Eliana Estrada

Engaged in vivo activities directed toward the discovery and characterization of novel nanoparticles and other proprietary systems for delivering nucleic acids and proteins for in vivo gene therapy

• Conduct mouse studies to support in vivo screening and pharmacology projects
• Hands-on expertise in routine small animal techniques, particularly tail vein injection, retro-orbital bleeding, submandibular venipuncture, induction/maintenance of anesthesia, whole body perfusion, and necropsy/tissue collection
• Execute routine pharmacological endpoint assays, in vivo bioluminescence imaging (BLI), luciferase activity, flow cytometry, and high-content imaging
• Assist with the management of day-to-day vivarium operations, including interfacing with vivarium/husbandry staff, colony management, and recordkeeping following USDA, PHS, and AAALAC guidelines.
• Directed all vivarium studies, reporting to senior management to provide insights and recommendations for enhancing throughput, optimizing space planning, improving animal care, and refining in vivo optimization
• Skilled at managing multiple projects simultaneously

Execute basic and intermediate surgical procedures, provide related animal care, and manage administrative tasks to meet client requirements.

• Assist in training and developing junior surgical team members and less experienced surgeons, providing guidance and oversight as needed.
• Perform basic and intermediate surgical procedures, as assigned
• Deliver comprehensive pre- and post-operative care for animals.
• Perform blo-specimen collection associated with surgical procedures
• Perform all duties following the prescribed safety environment
• Adhere to all safety protocols and maintain a clean, healthful, and safe working environment
• Ensure accurate record-keeping through documentation and establishing a reliable paper trail.
• Oversee work assignments and provide support to junior team members.

Conduct a range of laboratory procedures in accordance with Good Manufacturing Practice (GMP) regulations, ensuring compliance in all aspects of nonclinical research activities.

• Administer daily test articles/substances and record study parameters as outlined in the protocol
• Document study parameters accurately in compliance with protocol requirements.
• Assist in preparing data forms for specialized study procedures.
• Prepare and formulate test agents to maintain an adequate supply of dosages throughout the study.
• Mentor and train department technicians and technologists, and assist in coordinating departmental activities and planning

• Conduct daily animal health observations and perform animal husbandry duties to monitor and support animal health and welfare.
• Assist in the safe restraint, handling, and manipulation of various animal species as needed.
• Ensure proper disposal of animal carcasses and waste in compliance with health and safety protocols.
• Maintain animal housing areas according to established procedures to ensure a clean and suitable environment.
• Receive, inspect, and house incoming animals appropriately, following all guidelines for animal care.
• Sanitize, maintain, and repair caging materials as directed, and keep cage wash facilities clean and well-organized.
• Coordinate with client representatives and management to uphold quality compliance practices in study conduct, data collection, and reporting.
• Address customer requests, inquiries, and complaints, and provide technical assistance as directed.
• Perform technical procedures including blood and tissue collection, injections, surgical prep, anesthesia administration, and surgical assistance.
• Conduct breeding and weaning procedures to maintain adequate production levels.

• Perform animal husbandry and support services following established procedures.
• Review animal health reports for abnormal conditions and provide basic clinical treatments as directed by a veterinarian, Laboratory Animal Program Manager (LAPM), or designated personnel.
• Document, organize, and maintain accurate, timely records of all activities.
• Oversee autoclave and sanitation operations, including quality assurance and surveillance of facility hygiene.
• Ensure compliance with all relevant regulations and standards.
• Complete and submit monthly census reports.
• Perform toe and nail clips for genetic analysis and ear tagging mice for identification purposes
• Technical aspects of breeding including, weaning, separation, mating, and pup checks
• Administered oral medication to animals on treatment
• Perform oral exam and do teeth trimming as needed
• Perform necropsies as requested
• Framingham, MA

• Support experimental surgical or technical procedures on multiple species, including dose administration, sample collection, euthanasia, necropsy, anesthesia, surgical preparation and recovery
• Perform health assessments, analgesic management, and medical care under veterinary supervision
• Maintain procedure rooms with appropriate supplies and sanitize as necessary
• Ensure compliance with internal and external regulations and guidelines following facility standards
• Maintain accurate documentation and research confidentiality

• Complete animal husbandry and support services according to standard procedures
• Evaluate animal health report of abnormal conditions and monitor and provide simple clinical treatments as directed by a veterinarian, LAPM, or designer
• Document, organize, and maintain timely records for activities
• Oversee autoclave and sanitation operations including facility quality assurance or surveillance
• Ensure compliance with required regulations
• Complete census reports monthly
• Perform toe and nail clips for genetic analysis and ear tagging mice for identification purposes
• Technical aspects of breeding including, weaning, separation, mating, and pup checks
• Administered oral medication to animals on treatment
• Perform oral exam and do teeth trimming as needed
• Perform necropsies as requested
• Framingham, MA

• Complete animal care, husbandry, and support services
• Evaluate and monitor animal health
• Handle, restrain, provide care, and observe the general health of all species of laboratory animals
• Ensure compliance with required regulations
• Experience with large animals and rodents

• Proficient in handling, restraining, material administration (IP), tapping and blood collection for rodents
• Knowledgeable in husbandry and inventory
• Administered various therapeutic measures by IV, SQ
• Proficient in aliquot
• Reviewed data for quality control purposes
• Handled, restrained, provided care, and observed the general health of all species of laboratory animals at the site

• Chosen to participate in planning/coordination of customer projects for the department
• Served as a lead in specific departmental projects
• Completed animal husbandry and inventory according to standard procedures
• Served as a mentor to less experienced technicians and junior staff
• Proficient in highly specialized techniques such as tattooing, surgery, packing and shipping animals, ovariectomy, castration, and vasectomy procedures
• Strictly followed procedures necessary to maintain isolators