems, Cell, and Media product groups
  • Position also required trade show participation in order to introduce customers to available products.

Laboratory Technician

Kemp Biotechnologies
08.1994 - 12.1994
  • Provided expertise in cell culture, protein expression, and protein purification
  • Prepared specialized media and cultured insect cells.

Volunteer Science Teacher

Faith Elementary and Middle School
08.1993 - 06.1994
  • Volunteered to support the Faith Elementary and, Science Program by teaching basic biological principles/practices and by conducting simple experiments with third through eighth-grade students.

University of Delaware, M.S
01.1989 - 01.1992
  • Candidate/Office of Naval Research (ONR) Fellowship Recipient
  • Thesis: Recovery, Respiratory Activity, and Protein Synthesis in Sublethally Chlorine-Injured Enterobacter cloacae 107A Populations
  • Sublethal chlorine injury has been the basis of numerous water- borne disease outbreaks throughout history
  • Studied the effects of sublethal chlorine injury on populations of enteric bacteria in order to monitor several aspects of recovery, i.e
  • Recovery of growth capability on agar plates, respiratory activity, and protein synthesis as measured by 2D SDS-PAGE.

Chemistry Technician

I. Dupont de Nemours and Company
01.1988 - 01.1989
  • 40 Hours/Week
  • E, Prepared standardized DNA sequence sample preparations in order to qualify and release Genesis 2000 DNA Sequencing Systems for sale.

Minority Biomedical Research Support (MBRS), Student Researcher

Delaware State University
01.1986 - 01.1988
  • Cancer Research: Studied the effects of cisplatin and platinum thymine on the kidneys of Swiss Albino mice using transmission electron microscopy (TEM)
  • Prepared and analyzed tissue by TEM and maintained the animal facility
  • Graves’ Disease Research: Organized a new Graves’ disease research laboratory and evaluated human sera for auto-antibodies.

Education

M.S. - Biological Sciences

University of Delaware
01.1992

B.S. - Biology

Delaware State University
01.1988

Careerrelatedexperience

  • 01/2024, Present, Blast Injury Research Program Coordinator, Blast Injury Research Coordinating Office (BIRCO), Preparation of regular and ad hoc reports for diverse audiences within and external to the Army Medical Research and Development Command (USAMRDC)., Organization, facilitation, and participation in virtual and non-virtual presentations, meetings, conferences, etc., Management, preparation, and review of responses to blast injury related taskers in collaboration with the technical contractor staff., Participation in the planning, development, and execution of the Military Health System Integrated Blast Injury Prevention Standards Recommendation (MHS BIPSR) process., Management and organization of local data drives and the BIRCO SharePoint site., Management of the BIRCO support services contract with STS Systems Support Services LLC., Clarification and guidance of contractor performance with respect to contract specifications and Performance Work Statement (PWS)., Reviewing and approving invoices.
  • 11/2022, 01/2024, Medical Business Systems Analyst, Joint Trauma Analysis and Prevention of Injury in Combat (JTAPIC), Cognosante, LLC, Coordination of Request for Information (RFI) products and deliverables from inception to completion., Medical records coding including review, interpretation, and extraction of injury data from medical records.
  • 10/2021, 04/2022, Senior Scientist CBRN, National Biosurveillance Integration Center (NBIC), Department of Homeland Security (DHS), TechINT Solutions Group, LLC, Project Lead for the Biospatial Project., Responsibilities included project tracking, facilitation of meetings, and coordination of project activities.
  • 05/2017, 12/2019, Research Scientist, Toxinology, Battelle National Biodefense Institute (BNBI), National Biodefense and Countermeasures Center (NBACC), Provided technical and laboratory expertise using liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS-MS)., Supported bioforensic casework for top governmental agencies.
  • 01/2002, 12/2016, Associate Scientist, Laboratory of Proteomics and Analytical Technologies, Leidos Biomedical Research Inc., Managed, implemented, and executed large-scale clinical proteomic biomarker discovery projects., Published study results in peer-reviewed journals.
  • 01/2000, 12/2001, Senior Research Associate, Laboratory of Immunopathogenesis and Bioinformatics, SAIC, National Cancer Institute (NCI), Developed and optimized a telomere length assay designed to measure and compare telomere shortening.
  • 04/1997, 12/1999, Research Associate, Immunotherapy Laboratory, SAIC, National Cancer Institute (NCI), Supervised an anti-tumor poly nucleotide vaccine (PNV) development project.
  • 01/1995, 03/1997, Technical Services Specialist, BioWhittaker Inc., Provided technical support and sales staff training.
  • 08/1994, 12/1994, Laboratory Technician, Kemp Biotechnologies, Provided expertise in cell culture, protein expression, and protein purification.

