Detail-oriented scientist with 3+ years of hands-on molecular biology and research experience. Passionate about scientific literature and communication. Interested in utilizing my skills and experience towards a career in science and research.
Overview
5
5
years of professional experience
Work History
Lab Technician
Genscript
01.2024 - Current
Utilizing molecular biology techniques, bioinformatics software and automation equipment to screen plasmid constructs as part of the Gene Assembly department of GenScript’s production lab
Processes and screens 300+ plasmid orders daily using colony PCR, gel electrophoresis and SnapGene software, as well as laboratory automation systems such as Tecan liquid handlers and QPIX colony picking machines
Performs plasmid ligation reactions such as Golden Gate and Gibson assemblies including bacterial transformation and plating
Provides feedback to and collaborates with other teams in the Gene Assembly department to troubleshoot DNA constructs and optimize production
Documents screening results and data using Excel and Manufacturing Execution System (MES) for use by the Plasmid Ligation and Vector teams in the Gene Assembly department
Researcher
Papathanos Lab, Hebrew University of Jerusalem
01.2022 - 01.2023
Completed an independent research project and authored a developing a sex-specific splicing genetic tool in A. gambiae mosquitos utilizing bioinformatics and molecular biology techniques in a molecular insect genetics research lab
Authored a research proposal and experimental plan which was presented to and approved by the Department Chair and PI of the lab
Analyzed scientific literature which informed the development of innovative sex-specific splicing genetic tools in A. gambiae
Designed DNA constructs utilizing Geneious bioinformatics software and online databases such as NCBI and Vectorbase, and used molecular cloning, including Golden Gate and Gibson assembly reactions, to synthesize genetic constructs
Lead a microinjection team to inject the genetic constructs in A. gambiae embryos to produce transgenic strains
Composed a final report documenting the experimental process and interpreting the results, contributing to the further development of insect genetic control tools
Research Assistant
Papathanos Lab, Hebrew University of Jerusalem
01.2020 - 01.2023
Maintained and screened wild type and transgenic mosquito colonies and utilized molecular biology workflows to contribute to research in a molecular genetics research lab
Assisted researchers in managing and carrying out experiments across several research teams using molecular techniques such as DNA isolation/purification, gel electrophoresis, PCR and restriction digests as well as standard/fluorescent microscopes to produce data towards developing genetic control tools in mosquitos
Prepared lab reagents/materials, including bacterial media plates, glycerol stocks, and ATP aliquots utilizing biological fume hoods, autoclaves and analytic scales and maintained and logged lab inventory
Mentored and trained 10+ new hires in molecular biology techniques, reagent preparation and management, and microscopy