Summary
Overview
Work History
Education
Skills
Websites
Awards
Timeline
Generic

Eli Scharlat

Elizabeth,,NJ

Summary

Detail-oriented scientist with 3+ years of hands-on molecular biology and research experience. Passionate about scientific literature and communication. Interested in utilizing my skills and experience towards a career in science and research.

Overview

5
5
years of professional experience

Work History

Lab Technician

Genscript
01.2024 - Current
  • Utilizing molecular biology techniques, bioinformatics software and automation equipment to screen plasmid constructs as part of the Gene Assembly department of GenScript’s production lab
  • Processes and screens 300+ plasmid orders daily using colony PCR, gel electrophoresis and SnapGene software, as well as laboratory automation systems such as Tecan liquid handlers and QPIX colony picking machines
  • Performs plasmid ligation reactions such as Golden Gate and Gibson assemblies including bacterial transformation and plating
  • Provides feedback to and collaborates with other teams in the Gene Assembly department to troubleshoot DNA constructs and optimize production
  • Documents screening results and data using Excel and Manufacturing Execution System (MES) for use by the Plasmid Ligation and Vector teams in the Gene Assembly department

Researcher

Papathanos Lab, Hebrew University of Jerusalem
01.2022 - 01.2023
  • Completed an independent research project and authored a developing a sex-specific splicing genetic tool in A. gambiae mosquitos utilizing bioinformatics and molecular biology techniques in a molecular insect genetics research lab
  • Authored a research proposal and experimental plan which was presented to and approved by the Department Chair and PI of the lab
  • Analyzed scientific literature which informed the development of innovative sex-specific splicing genetic tools in A. gambiae
  • Designed DNA constructs utilizing Geneious bioinformatics software and online databases such as NCBI and Vectorbase, and used molecular cloning, including Golden Gate and Gibson assembly reactions, to synthesize genetic constructs
  • Lead a microinjection team to inject the genetic constructs in A. gambiae embryos to produce transgenic strains
  • Composed a final report documenting the experimental process and interpreting the results, contributing to the further development of insect genetic control tools

Research Assistant

Papathanos Lab, Hebrew University of Jerusalem
01.2020 - 01.2023
  • Maintained and screened wild type and transgenic mosquito colonies and utilized molecular biology workflows to contribute to research in a molecular genetics research lab
  • Assisted researchers in managing and carrying out experiments across several research teams using molecular techniques such as DNA isolation/purification, gel electrophoresis, PCR and restriction digests as well as standard/fluorescent microscopes to produce data towards developing genetic control tools in mosquitos
  • Prepared lab reagents/materials, including bacterial media plates, glycerol stocks, and ATP aliquots utilizing biological fume hoods, autoclaves and analytic scales and maintained and logged lab inventory
  • Mentored and trained 10+ new hires in molecular biology techniques, reagent preparation and management, and microscopy

Education

B.SC - Agroecology & Plant Health, Nature Conservation & Landscape Management

Hebrew University of Jerusalem
01.2023

Skills

  • PCR & Gel Electrophoresis
  • Geneious & SnapGene Software
  • Molecular Cloning
  • Golden Gate Assembly
  • Gibson Assembly
  • DNA Extraction & Purification
  • Pipetting/Micro-pipetting
  • Microsoft Office
  • Molecular Biology Workflows
  • Gene Design & Assembly
  • Laboratory Maintenance
  • Reagent Preparation
  • Scientific Literature Review
  • Experimental documentation
  • Team collaboration
  • DNA sequencing
  • Microscopy

Awards

MAgna Cum Laude Honors, Hebrew University of Jerusalem, 2023

Timeline

Lab Technician

Genscript
01.2024 - Current

Researcher

Papathanos Lab, Hebrew University of Jerusalem
01.2022 - 01.2023

Research Assistant

Papathanos Lab, Hebrew University of Jerusalem
01.2020 - 01.2023

B.SC - Agroecology & Plant Health, Nature Conservation & Landscape Management

Hebrew University of Jerusalem
Eli Scharlat