Drishti Rohatgi

· Experience in analytical experiments to characterize purified proteins (aSEC, aCEX, aHIC, LabChip)

· Developed and validated protein stabilization methods through independent research and small-scale experimentation; methods were implemented across multiple teams

· Performed ligand binding assays (e.g., SPR, ELISA-based, fluorescence-based) to characterize protein–ligand interactions.

· Troubleshooted and resolved bioanalytical method issues

· Established and optimized high-throughput and automated methodologies for protein purification as needed (Andew+ pipetting robot, KingFisher & HisMultiTrap)

· Performed next generation sequencing methods i.e., PCR, library preparation, sample purification, and operation and maintenance of sequencing platforms (BioEdit and Geneious)

· Maintained mammalian cell cultures, perform cell transfections for recombinant protein expression on large and small scale

· Conducted bench or pilot-scale laboratory experiments to develop, characterize, and/or validate cell culture processes

· Mentored and trained new employees

· Optimized current immunoassay to improve results while reducing cost by nearly 70%.

· Owned, juggled and advanced multiple projects and tasks to ensure high quality and timely delivery (project management).

· Written multiple SOPs that have been introduced into manufacturing and production scale.

· Outlined, revised and executed experiments in laboratory environment for product development of a rapid COVID-19 Assay.

· Prepared cell culture media to grow cells using GMP to sustain a sterile and healthy environment.

· Worked closely with RNA viruses and virus titration

· Directed bioinformatics processes to compare different variants of viruses and bacteria for diverse internal and external research.

· Aware of and compliant with clean room practices, oligo preparation and basic liquid handling