David McCall

Dynamic professional with extensive experience in protein purification and mass spectrometry at the University of Colorado. Proven track record in curriculum development and laboratory management, fostering team collaboration to enhance educational outcomes. Recognized for innovative approaches in scientific instruction and commitment to student success.

• Apprentice Electrician, Assisted Journeyman Electrician with the installation and maintenance of electrical systems including: Lighting systems, power distribution systems, switches, electrical receptacles, conduits, and high-volt cables
• Front of House Engineer, Developed extensive knowledge of audio equipment and processing, primarily focused on the sound mix as well as onstage monitoring mixes, properly enhanced instrumentation and vocalization of live events, Led setup and tear down of stage equipment, Gained excellent interpersonal skills acting as a liaison between management and performers

• Biophysical Chemistry lab, Developed detailed lab reports on a broad spectrum of topics in physical chemistry, Vibrational spectroscopy, Enzyme kinetics, Thermodynamics of DNA duplexes
• Research Methods Lab, Independently performed research on biochemical processes, Performed background research on bioremediation processes, Designed experimental apparatus for the experimental focus, Designed experimental procedure for testing nitrate levels in aqueous solutions, Prepared reagents for analytical calibration and sample analysis, Analyzed data with analytical calibration methods
• Analytical chemistry lab, Developed detailed lab reports on various analytical chemistry topics, Anodic Stripping Voltammetry using methods of standard additions, Solid Phase Extraction and Gas Phase Chromatography and Mass Spectrometry, Flame Atomic Absorption Spectrophotometry
• Organic Chemistry lab, Developed detailed lab reports on various organic chemistry topics, Thin layer chromatography, Gravity column chromatography, Gas chromatography, High-performance liquid chromatography (HPLC), Ion exchange chromatography, Affinity chromatography, Steric exclusion chromatography

• Professional Research Assistant, 01/01/23, Present, University of Colorado, Boulder - Department of Biochemistry - Ahn Lab, Project lead on the study of structure and dynamics of Extracellular Signal-Regulated Kinase 2, Isolation of plasmid DNA using Qiagen mini-prep kits, Performed protein assays to determine concentration of protein for NMR and Mass Spectrometry experiments, Utilization of transformation techniques of bacterial E. coli cells, Laboratory operation and maintenance of dedicated lab FPLC for protein purification applying techniques of Ion Exchange Chromatography and Size Exclusion Chromatography, Protein purification for analysis using Nuclear Magnetic Resonance Spectroscopy (NMR) and Liquid Chromatography-Mass Spectrometry (LC-MS) to analyze peptides containing phosphorylation sites, Utilization of CCPNMR analysis software to analyze protein samples, Incorporation of highly specialized NOE experiments to study dynamics of ERK2 protein, Search published literature to identify current techniques in protein purification using M9 Minimal Media and deuterium oxide (D20) for the study of isotopically labeled proteins, Operation of Agilent 6530 TOF LC/MS system, Developed protocols for the utilization of UniDec Software to deconvolute mass spectrometry data to determine accurate mass of known and unknown compounds
• Laboratory Leader / Laboratory Scientist, 02/01/22, 01/01/23, Stranaska Scientific, LLC, Laboratory operation and maintenance of higher-order, research-grade computerized analytical UV/VIS/NIR and FTIR spectrophotometers, Execution of science-based UV/VIS spectrophotometric measurement methodologies for the laboratory calibration and recertification of delicate optical filters, Handling and cleaning of delicate cuvette-style filter optics, Review, data entry, spreadsheet analysis, and archival of captured laboratory absorbance data, Inspection of incoming customer standards and completion of inspection forms, Review and proofing of detailed Reports of Calibration, Packaging of standards for return to customer, Creation of measurement service forms for each customer standard received to ensure the appropriate scope of calibration for the recertification request, Search published literature to identify promising technologies and solve problems

Protocol for converting assigned nuclear Overhauser enhancement spectroscopy series peaks into exact distance restraints using MATLAB- or CYANA-eNORA

Morkos A Henen 1, David McCall 2, David Jones 1, Natalie G Ahn 2, Beat Vögeli 3

10.1016/j.xpro.2025.103653

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