Delowar Hossain
Fargo,USA
Summary
A sound understanding of molecular biology techniques, mice husbandry, and cell culture. Have four years of experience in method development for chemicals (API) and pharmaceutical products at an R&D laboratory authorized by the US FDA. Meticulous and reliable in analytical method development following compendia (USP, JP, BP) and regulatory guidelines (FDA & ICH), using sophisticated analytical instrumentation such as High-performance liquid chromatography (HPLC), Gas chromatography (GC), UV-Vis spectrophotometer, dissolution apparatus, and so on. Maintained detailed lab records, ensuring compliance with GDP, cGLP and ALCOA. Skilled in technical writing, creating clear and concise documents for industry and academia. Expertise in Empower 3 and chromeleon 7. Demonstrated exceptional interpersonal communication skills, a logical approach, and adept problem-solving abilities to effectively collaborate and resolve complex issues within diverse team environments.
Overview
8
8
years of professional experience
Work History
Graduate Research Assistant
North Dakota State University
2022.01 - Current
- Performing cell lysate preparation, DNA extraction, mRNA isolation, immunoblotting, SDS-PAGE, qRT-PCR, cell culture, subculture, and sterile maintenance of cell lines, isolating epithelial cells and collecting duct cells, tissue fixation, embedding, sectioning, mice care and breeding, genotyping, organ collection, order and purchase laboratory chemicals, materials, and instruments, train interns and new graduate students, effective communication with other labs.
Associate Scientist
Square Pharmaceuticals Limited
2017.09 - 2021.11
- Conducted method development and pre-validation of raw materials and finished products, qualitative and quantitative analysis of raw materials/APIs, in-process and finished pharmaceutical products, analyzed preservative content, assay, dissolution, content uniformity, related substances, elemental impurity, and genotoxic impurity, experienced in data processing of HPLC chromatographs, maintained laboratory record documentation, troubleshooting of analytical methodologies, trained colleagues on analytical equipment and techniques, ensured data integrity and laboratory safety procedures.
Research Associate
Nutrition and Health Research Laboratory
2016.07 - 2017.09
- Preparation of research proposal, research work design and project report preparation, trained new graduate students, laboratory maintenance and management, assisted MS thesis students with research ideas and works.
Education
Master of Science in Pharmaceutical Sciences -
North Dakota State University
03.2024
Master of Science in Biochemistry and Molecular Biology -
University of Dhaka
06.2016
Bachelor of Science in Biochemistry and Molecular Biology -
University of Dhaka
12.2014
Skills
- Analytical method development
- Method pre-validation
- Troubleshooting
- Reverse engineering
- Teamwork and Collaboration
- Cell Culture
- Immunology techniques
- Gene Expression Analysis
- Molecular Biology
- Chromatography Techniques
- HPLC Analysis
- Problem-Solving
Affiliations
- American Association of Pharmaceutical Scientist (AAPS)
- American Society for Biochemistry & Molecular Biology (ASBMB)
- American Society for Nephrology (ASN)
- Graduate Biochemist Association (GBA), Bangladesh
Research
- Currently working on a research project entitled "Sex-specific Expression of Integrin Subunits in the Kidney: Implications for Renal Physiology and Disease." The experimental part includes investigating integrin protein expression in different regions of mice kidneys and analysis by immunoblotting, immunostaining, and qRT-PCR. The primary goal is to investigate the potential role of these Integrin proteins in the progression of kidney disease between sexes., 01/2022, Present, Dr. Sijo Mathew, Department of Pharmaceutical Sciences, North Dakota State University, ND-58102, USA
- Conducted a research work entitled "Association of Glutathione-S-Transferase M1 & T1 gene polymorphisms with Acute Lymphoblastic Leukemia (ALL) in Bangladeshi children.", 01/2015, 06/2016, Dr. Md. Zakir Hossain Howlader, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh.
Presentations
- The higher integrin α5β1 levels in the male kidney cortex indicate a potential reason for the better urine concentration ability in men than women, American Society for Biochemistry and Molecular Biology conference (DiscoverBMB) 2023, Seattle, Washington, 03/2023
- Variable Expression of Integrin Subunits In The Male And Female Kidneys Suggests A Key Factor Responsible For The Higher Prevalence Of Chronic Kidney Diseases In Women, American Heart Association (AHA) Scientific session 2022, Chicago, Illinois, 11/2022
Instrument Skills
- UV-Vis Spectrophotometer
- Confocal Microscopy
- Dissolution Tester
- Atomic Absorption Spectroscopy (AAS)
- Potentiometer
- Gas Chromatography (FID)
- HPLC/UPLC (PDA/DAD, RI, UV)
- FT-IR
- Thin Layer Chromatography (TLC)
- Particle Size Analyzer
- Karl Fischer titration
- PH meter
- Polarimeter
- Viscometer
- Microtome
- Powder X-Ray Diffraction (PXRD)
- Quant studio 3 & 6 Flex (qRT-PCR)
- Azure 600 800 (Western blot developer)
- Cytiva Image quant 800 (Western blot developer)
- NanoDrop (DNA/RNA quantification)
Awards
- ASBMB Travel Award 2023: DiscoverBMB 2023
- Graduate school Travel Award 2023, North Dakota State University
Mailing Address
3315 Broadway N, Apt# 210, Fargo, ND, 58102
Publications
- Integrin β1 is a key determinant of the expression of angiotensin-converting enzyme 2 (ACE2) in the kidney epithelial cells, Eur J Cell Biol, 06/2023, 102, 2, 151316
- The higher integrin α5β1 levels in the male kidney cortex indicate a potential reason for the better urine concentration ability in men than women, Journal of Biological Chemistry, 2023, 299, 3, AP2131, 10.1016/j.jbc.2023.104394
- Variable Expression of Integrin Subunits In The Male And Female Kidneys Suggests A Key Factor Responsible For The Higher Prevalence Of Chronic Kidney Diseases In Women, Hypertension, 2022, AP029
- Association of Glutathione S-Transferase theta 1 and mu 1 Genes Polymorphisms with the Susceptibility of Myocardial Infarction in Bangladesh, Rep Biochem Mol Biol, 2020 Oct, 9, 3, 366-372
- ACE, NOS3 and GST (M1 & T1) genes poly-morphisms and the risk of myocardial infarction in Bangladesh, European Biophysics Journal, 2017, 46, Suppl1, S43-S402
References
Will be furnished on request
Timeline
Graduate Research Assistant
North Dakota State University
2022.01 - Current
Associate Scientist
Square Pharmaceuticals Limited
2017.09 - 2021.11
Research Associate
Nutrition and Health Research Laboratory
2016.07 - 2017.09
Master of Science in Pharmaceutical Sciences -
North Dakota State University
Master of Science in Biochemistry and Molecular Biology -
University of Dhaka
Bachelor of Science in Biochemistry and Molecular Biology -
University of Dhaka
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