Deepshe Dewett-Tuli
Boston,MA
Overview
7
7
years of professional experience
Work History
Graduate Research Assistant
University Of Massachusetts Boston
09.2017 - Current
I investigate the impact of vitamin A deprivation and replacement therapy on the structure and function of the retina at Dr. Jens Rister's lab. I explore the effects on the retina's transcriptome, lipidome, and proteome in collaboration with the Shevchenko lab at MPI Dresden.
Research Intern
Lupin Pharmaceuticals Inc.
01.2017 - 06.2017
I worked in the clone development team. My internship focused on the development of bio-similar drugs through the over-expression of a recombinant protein. I achieved the project's broad objectives of cloning genes into mammalian expression vectors, transfecting cells with recombinant constructs, and generating stable mini-pools, resulting in measurable and detectable expression levels.
Education
Ph.D. - Molecular Cellular And Organismal Biology
University of Massachusetts - Boston
Boston, MA08.2024
Bachelor of Technology - Biotechnology
Dr. D.Y.Patil Biotechnology And Bioinformatics Ins
Pune, India06.2017
Skills
- Academic Research
- Genomics- Cloning
- Transcriptomics- mRNA, circRNA seq, RT-qPCR
- Proteomics- Mass spectrometry
- Protein purification- Co-IP, Affinity chromatography
- Cell culture- Mammalian (CHO, 661W cell lines), Inverterbrate (S2 cell line)
- Immunohistochemistry
- Scientific Communication
- Research design
- Academic Presentations
- Teamwork and Collaboration
- Scientific paper experience
Publications
- Dewett, D., Labaf, M., Lam-Kamath, K., Zarringhalam, K., and Rister, J. (2021). Vitamin A deficiency affects gene expression in the Drosophila melanogaster head. G3: Genes, Genomes, Genetics
- Dewett, D., Lam-Kamath, K., Poupault, C., Khurana, H., and Rister, J. (2021). Mechanisms of vitamin A metabolism and deficiency in the mammalian and fly visual system. Developmental Biology. shared first-author.
- Poupault, C., Choi, D., Lam-Kamath, K., Dewett, D., Razzaq, A., Bunker, J., Perry, A., Cho, I., and Rister, J. (2021). A combinatorial cis-regulatory logic restricts color- sensing Rhodopsins to specific photoreceptor subsets in Drosophila. PLOS Genetics. shared first-author.
- Kumar, M., Has, C., Lam-Kamath, K., Ayciriex, S., Dewett, D., Bashir, M., Poupault, C., Schuhmann, K., Knittelfelder, O., Raghuraman, B.K., Ahrends, R., Rister, J. and Shevchenko, A. (2022). Vitamin A deficiency alters the phototransduction machinery and distinct non-vision-specific pathways in the Drosophila eye proteome. Biomolecules.
- Kumar, M., Has, C., Lam-Kamath, K., Ayciriex, S., Dewett, D., Bashir, M., Poupault, C., Schuhmann, K., Thomas, H., Knittelfelder, O., Raghuraman, B.K., Ahrends, R., Rister, J. and Shevchenko, A. (2023). Eye proteome of Drosophila melanogaster. Proteomics.
- Kumar, M., Has, C., Lam-Kamath, K., Ayciriex, S., Dewett, D., Bashir, M., Poupault, C., Schuhmann, K., Thomas, H., Knittelfelder, O., Raghuraman, B.K., Ahrends, R., Rister, J. and Shevchenko, A. (2023). Lipidome unsaturation affects the morphology and proteome of the Drosophila eye. Journal of Proteome Research.
- Dewett, D., Lam-Kamath, K., Kumar, M., Shevchenko, A., Labaf, M., Zarringhalam, K., Rister, J (2023). Vitamin A deprivation induces a novel transmembrane protein that stabilizes degenerating photoreceptors. Investigative Ophthalmology & Visual Science.
Presentations at conferences
- ‘Mechanisms of photoreceptor stabilization upon chronic vitamin A-deprivation.’ Deepshe Dewett, Khanh-Lam Kamath, Joe Bunker, Fareeha Syeda, Mukesh Kumar, Andrej Shevchenko, Maryam Labaf, Kourosh Zarringhalam, Jens Rister. TAGC 24, March 6-11, 2014.
- ‘Vitamin A deficiency triggers a novel transmembrane protein that stabilizes degenerating photoreceptors.’ Deepshe Dewett, Khanh Lam-Kamath, Ethan Wagner, Mukesh Kumar, Andrej Shevchenko, Maryam Labaf, Kourosh Zarringhalam, Jens Rister. International 64th Annual Drosophila Research Conference, March 1-5, 2023.
- ‘Gene expression changes in Drosophila in response to vitamin A deprivation’. Deepshe Dewett, Maryam Labaf, Khanh Lam-Kamath, Kourosh Zarringhalam, Jens Rister. Northeast Regional Meeting of the Society for Developmental Biology, April 1st, 2022.
- ‘Identifying genes and proteins that respond to Vitamin A deprivation in the eye’, Deepshe Dewett, Khanh Lam-Kamath, Maryam Labaf, Kourosh Zarringhalam, Andrej Shevchenko, and Jens Rister. Midwest Drosophila Conference, Indiana University, October 24, 2020.
- ‘Transcriptional changes in the Drosophila genome during vitamin A deprivation’, Deepshe Dewett and Jens Rister. Boston Area Drosophila Meeting, Boston University, Boston, June 11, 2020.
Affiliations
- Member, Association for Research in Vision and Ophthalmology (ARVO)
- Genetics Society of America (GSA)
- DPU UNESCO Bioethics student unit
Timeline
Graduate Research Assistant
University Of Massachusetts Boston
09.2017 - Current
Research Intern
Lupin Pharmaceuticals Inc.
01.2017 - 06.2017
Ph.D. - Molecular Cellular And Organismal Biology
University of Massachusetts - Boston
Bachelor of Technology - Biotechnology
Dr. D.Y.Patil Biotechnology And Bioinformatics Ins