Ebru Robinson
San Diego,CA
Summary
A Molecular Biologist with a background as a Licensed Physiotherapist, specializing in gene therapy, bioinformatics data analysis, muscle and nervous system regulation, and their associated diseases.
Overview
7
7
years of professional experience
Work History
Teaching Assistant
San Diego State University
San Diego, CA
01.2023 - Current
- 366L Biochemistry, Cell, and Molecular Biology Laboratory Course
- I teach mid-level molecular biology experiments as well as Bioinformatics research including Blast search, primer design, and cloning.
- I hold office hours, attend preparatory meetings, and collaborate with the lead professor in developing the course.
Intern Research Associate III
Insmed
San Diego
05.2023 - 08.2023
- I performed AAV9 transduction and tissue culture experiments to administer gene therapy to astrocyte cells derived from ALS patients and skeletal muscle cells from individuals with muscular dystrophy. I reprogrammed skin fibroblast cells into neuro progenitor cells (iNPC) and then differentiated them into astrocyte cells. To confirm the reprogrammed cells' phenotype, I conducted immunostaining using pathological markers.
- For an in-depth analysis of lysosomal and mitochondrial changes, along with protein aggregations in treated and untreated astrocyte and skeletal muscle cells, I utilized live cell staining (LysoTracker, MitoTracker), coculture staining, immunostaining, and optimized antibody techniques.
- To investigate motor neuron survival and neurotoxicity, I employed coculture with astrocyte cells. Biodistribution studies were conducted through spinal cord cryosectioning with DAB, H&E staining, ELISA, and Laminin/Dystrophin staining.
- In the quantification of vector genomes in muscle tissues from muscular dystrophy patients, I utilized ddPCR. Additionally, to measure micro-dystrophin protein expression in treated muscle cells, I followed a protocol involving BSA and Jess. Proteins were extracted from both heart and skeletal muscle for the Pierce BSA protein assay.
Research Assistant III
Department of Biology, San Diego State University
San Diego
01.2022 - 01.2023
- Generated a multi-tag positive control protein for expression, purification, mammalian and drosophila cell transfection, Western Blotting. This involved conducting protein purification, western blotting, glycoprotein staining, immuno-precipitation, bacterial transformation, Gel electrophoresis, Microscopy, PCR, and Sanger Sequencing.
- Conducted CRISPR gene editing technology to create mutant alleles in Drosophila flight muscles. This research included a reverse genetic screening process, cryosectioning drosophila flight muscles, immunostaining, protein expression and purification for antibody production, fly tests, and pull-down assay to look at the physical interaction of knocked-down proteins with other Z-disc proteins. For the same project, I conducted a CRISPR gene tagging experiment to find the location of the protein of interest.
- Operated Transmission Electron Microscopy and Confocal Microscopy for the analysis of Drosophila flight muscle structure for mutation analysis purposes.
- Analyzed ESTs, proteins, genomic DNA, and cDNA databases using Blast and ClustalW to identify related proteins and nucleic acids.
- Managed hiring and laboratory work orders efficiently.
Staff Volunteer
Department of Biology, UC Berkeley
Berkeley, CA
02.2020 - 05.2020
- CRISPR Genome Editing: From Biology to Technology Data Analysis for Genomics Characterization of Secondary Metabolites Produced by Newly Isolated Flavobacterium sp.
Research Assistant I
PROMER
Istanbul, Turkiye
01.2017 - 05.2019
- Analyzed clinical samples on LC/MS-MS, resolving unusual data trends and handling chromatography and instrument alarms as appropriate
- Isolation of mitochondria, UV and Vis spectrometry, microarray analysis, optical, and fluorescence microscopy: phase contrast imaging, time-lapse video microscopy, Live/Dead assay, DAPI staining.
Staff Volunteer
Private Doga College
Istanbul, Turkiye
06.2018 - 08.2018
- Assisted the lab operations by performing molecular biology/microbiology experiments.
Intern Molecular Biologist
Medical Park Hospitals Group
Istanbul, Turkiye
01.2017 - 08.2017
- Assisted with In Vitro Fertilization (IVF) Procedures such as human embryo transfers, egg fertilization, egg retrieval, sperm injection (ICSI), assisting hatching (AH), and sperm preparation
- Daily assessment and grading of the health and development of embryos
- Studied sperm count, and sperm mobility, and performed anti-sperm antibody testing.
Education
M.Sc. - Cell and Molecular Biology
San Diego State University
B.Sc. - Molecular Biology and Genetics (English)
Uskudar University
06.2019
B.Sc. - Physical Therapy and Rehabilitation
Uskudar University
06.2019
Skills
- Transmission Electron Microscopy (TEM)
- Image J
- AAV9 Transduction
- Mammalian, Bacterial and Drosophila Cell Culture
- CRISPR
- Protein Purification and Analysis
- Cryosectioning and Immunostaining
- Jess Western Blot, ELISA, ddPCR
- Muscle Anatomy and Neurodegenerative Diseases
Publications
Robinson E, Alonso E, Waters JA, Bileckyj C, House CD, Johnston CA, and Cripps RM. pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots. Biotechniques. In revision.
Awards
- Certificate of High Achievement (6 terms)
- Certificate of Honor Student
- Received a full scholarship in Molecular Biology and Genetics, Physical Therapy and Rehabilitation at Uskudar University
Volunteer Positions
- Muscular Dystrophy Association (Muscle Walks, Virtual Volunteer and Fundraising event/campaign)
- Volunteer Coach, Foundation for Children with Leukemia
- Project Leader, Uskudar University Bio-Genius Club
- Project Leader, Uskudar University Informatics Club
Credentials
- Fundamentals of Neuroscience- Harvard University
- GBA (Young Brain Academy Application and Research Center), Project writing and management training, Uskudar University
- Training of Small and Medium Enterprises Development Organization
Teaching and Mentoring, San Diego State University
· Sarah Mason (B.S. Student 2022- Present)
· Qusay Lateef (B.S. Student 2022- 2023)
Timeline
Intern Research Associate III
Insmed
05.2023 - 08.2023
Teaching Assistant
San Diego State University
01.2023 - Current
Research Assistant III
Department of Biology, San Diego State University
01.2022 - 01.2023
Staff Volunteer
Department of Biology, UC Berkeley
02.2020 - 05.2020
Staff Volunteer
Private Doga College
06.2018 - 08.2018
Research Assistant I
PROMER
01.2017 - 05.2019
Intern Molecular Biologist
Medical Park Hospitals Group
01.2017 - 08.2017
M.Sc. - Cell and Molecular Biology
San Diego State University
B.Sc. - Molecular Biology and Genetics (English)
Uskudar University
B.Sc. - Physical Therapy and Rehabilitation
Uskudar University
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