Dulce Suarez

Student graduate with 3 years of lab experience. Seeking to implement research, data collection, collaboration skills, and extensive knowledge of testing and analysis in a lab setting. Eager to pursue a career in research in hopes of obtaining a doctoral degree in Pharmacy and entering a pharmaceutical company.

CRISPR

DNA Extraction

PCR

Gel Electrophoresis

Biochemical Testing

Aseptic Techniques

Culturing Technique

Culturing Techniques

Site-directed Mutagenesis

Protein Assays

Plasmid extraction and purification

Microscopy

April 2023 Adult and Pediatric First Aid/CPR/AED Course Completion

February 2022 CITI Program Course Completion

December 2023 BLS Course Completion

Senior Research | Title: Effects of a Point Mutation in FUS1 on Cell Fusion in Saccharomyces cerevisiae

March 2022- April 2023

• Designed lab experiment and perform research into topic to increase knowledge
• Follow lab protocols to complete designated experiments
• Observe and collect data using Nikon Clarify software on yeast cell fusion with efficacy to support research objective
• Organized data in Excel and interpret results with chi-square test
• Created powerpoint to present research at Showcase to professors and new student researchers

Cell Biology Research | Title: Effects of Quercetin on MRC-5 Lung Myofibroblasts involving  TGF-β

August 2022-December 2022

• Identify potential drug targets in diseases (lung fibrosis)
• Research natural remedy (quercetin) effects on the body
• Create hypothesis and devise lab experiments (cell death assay, protein assay, and western blot)
• Used powerpoint and excel to create graphs and visuals of lab to demonstrate our research

Molecular Biology and Biotechnology | CRISPR of Optix in Vanessa Cardui and WntA CRISPR Phenotype Analysis

January 2022-April 2022

• Analyze the use of CRISPR-cas9 on diseases and organ transplants
• Extend knowledge of CRISPR to gene editing of butterfly wing patterns
• Microinjected CRISPR into butterfly eggs
• Use PCR to amplify optix gene of wild type and mutant and ran DNA agarose gel
• Sequence analysis of PCR product from CRISPR mutants with wild type and reference gene from database using Snapgene software
• Provided care to specimens from egg to fully grown butterfly to observe difference between wild type and mutant CRISPR butterfly wing patterns