Doha Sleem

• Assisted in research uncovering faithful chromosomal segregation in mitosis.
• Achievements:
• · Preparation of plasmid for genomic editing through molecular cloning techniques.
• Responsibilities:
• · Conduct cell culture procedures with strict adherence to aseptic techniques.
• · Systematically observed temperature, pH levels, cell viability, and cell density with precision to uphold quality standards.
• · Perform cell counting by hemocytometer.
• · Store cell lines in liquid nitrogen.
• · Perform Immunostaining procedures and cell imaging by fluorescence microscopy.
• · Participate in journal club article.
• Train undergrads on laboratory safety and settings.
• Executed research focused on the expression and purification of major and minor translocon proteins of Gram-negative bacteria.
• Responsibilities:
• · Perform bacterial transformation using plasmids IpaB TM1 TM2 310-423, IpaC TM 99-120 and SipB TM 320-428
• · Conduct small- and large-scale protein expression
• · Perform Ni Column Chromatography
• Achievements:
• · Effectively communicated research findings through poster and oral presentations at K-INBRE.

Conducted ecotoxicological and biological field investigations on venomous and non-venomous snakes in El-Faiyum desert, Egypt.

Responsibilities

· Digest heavy metals in tissues and soil by microwave and measure them by ICAP.

· Perform histological techniques and cell staining by hematoxylin and eosin.

· Perform SDS-PAGE.

· Conduct hematological assays.

· Performed experiments on immunoserums and antibodies sourced from rabbits, employing a range of assays such as immunoassays, Western Blot, and ELISA to analyze antigens and antibodies.

· Executed protein purification via HPLC under senior researcher supervision, while meticulously preparing chromatography columns, buffers, and samples in accordance with established protocols.

· Clean and sterilize glassware through washing and autoclaving processes.

· Prepare reagents and solutions essential for experiments.

· Manage inventory by ordering and restocking supplies as needed.

· Ensure cleanliness and organization within the laboratory environment.

· Execute bacterial sterile techniques to maintain aseptic conditions.

· Maintain meticulously organized and up-to-date laboratory notebooks.

· Keep comprehensive records of experiments and collected data.