DeWayne Threet
Summary
Experienced animal research associate with a proven track record of conducting and analyzing scientific experiments leading to significant research breakthroughs. Proficient in various laboratory techniques and skilled in interpreting complex data sets to drive impactful results. Passionate about contributing to the advancement of scientific knowledge through meticulous research practices.
Overview
32
32
years of professional experience
1
1
Certification
Work History
Animal Research Specialist
Bloodworks Northwest
11.2024 - 04.2025
- Ensured accurate data collection by conducting thorough observations and meticulous recordkeeping.
- Provided training to new associates which included small animal handling, sample collection ( RO Bleeds, Cardiac Puncture, Etc) and dosing( IP, Gavage, Etc).
- Performed general animal care, animal husbandry, facility sanitation and assigned custodial duties.
Research Technician
Seattle Clinical and Biomedical Research Institute (SCBRI)
10.2022 - 10.2024
- Preformed small animal surgery including post-operative monitoring.
- Vascular and ductal cannulation/ Pancreatic Islet isolation.
- Animal perfusion and tissue harvesting.
- Data collection and analyses.
- Actively participated in lab meetings, sharing knowledge and expertise to the group.
- Trained new associates in laboratory procedures and standard.
Research Scientist
UNIVERSITY OF WASHINGTON-DEPT OF ORTHOPAEDICS
08.2004 - 11.2018
- Conducting experiments to identify mechanisms that may control mechanotransduction in bone using a non-invasive loading device.
- Using a number of experimental approaches such as histomorphometry, immunohistochemistry and Micro-CT to identify strategies that lead to potent changes in bone mass.
- Critical in developing a mouse model to test potential osteoporosis treatments.
- Animal colony maintenance/genotyping.
Research Associate II
SNBL USA, LTD
07.1999 - 08.2004
- Performed technical, pre-clinical procedures on research animals (non-human primates and rodents) including, but not limited to, dosing (IV, oral, IM, SC), blood draws using various methods, necropsy or other procedures necessary based on established protocols.
- Accurately recorded test results on standardized forms and wrote test reports describing procedures utilized and data collected.
- Diligently maintained and followed FDA Good Laboratory Practice (GLP) and Standard Operating Procedures (SOP)
Laboratory Technician
Phoenix Central Laboratory (Zoetis Laboratories)
07.1998 - 07.1999
- Performed hematological and chemical analysis using specialized equipment on veterinary samples.
- Issued results to clients in a timely manner.
- Maintained documentation on tests performed in lab.
Laboratory Technician III
Dairy and Food Labs (Silliker Laboratories)
04.1993 - 06.1998
- Performed routine product analysis including bacteriological, chemical, and physical testing of raw and finished goods.
- Peformed pathogenic testing on incoming raw milk or finished products.
- Operated specialized lab equipment to perform microbiological analysis on dairy and food products.
- Maintained a high level of accuracy in test results by following strict quality control procedures.
Trained new associates in laboratory procedures and standard
Education
B.S - Biological Science
California State University
Turlock, CAAssociate's - Nursing
Everett Community College
Everett, WASkills
- Small animal handling and sample collection
- Meticulous experimental conduct (GLP)
- Data collection and analysis
Accomplishments
- Over 25 years of scientific work experience in both industrial and academic settings
- Experienced research scientist proficient with in vivo study design and implementation, data collection and analysis
Certification
Laboratory Animal Technician - LAT
Publications
Worton, L.E., Threet, D., Srinivasan, S., Ausk, B.J., Bain, S.D., Kwon, R.Y.,
Gross, T.S., Gardiner, E.M.,(2022). Beta 2 Adrenergic Receptor Selective Antagonist Enhances Mechanically Simulated Bone Anabolism in Aged Mice. JBMR Plus. 2022 Dec 27;7(2):e10712. doi: 10.1002/jbm4.10712. PMID: 36751418; PMCID: PMC9893264
Srinivasan, S., Threet, D., Worton, L.E., Ausk, B.J., Bain, S.D.,Gardiner, E.M., Kwon, R.Y.,
Gross, T.S. (2014). Distinct cyclosporine A doses are required to enhance bone
formation induced by cyclic and rest-inserted loading in the senescent Skeleton.
PLOS One Jan3;9(1):e84868 PMID: 18280231
Srinivasan, S., Ausk B.J., Prasad J., Threet D., Bain S.D., Richardson T.S., Gross T.S.
(2010). Rescuing Loading Induced Bone Formation at Senescence. PLoS Comput
Biol, 6(9): e1000924. PMID:20838577
Poliachik, S.L., Bain, S.D., Threet, D., Huber, P. Gross, T.S. (2010). Transient muscle
paralysis disrupts bone homeostasis by rapid degradation of Bone. Bone, 46:18-23.
PMID: 19857614
Poliachik, S.L., Threet, D., Srinivasan, S., Gross, T.S. (2008). 32 wk old C3H/H3J mice
actively respond to mechanical Loading. Bone, 42(4):653-659 PMID: 24404194
2014 Orthopaedic Research Society
Ausk, B.J., Worton, L.E., Threet, D., Kwon, R.Y., Bain, S.D., Gardiner, E.M., Gross, T.S.
(2014). Transient muscle paralysis predisposes bone marrow to formation of giant
Osteoclasts.
Stoll, L., Huber, P., Kwon, R.Y., Threet, D., Gross, T.S., Bain, S.D. (2014). Contralateral
bone loss following ipsilateral nerve injury: Evidence for a neuronal Skeletome.
2013 Orthopaedic Research Society
Kwon, R.Y., Threet, D., Gardiner, E.M., Worton, L.E., Gross, T.S., Srinivasan, S (2013).
Timed administration of cyclosporin A supplements reveals multiple routes to
rescuing loading induced bone formation at Senescence.
Biomedical Engineering Society.
Timeline
Animal Research Specialist
Bloodworks Northwest
11.2024 - 04.2025
Research Technician
Seattle Clinical and Biomedical Research Institute (SCBRI)
10.2022 - 10.2024
Research Scientist
UNIVERSITY OF WASHINGTON-DEPT OF ORTHOPAEDICS
08.2004 - 11.2018
Research Associate II
SNBL USA, LTD
07.1999 - 08.2004
Laboratory Technician
Phoenix Central Laboratory (Zoetis Laboratories)
07.1998 - 07.1999
Laboratory Technician III
Dairy and Food Labs (Silliker Laboratories)
04.1993 - 06.1998
Associate's - Nursing
Everett Community College
B.S - Biological Science
California State University
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