Awards

  • 16th Annual Spring Research Festival Poster Presentation Award, FNLCR & Fort Detrick, 2012
  • Outstanding Contributor of the Quarter, Leidos Biomedical Research Inc., 2010
  • Science Achievement Award, SAIC Frederick, Inc, 2002
  • 6th Annual Spring Research Festival Poster Presentation Award, NCI-Frederick, 2002
  • Department of the Army Certificate of Appreciation, 2000
  • Office of Naval Research Graduate Fellowship, 1989
  • Beta, Beta, Beta Biological Honor Society, 1988
  • Alpha Chi National Honor Society, 1988
  • Phi Alpha Theta International Honor Society, 1988
  • Valerie N. Bush Award for Scholarship, Research and Enthusiasm in Biology, 1988
  • Society for Black Americans Scholarship, 1987
  • Dean’s List, Delaware State University, 1986-1988

Publications

  • Prieto DA, Blonder J., Tissue Sample Preparation for Proteomic Analysis., In: Issaq HJ, Veenstra TD, eds. Proteomic and Metabolomic Approaches to Biomarker Discovery., 2nd ed., San Diego, CA, Elsevier Incorporated, 2020, 47-60
  • Tang W, Zhou M, Dorsey TH, Prieto DA, et al., Integrated Proteotranscriptomics of Breast Cancer Reveals Globally Increased Protein-mRNA Concordance Associated with Subtypes and Survival., Genome Med., 2018, 10, 94-107
  • Prieto DA, Whitely G., Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE., Methods Mol Biol., 2018, 1723, 95-110
  • Prieto DA, Chan KC, Johann DJ, et al., Preparation and Immunoaffinity Depletion of Fresh Frozen Tissue Homogenates for Mass Spectrometry-Based Proteomics in the Context of Drug Target/Biomarker Discovery., Methods Mol Biol., 2017, 1647, 71-90
  • Doumatey AP, Zhou J, Zhou M, Prieto DA, et al., Proinflammatory and Lipid Biomarkers Mediate Metabolically Healthy Obesity: A Proteomics Study., Obesity., 2016, 24, 6, 1257-1265, https://doi.org/10.1002/oby.21482

Training

  • Increasing LC-MS Sensitivity in Protein Analysis by Pre-fractionation, TRAC 33: Clinical Proteomics and Biomarker Discovery, NIH/FAES, Bethesda, MD, 08/2015, 08/2014, 06/2013, 06/2012
  • The Utility of Trypsin-mediated 16O/18O Stable Isotope Labeling in Biological MS-based Proteomics, Mid Atlantic Directors and Staff of Scientific Cores (MAD SSC) 2013 Inaugural Meeting, 06/2013
  • Identification of Potential Plasma Biomarkers of Traumatic Brain Injury Using a Subtractive Proteomics Approach, 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, Indiana, 2007
  • The SELDI-TOF MS Approach to Proteomics, RTP Staff Seminar Series, NCI-Frederick, 2004
  • Serum Proteomic Patterns for Ovarian and Prostate Cancer Detection, Ciphergen Symposium, The Sheraton Inner Harbor, Baltimore, MD, 2003
  • The Long and Winding Road to Purification of pITL: Therapeutic ‘Naked DNA’ at Last, Clinical Services Program Staff Seminar, NCI-Frederick, 1999
  • Recovery, Respiratory Activity and Protein Synthesis in Sublethally Chlorine-Injured Enterobacter cloacae 107A Populations, MBRS Program Seminar, Delaware State University, Dover, DE, 1993

Security Clearance

Department of Defense (DoD) Secret Security Clearance, 01/09/18, Present

Timeline

Blast Injury Research Program Coordinator, Coordinating Contracting

Blast Injury Research Coordinating Office, BIRCO
01.2024 - Current

11.2022 - 01.2024

Project Lead

National Biosurveillance Integration Center, NBIC
10.2021 - 04.2022

Research Scientist

Battelle National Biodefense Institute, BNBI
05.2017 - 12.2019

Laboratory of Proteomics and Analytical Technologies, Leidos Biomedical Research Inc
01.2002 - 12.2016

Senior Research Associate

Laboratory of Immunopathogenesis and Bioinformatics, SAIC, National Cancer Institute, NCI
01.2000 - 12.2001

Research Associate

Immunotherapy Laboratory, SAIC, National Cancer Institute, NCI
04.1997 - 12.1999

Technical Services Specialist

BioWhittaker Inc
01.1995 - 03.1997

Laboratory Technician

Kemp Biotechnologies
08.1994 - 12.1994

Volunteer Science Teacher

Faith Elementary and Middle School
08.1993 - 06.1994

University of Delaware, M.S
01.1989 - 01.1992

Chemistry Technician

I. Dupont de Nemours and Company
01.1988 - 01.1989

Minority Biomedical Research Support (MBRS), Student Researcher

Delaware State University
01.1986 - 01.1988

B.S. - Biology

Delaware State University

M.S. - Biological Sciences

University of Delaware
DaRue Prieto